SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q4WR16

- PDSA_ASPFU

UniProt

Q4WR16 - PDSA_ASPFU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protostadienol synthase A
Gene
pdsA, AFUA_4G14770
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei240 – 2401Proton acceptor By similarity
Active sitei463 – 4631Proton donor By similarity

GO - Molecular functioni

  1. lanosterol synthase activity Source: ASPGD
Complete GO annotation...

GO - Biological processi

  1. (17Z)-protosta-17(20),24-dien-3beta-ol biosynthetic process Source: ASPGD
  2. pathogenesis Source: UniProtKB-KW
  3. secondary metabolic process Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Virulence

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16527.

Names & Taxonomyi

Protein namesi
Recommended name:
Protostadienol synthase A (EC:5.4.99.32)
Gene namesi
Name:pdsA
ORF Names:AFUA_4G14770
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530: Chromosome 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Protostadienol synthase A
PRO_0000415495Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUAP00008356.

Structurei

3D structure databases

ProteinModelPortaliQ4WR16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati132 – 17342PFTB 1
Add
BLAST
Repeati490 – 53142PFTB 2
Add
BLAST
Repeati567 – 60741PFTB 3
Add
BLAST
Repeati616 – 66348PFTB 4
Add
BLAST

Sequence similaritiesi

Contains 4 PFTB repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000234317.
KOiK15811.
OMAiETRNTHY.
OrthoDBiEOG70610B.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4WR16-1 [UniParc]FASTAAdd to Basket

« Hide

MATDSSMPGT VIGKAEFSDT KAASEFGTDL SRWRLNVDNG RHMWEYLESE    50
DEARKRPQSF LEKYWLGLPY ELPARPRATC ALEAVENGWE FFKRLQTADG 100
HWGCNDDGPL FVTSGMVIAR YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG 150
LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA MAKARNTLHR LGSARATPTW 200
GKFWLCVLGV YEWEGMVPLP PEPLLVPASL PFNPGKWWVH TRNVYISMSY 250
LYGHRFSMPP NKLVQALRDE LYDIPYQQIN WPAQRTNVSA ADRLTDPTWI 300
QRSFTSALTM YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS 350
NMLALYHAHG RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT 400
VQATIDAGLA ARPENQAILR KALEFIDNSQ IREDPLGVHH VYRQPTRGAW 450
PFSTRDQSYA VSDTTAEAVK VIVLLQRIEG FPSRISDERL QQAIDLILGM 500
ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC TSSVIMCLTT 550
FAREYPTYRP RDIQACLSRS IDYLLRSQYP NGGWFASWGV CFTYATMFAL 600
QGLACMGWNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD 650
GSQVTNTAYA VIGLLAARCG NHEAIRRGVA YLVKEQQDTG EWLPGPLEGV 700
FAPPGGMRYP NYKFHFTLMA LGRYVAIHGN ECLAI 735
Length:735
Mass (Da):83,091
Last modified:July 5, 2005 - v1
Checksum:iAA0AC92A8A6E36F8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89318.1.
RefSeqiXP_751356.1. XM_746263.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008356; CADAFUAP00008356; CADAFUAG00008356.
GeneIDi3509337.
KEGGiafm:AFUA_4G14770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89318.1 .
RefSeqi XP_751356.1. XM_746263.1.

3D structure databases

ProteinModelPortali Q4WR16.
ModBasei Search...

Protein-protein interaction databases

STRINGi 5085.CADAFUAP00008356.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFUAT00008356 ; CADAFUAP00008356 ; CADAFUAG00008356 .
GeneIDi 3509337.
KEGGi afm:AFUA_4G14770.

Phylogenomic databases

HOGENOMi HOG000234317.
KOi K15811.
OMAi ETRNTHY.
OrthoDBi EOG70610B.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16527.

Family and domain databases

Gene3Di 1.50.10.20. 2 hits.
InterProi IPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00432. Prenyltrans. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 2 hits.
TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  2. "Protostadienol biosynthesis and metabolism in the pathogenic fungus Aspergillus fumigatus."
    Lodeiro S., Xiong Q., Wilson W.K., Ivanova Y., Smith M.L., May G.S., Matsuda S.P.
    Org. Lett. 11:1241-1244(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPDSA_ASPFU
AccessioniPrimary (citable) accession number: Q4WR16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi