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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei199 – 1991SubstrateBy similarity
Active sitei200 – 2001Proton donorSequence Analysis
Binding sitei260 – 2601SubstrateBy similarity
Active sitei298 – 2981NucleophileSequence Analysis
Binding sitei364 – 3641SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:AFUA_1G14170
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:Afu1g14170.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 1006988Probable beta-galactosidase APRO_0000395217Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi760 – 7601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi777 – 7771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ4WS33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ4WS33.
KOiK01190.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WS33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSVCAIA LLAAQAAGAS IKHMLNGFTL MEHSDPAKRE LLQKYVTWDE
60 70 80 90 100
KSLFVNGERI MIFSGEVHPF RLPVPSLWLD VFQKIKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGEYR AEGNFALEPF FDVAKQAGIY LLARPGPYIN AEASGGGFPG
160 170 180 190 200
WLQRVNGTLR TSDPAYLKAT DNYIAHVAAT IAKGQITNGG PVILYQPENE
210 220 230 240 250
YSGACCDATF PDGDYMQYVI DQARNAGIVV PLINNDAWTG GHNAPGTGKG
260 270 280 290 300
EVDIYGHDSY PLGFDCGHPS VWPKGNLPTT FRTDHLKQSP TTPYSLIEFQ
310 320 330 340 350
AGSFDPWGGP GFAACAALVN HEFERVFYKN DLSFGAAILN LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPLTE SRNVTREKYS ELKLIGNFVK ASPSYLLATP
410 420 430 440 450
GNLTTSGYAD TADLTVTPLL GNGTGSYFVV RHTDYTSQAS TPYKLSLPTS
460 470 480 490 500
AGRLTVPQLG GTLTLNGRDS KIHVVDYNVA GTNIIYSTAE VFTWKNFGDS
510 520 530 540 550
KVLILYGGPG EHHELAVSLK SDVQVVEGSN SEFKSKKVGD VVVVAWDVSP
560 570 580 590 600
SRRIVQIGDL KIFLLDRNSV YNYWVPQLDK DDSSTGYSSE KTTASSIIVK
610 620 630 640 650
AGYLVRTAYT KGSGLYLTAD FNATTPVEVI GAPSNVRNLY INGEKTQFKT
660 670 680 690 700
DKNGIWSTEV KYSAPKIKLP SMKDLDWKYL DTLQEVQSTY DDSAWPAADL
710 720 730 740 750
DTTPNTLRPL TTPKSLYSSD YGFHTGYLIY RGHFVADGSE TTFDVRTQGG
760 770 780 790 800
SAFGSSVWLN ESFLGSWTGL NANADYNSTY KLPQVEQGKN YVLTILIDTM
810 820 830 840 850
GLNENWVVGT DEMKNPRGIL SYKLSGRDAS AITWKLTGNL GGEDYQDKIR
860 870 880 890 900
GPLNEGGLYA ERQGFHQPQP PSQKWKSASP LDGLSKPGIG FYTAQFDLDI
910 920 930 940 950
PSGWDVPLYF NFGNSTKSAY RVQLYVNGYQ YGKFVSNIGP QTSFPVPQGI
960 970 980 990 1000
LNYQGTNWVA LTLWALESDG AKLDDFELVN TTPVMTALSK IRPSKQPNYR

QRKGAY
Length:1,006
Mass (Da):110,399
Last modified:July 13, 2010 - v2
Checksum:i9ECBFA8B0F102FBB
GO

Sequence cautioni

The sequence EAL90749.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000004 Genomic DNA. Translation: EAL90749.1. Sequence problems.
RefSeqiXP_752787.1. XM_747694.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00007691; CADAFUAP00007691; CADAFUAG00007691.
GeneIDi3509797.
KEGGiafm:AFUA_1G14170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000004 Genomic DNA. Translation: EAL90749.1. Sequence problems.
RefSeqiXP_752787.1. XM_747694.1.

3D structure databases

ProteinModelPortaliQ4WS33.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00007691; CADAFUAP00007691; CADAFUAG00007691.
GeneIDi3509797.
KEGGiafm:AFUA_1G14170.

Organism-specific databases

EuPathDBiFungiDB:Afu1g14170.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ4WS33.
KOiK01190.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiBGALA_ASPFU
AccessioniPrimary (citable) accession number: Q4WS33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: June 24, 2015
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.