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Q4WRD3 (BGALB_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable beta-galactosidase B

EC=3.2.1.23
Alternative name(s):
Lactase B
Gene names
Name:lacB
ORF Names:AFUA_1G16700
OrganismNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]
Taxonomic identifier330879 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1015 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 1015995Probable beta-galactosidase B
PRO_0000395225

Sites

Active site1961Proton donor Potential
Active site3081Nucleophile Potential
Binding site901Substrate By similarity
Binding site1351Substrate By similarity
Binding site1361Substrate; via amide nitrogen By similarity
Binding site1371Substrate By similarity
Binding site1951Substrate By similarity
Binding site2651Substrate By similarity
Binding site3731Substrate By similarity

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1001N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation2111N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential
Glycosylation6791N-linked (GlcNAc...) Potential
Glycosylation7351N-linked (GlcNAc...) Potential
Glycosylation7751N-linked (GlcNAc...) Potential
Glycosylation8211N-linked (GlcNAc...) Potential
Disulfide bond271 ↔ 324 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4WRD3 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: D9EB8677F99A13A8

FASTA1,015111,681
        10         20         30         40         50         60 
MAHIYRLLLL LLSNLWFSTA AQNQSETEWP LHDNGLSKVV QWDHYSFQVN GQRIFIFSGE 

        70         80         90        100        110        120 
FHYWRIPVPE LWRDILEKVK ATGFTAFAFY SSWAYHAPNN STVDFSTGAR DITPIFELAK 

       130        140        150        160        170        180 
ELGMYMIVRP GPYVNAEASA GGFPLWLMTG EYGSLRNDDP RYTAAWTPYF ANMSQITSKY 

       190        200        210        220        230        240 
QVTDGHNTLV YQIENEYGQQ WIGDPKNRNP NKTAVAYMEL LEASARENGI TVPLTSNDPN 

       250        260        270        280        290        300 
MNSKSWGSDW SNAGGNVDVA GLDSYPSCWT CDVSQCTSTN GEYVPYKVID YYDYFQEVQP 

       310        320        330        340        350        360 
TLPSFMPEFQ GGSYNPWAGP EGGCPQDTSA EFANLFYRWN IGQRVTAMSL YMLYGGTNWG 

       370        380        390        400        410        420 
AIAAPVTATS YDYSAPISED RSIGAKYSET KLLALFTRTA KDLTMTEAIG NGTQYTTNTA 

       430        440        450        460        470        480 
VRAFELRNPQ TNAGFYVTFH TDTTVGGNQA FKLHVNTSVG ALTVPKNEGL IQLNGHQSKI 

       490        500        510        520        530        540 
IVTDFTLGKR TLLYSTAEVL TYAVFENRPT LVLWVPTGES GEFAIKGAKS GKVENGDGCS 

       550        560        570        580        590        600 
GIKFKREKDY LVVNFSQAKG LSVLRLDNGV RVVLLDKAAA YRFWAPALTD DPNVQETETV 

       610        620        630        640        650        660 
LVHGPYLVRS ASISKTTLAL RGDSVEKTTL EIFAPHSVRK ITWNGKEVQT SHTPYGSLKA 

       670        680        690        700        710        720 
TLAAPPDIKL PALTSWRSND SLPERLPSYD DSGPAWIEAN HMTTSNPSPP ATFPVLYADE 

       730        740        750        760        770        780 
YGFHNGVRLW RGYFNGSASG VFLNIQGGSA FGWSAWLNGH FLDSHLGTAT TSQANKTLTF 

       790        800        810        820        830        840 
PSSILNPTEN VLLIVHDDTG HDQTTGALNP RGILEARLLS NDTSSPPPEF THWRLAGTAG 

       850        860        870        880        890        900 
GESNLDPIRG VFNEDGLFAE RMGWHLPGFD DSAWTSENSA TSASSALSFT GATVRFFRSV 

       910        920        930        940        950        960 
VPLNIPAGLD VSISFVLSTP TAAPKGYRAQ LFVNGYQYGR YNPHIGNQVV FPVPPGILDY 

       970        980        990       1000       1010 
QGDNTIGLAV WAQTEEGAGI QVDWKVNYVA DSSLSVAGFG KGLRPGWTEE RLKFA 

« Hide

References

[1]"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. expand/collapse author list , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAHF01000004 Genomic DNA. Translation: EAL90999.1.
RefSeqXP_753037.1. XM_747944.1.

3D structure databases

ProteinModelPortalQ4WRD3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5085.CADAFUAP00007142.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFUAT00007142; CADAFUAP00007142; CADAFUAG00007142.
GeneID3510063.
KEGGafm:AFUA_1G16700.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000181922.
KOK01190.
OMANPVLYVD.
OrthoDBEOG7ZGXBD.

Family and domain databases

Gene3D2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SMARTSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGALB_ASPFU
AccessionPrimary (citable) accession number: Q4WRD3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries