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Q4WS33

- BGALA_ASPFU

UniProt

Q4WS33 - BGALA_ASPFU

Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (13 Jul 2010)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
    Binding sitei142 – 1421SubstrateBy similarity
    Binding sitei199 – 1991SubstrateBy similarity
    Active sitei200 – 2001Proton donorSequence Analysis
    Binding sitei260 – 2601SubstrateBy similarity
    Active sitei298 – 2981NucleophileSequence Analysis
    Binding sitei364 – 3641SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase A (EC:3.2.1.23)
    Alternative name(s):
    Lactase A
    Gene namesi
    Name:lacA
    ORF Names:AFUA_1G14170
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000002530: Chromosome 1

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 1006988Probable beta-galactosidase APRO_0000395217Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi205 ↔ 206By similarity
    Disulfide bondi266 ↔ 315By similarity
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi760 – 7601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi777 – 7771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5085.CADAFUAP00007691.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4WS33.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    KOiK01190.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4WS33-1 [UniParc]FASTAAdd to Basket

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    MKLLSVCAIA LLAAQAAGAS IKHMLNGFTL MEHSDPAKRE LLQKYVTWDE     50
    KSLFVNGERI MIFSGEVHPF RLPVPSLWLD VFQKIKALGF NCVSFYVDWA 100
    LLEGKPGEYR AEGNFALEPF FDVAKQAGIY LLARPGPYIN AEASGGGFPG 150
    WLQRVNGTLR TSDPAYLKAT DNYIAHVAAT IAKGQITNGG PVILYQPENE 200
    YSGACCDATF PDGDYMQYVI DQARNAGIVV PLINNDAWTG GHNAPGTGKG 250
    EVDIYGHDSY PLGFDCGHPS VWPKGNLPTT FRTDHLKQSP TTPYSLIEFQ 300
    AGSFDPWGGP GFAACAALVN HEFERVFYKN DLSFGAAILN LYMTFGGTNW 350
    GNLGHPGGYT SYDYGSPLTE SRNVTREKYS ELKLIGNFVK ASPSYLLATP 400
    GNLTTSGYAD TADLTVTPLL GNGTGSYFVV RHTDYTSQAS TPYKLSLPTS 450
    AGRLTVPQLG GTLTLNGRDS KIHVVDYNVA GTNIIYSTAE VFTWKNFGDS 500
    KVLILYGGPG EHHELAVSLK SDVQVVEGSN SEFKSKKVGD VVVVAWDVSP 550
    SRRIVQIGDL KIFLLDRNSV YNYWVPQLDK DDSSTGYSSE KTTASSIIVK 600
    AGYLVRTAYT KGSGLYLTAD FNATTPVEVI GAPSNVRNLY INGEKTQFKT 650
    DKNGIWSTEV KYSAPKIKLP SMKDLDWKYL DTLQEVQSTY DDSAWPAADL 700
    DTTPNTLRPL TTPKSLYSSD YGFHTGYLIY RGHFVADGSE TTFDVRTQGG 750
    SAFGSSVWLN ESFLGSWTGL NANADYNSTY KLPQVEQGKN YVLTILIDTM 800
    GLNENWVVGT DEMKNPRGIL SYKLSGRDAS AITWKLTGNL GGEDYQDKIR 850
    GPLNEGGLYA ERQGFHQPQP PSQKWKSASP LDGLSKPGIG FYTAQFDLDI 900
    PSGWDVPLYF NFGNSTKSAY RVQLYVNGYQ YGKFVSNIGP QTSFPVPQGI 950
    LNYQGTNWVA LTLWALESDG AKLDDFELVN TTPVMTALSK IRPSKQPNYR 1000
    QRKGAY 1006
    Length:1,006
    Mass (Da):110,399
    Last modified:July 13, 2010 - v2
    Checksum:i9ECBFA8B0F102FBB
    GO

    Sequence cautioni

    The sequence EAL90749.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000004 Genomic DNA. Translation: EAL90749.1. Sequence problems.
    RefSeqiXP_752787.1. XM_747694.1.

    Genome annotation databases

    EnsemblFungiiCADAFUAT00007691; CADAFUAP00007691; CADAFUAG00007691.
    GeneIDi3509797.
    KEGGiafm:AFUA_1G14170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000004 Genomic DNA. Translation: EAL90749.1 . Sequence problems.
    RefSeqi XP_752787.1. XM_747694.1.

    3D structure databases

    ProteinModelPortali Q4WS33.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5085.CADAFUAP00007691.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFUAT00007691 ; CADAFUAP00007691 ; CADAFUAG00007691 .
    GeneIDi 3509797.
    KEGGi afm:AFUA_1G14170.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    KOi K01190.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
      Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
      , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
      Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

    Entry informationi

    Entry nameiBGALA_ASPFU
    AccessioniPrimary (citable) accession number: Q4WS33
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3