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Protein

Protostadienol synthase A

Gene

pdsA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei240 – 2401Proton acceptorBy similarity
Active sitei463 – 4631Proton donorBy similarity

GO - Molecular functioni

  1. lanosterol synthase activity Source: ASPGD

GO - Biological processi

  1. (17Z)-protosta-17(20),24-dien-3beta-ol biosynthetic process Source: ASPGD
  2. pathogenesis Source: UniProtKB-KW
  3. secondary metabolic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Virulence

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16527.

Names & Taxonomyi

Protein namesi
Recommended name:
Protostadienol synthase A (EC:5.4.99.32)
Gene namesi
Name:pdsA
ORF Names:AFUA_4G14770
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g14770.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Protostadienol synthase APRO_0000415495Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUAP00008356.

Structurei

3D structure databases

ProteinModelPortaliQ4WR16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati132 – 17342PFTB 1Add
BLAST
Repeati490 – 53142PFTB 2Add
BLAST
Repeati567 – 60741PFTB 3Add
BLAST
Repeati616 – 66348PFTB 4Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000234317.
InParanoidiQ4WR16.
KOiK15811.
OMAiEREYTEC.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4WR16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDSSMPGT VIGKAEFSDT KAASEFGTDL SRWRLNVDNG RHMWEYLESE
60 70 80 90 100
DEARKRPQSF LEKYWLGLPY ELPARPRATC ALEAVENGWE FFKRLQTADG
110 120 130 140 150
HWGCNDDGPL FVTSGMVIAR YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG
160 170 180 190 200
LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA MAKARNTLHR LGSARATPTW
210 220 230 240 250
GKFWLCVLGV YEWEGMVPLP PEPLLVPASL PFNPGKWWVH TRNVYISMSY
260 270 280 290 300
LYGHRFSMPP NKLVQALRDE LYDIPYQQIN WPAQRTNVSA ADRLTDPTWI
310 320 330 340 350
QRSFTSALTM YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS
360 370 380 390 400
NMLALYHAHG RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT
410 420 430 440 450
VQATIDAGLA ARPENQAILR KALEFIDNSQ IREDPLGVHH VYRQPTRGAW
460 470 480 490 500
PFSTRDQSYA VSDTTAEAVK VIVLLQRIEG FPSRISDERL QQAIDLILGM
510 520 530 540 550
ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC TSSVIMCLTT
560 570 580 590 600
FAREYPTYRP RDIQACLSRS IDYLLRSQYP NGGWFASWGV CFTYATMFAL
610 620 630 640 650
QGLACMGWNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD
660 670 680 690 700
GSQVTNTAYA VIGLLAARCG NHEAIRRGVA YLVKEQQDTG EWLPGPLEGV
710 720 730
FAPPGGMRYP NYKFHFTLMA LGRYVAIHGN ECLAI
Length:735
Mass (Da):83,091
Last modified:July 5, 2005 - v1
Checksum:iAA0AC92A8A6E36F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89318.1.
RefSeqiXP_751356.1. XM_746263.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008356; CADAFUAP00008356; CADAFUAG00008356.
GeneIDi3509337.
KEGGiafm:AFUA_4G14770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89318.1.
RefSeqiXP_751356.1. XM_746263.1.

3D structure databases

ProteinModelPortaliQ4WR16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5085.CADAFUAP00008356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00008356; CADAFUAP00008356; CADAFUAG00008356.
GeneIDi3509337.
KEGGiafm:AFUA_4G14770.

Organism-specific databases

EuPathDBiFungiDB:Afu4g14770.

Phylogenomic databases

HOGENOMiHOG000234317.
InParanoidiQ4WR16.
KOiK15811.
OMAiEREYTEC.
OrthoDBiEOG70610B.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16527.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  2. "Protostadienol biosynthesis and metabolism in the pathogenic fungus Aspergillus fumigatus."
    Lodeiro S., Xiong Q., Wilson W.K., Ivanova Y., Smith M.L., May G.S., Matsuda S.P.
    Org. Lett. 11:1241-1244(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPDSA_ASPFU
AccessioniPrimary (citable) accession number: Q4WR16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2005
Last modified: April 29, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.