Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B

Gene

mns1B

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Ca(2+). Can also use Mg2+, but with lower efficiency.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei367 – 3671Proton donorBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. fibrinogen binding Source: ASPGD
  3. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. asexual sporulation resulting in formation of a cellular spore Source: ASPGD
  2. protein N-linked glycosylation Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113)
Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B
Gene namesi
Name:mns1B
Synonyms:msdS
ORF Names:AFUA_1G14560
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle lumen Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Protein family/group databases

Allergomei8990. Asp f Mannosidase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 493475Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BPRO_0000394819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi324 ↔ 353By similarity
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi5085.CADAFUAP00007667.

Structurei

3D structure databases

ProteinModelPortaliQ4WRZ5.
SMRiQ4WRZ5. Positions 31-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ4WRZ5.
KOiK01230.
OMAiGPESFGW.
OrthoDBiEOG7BP8BH.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WRZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLPSLSVAL ALVSSSLALP QAVLPENDVS SRAAAVKEAF SHAWDGYMKY
60 70 80 90 100
AFPHDELLPV SNSYGDSRNG WGASAVDALS TAIVMRNATI VSQILDHIAK
110 120 130 140 150
IDYSKTSDMV SLFETTIRYL GGMLSGYDLL KGPAADLVED RTKVDMLLQQ
160 170 180 190 200
SKNLGDVLKF AFDTPSGVPY NNINITSHGN DGATTNGLAV TGTLVLEWTR
210 220 230 240 250
LSDLTGDQEY AKLSQRAESY LLAPQPSSGE PFPGLVGSAI SIQTGQFTNG
260 270 280 290 300
FVSWNGGSDS FYEYLMKMYV YDPKRFATYK DRWVAAAESS IDHLASNPAS
310 320 330 340 350
RPDLTFLATY NKGSLGLSSQ HLACFDGGSY LLGGTVLDRA DLIDFGLKLV
360 370 380 390 400
DGCAETYHQT LTGIGPESFG WDEKSVPADQ KELYERAGFY VQSGAYILRP
410 420 430 440 450
EVIESFYYAY RVTGKKQYRD WVWNAFENIN KYCRTESGFA GLTNVNAVNG
460 470 480 490
GGRYDNQESF LFAEVMKYAY LTHAPGMSSM PAAAEDKANK SRG
Length:493
Mass (Da):53,840
Last modified:July 5, 2005 - v1
Checksum:iAB055CB6591E76CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000004 Genomic DNA. Translation: EAL90787.1.
RefSeqiXP_752825.1. XM_747732.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00007667; CADAFUAP00007667; CADAFUAG00007667.
GeneIDi3509847.
KEGGiafm:AFUA_1G14560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000004 Genomic DNA. Translation: EAL90787.1.
RefSeqiXP_752825.1. XM_747732.1.

3D structure databases

ProteinModelPortaliQ4WRZ5.
SMRiQ4WRZ5. Positions 31-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5085.CADAFUAP00007667.

Protein family/group databases

Allergomei8990. Asp f Mannosidase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00007667; CADAFUAP00007667; CADAFUAG00007667.
GeneIDi3509847.
KEGGiafm:AFUA_1G14560.

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ4WRZ5.
KOiK01230.
OMAiGPESFGW.
OrthoDBiEOG7BP8BH.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiMNS1B_ASPFU
AccessioniPrimary (citable) accession number: Q4WRZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 5, 2005
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.