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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei141Substrate; via amide nitrogenBy similarity1
Binding sitei142SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorSequence analysis1
Binding sitei260SubstrateBy similarity1
Active sitei298NucleophileSequence analysis1
Binding sitei364SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:AFUA_1G14170
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu1g14170

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000039521719 – 1006Probable beta-galactosidase AAdd BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi622N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi760N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi777N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi914N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ4WS33

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00001344

Structurei

3D structure databases

ProteinModelPortaliQ4WS33
SMRiQ4WS33
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922
InParanoidiQ4WS33
KOiK01190
OrthoDBiEOG092C0SLI

Family and domain databases

Gene3Di2.102.20.10, 1 hit
2.60.120.260, 3 hits
2.60.390.10, 1 hit
InterProiView protein in InterPro
IPR018954 Betagal_dom2
IPR037110 Betagal_dom2_sf
IPR025972 BetaGal_dom3
IPR036833 BetaGal_dom3_sf
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF10435 BetaGal_dom2, 1 hit
PF13363 BetaGal_dom3, 1 hit
PF13364 BetaGal_dom4_5, 2 hits
PF01301 Glyco_hydro_35, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SMARTiView protein in SMART
SM01029 BetaGal_dom2, 1 hit
SUPFAMiSSF117100 SSF117100, 1 hit
SSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WS33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSVCAIA LLAAQAAGAS IKHMLNGFTL MEHSDPAKRE LLQKYVTWDE
60 70 80 90 100
KSLFVNGERI MIFSGEVHPF RLPVPSLWLD VFQKIKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGEYR AEGNFALEPF FDVAKQAGIY LLARPGPYIN AEASGGGFPG
160 170 180 190 200
WLQRVNGTLR TSDPAYLKAT DNYIAHVAAT IAKGQITNGG PVILYQPENE
210 220 230 240 250
YSGACCDATF PDGDYMQYVI DQARNAGIVV PLINNDAWTG GHNAPGTGKG
260 270 280 290 300
EVDIYGHDSY PLGFDCGHPS VWPKGNLPTT FRTDHLKQSP TTPYSLIEFQ
310 320 330 340 350
AGSFDPWGGP GFAACAALVN HEFERVFYKN DLSFGAAILN LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPLTE SRNVTREKYS ELKLIGNFVK ASPSYLLATP
410 420 430 440 450
GNLTTSGYAD TADLTVTPLL GNGTGSYFVV RHTDYTSQAS TPYKLSLPTS
460 470 480 490 500
AGRLTVPQLG GTLTLNGRDS KIHVVDYNVA GTNIIYSTAE VFTWKNFGDS
510 520 530 540 550
KVLILYGGPG EHHELAVSLK SDVQVVEGSN SEFKSKKVGD VVVVAWDVSP
560 570 580 590 600
SRRIVQIGDL KIFLLDRNSV YNYWVPQLDK DDSSTGYSSE KTTASSIIVK
610 620 630 640 650
AGYLVRTAYT KGSGLYLTAD FNATTPVEVI GAPSNVRNLY INGEKTQFKT
660 670 680 690 700
DKNGIWSTEV KYSAPKIKLP SMKDLDWKYL DTLQEVQSTY DDSAWPAADL
710 720 730 740 750
DTTPNTLRPL TTPKSLYSSD YGFHTGYLIY RGHFVADGSE TTFDVRTQGG
760 770 780 790 800
SAFGSSVWLN ESFLGSWTGL NANADYNSTY KLPQVEQGKN YVLTILIDTM
810 820 830 840 850
GLNENWVVGT DEMKNPRGIL SYKLSGRDAS AITWKLTGNL GGEDYQDKIR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPQP PSQKWKSASP LDGLSKPGIG FYTAQFDLDI
910 920 930 940 950
PSGWDVPLYF NFGNSTKSAY RVQLYVNGYQ YGKFVSNIGP QTSFPVPQGI
960 970 980 990 1000
LNYQGTNWVA LTLWALESDG AKLDDFELVN TTPVMTALSK IRPSKQPNYR

QRKGAY
Length:1,006
Mass (Da):110,399
Last modified:July 13, 2010 - v2
Checksum:i9ECBFA8B0F102FBB
GO

Sequence cautioni

The sequence EAL90749 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000004 Genomic DNA Translation: EAL90749.1 Sequence problems.
RefSeqiXP_752787.1, XM_747694.1

Genome annotation databases

EnsemblFungiiCADAFUAT00007691; CADAFUAP00007691; CADAFUAG00007691
GeneIDi3509797
KEGGiafm:AFUA_1G14170

Similar proteinsi

Entry informationi

Entry nameiBGALA_ASPFU
AccessioniPrimary (citable) accession number: Q4WS33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: May 23, 2018
This is version 81 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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