Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q4WR16

- PDSA_ASPFU

UniProt

Q4WR16 - PDSA_ASPFU

Protein

Protostadienol synthase A

Gene

pdsA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence.1 Publication

    Catalytic activityi

    (3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei240 – 2401Proton acceptorBy similarity
    Active sitei463 – 4631Proton donorBy similarity

    GO - Molecular functioni

    1. lanosterol synthase activity Source: ASPGD

    GO - Biological processi

    1. (17Z)-protosta-17(20),24-dien-3beta-ol biosynthetic process Source: ASPGD
    2. pathogenesis Source: UniProtKB-KW
    3. secondary metabolic process Source: ASPGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Virulence

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protostadienol synthase A (EC:5.4.99.32)
    Gene namesi
    Name:pdsA
    ORF Names:AFUA_4G14770
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000002530: Chromosome 4

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Protostadienol synthase APRO_0000415495Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5085.CADAFUAP00008356.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4WR16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati132 – 17342PFTB 1Add
    BLAST
    Repeati490 – 53142PFTB 2Add
    BLAST
    Repeati567 – 60741PFTB 3Add
    BLAST
    Repeati616 – 66348PFTB 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the terpene cyclase/mutase family.Curated
    Contains 4 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOGENOMiHOG000234317.
    KOiK15811.
    OMAiETRNTHY.
    OrthoDBiEOG70610B.

    Family and domain databases

    Gene3Di1.50.10.20. 2 hits.
    InterProiIPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF00432. Prenyltrans. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4WR16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATDSSMPGT VIGKAEFSDT KAASEFGTDL SRWRLNVDNG RHMWEYLESE    50
    DEARKRPQSF LEKYWLGLPY ELPARPRATC ALEAVENGWE FFKRLQTADG 100
    HWGCNDDGPL FVTSGMVIAR YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG 150
    LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA MAKARNTLHR LGSARATPTW 200
    GKFWLCVLGV YEWEGMVPLP PEPLLVPASL PFNPGKWWVH TRNVYISMSY 250
    LYGHRFSMPP NKLVQALRDE LYDIPYQQIN WPAQRTNVSA ADRLTDPTWI 300
    QRSFTSALTM YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS 350
    NMLALYHAHG RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT 400
    VQATIDAGLA ARPENQAILR KALEFIDNSQ IREDPLGVHH VYRQPTRGAW 450
    PFSTRDQSYA VSDTTAEAVK VIVLLQRIEG FPSRISDERL QQAIDLILGM 500
    ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC TSSVIMCLTT 550
    FAREYPTYRP RDIQACLSRS IDYLLRSQYP NGGWFASWGV CFTYATMFAL 600
    QGLACMGWNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD 650
    GSQVTNTAYA VIGLLAARCG NHEAIRRGVA YLVKEQQDTG EWLPGPLEGV 700
    FAPPGGMRYP NYKFHFTLMA LGRYVAIHGN ECLAI 735
    Length:735
    Mass (Da):83,091
    Last modified:July 5, 2005 - v1
    Checksum:iAA0AC92A8A6E36F8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000005 Genomic DNA. Translation: EAL89318.1.
    RefSeqiXP_751356.1. XM_746263.1.

    Genome annotation databases

    EnsemblFungiiCADAFUAT00008356; CADAFUAP00008356; CADAFUAG00008356.
    GeneIDi3509337.
    KEGGiafm:AFUA_4G14770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000005 Genomic DNA. Translation: EAL89318.1 .
    RefSeqi XP_751356.1. XM_746263.1.

    3D structure databases

    ProteinModelPortali Q4WR16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5085.CADAFUAP00008356.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFUAT00008356 ; CADAFUAP00008356 ; CADAFUAG00008356 .
    GeneIDi 3509337.
    KEGGi afm:AFUA_4G14770.

    Phylogenomic databases

    HOGENOMi HOG000234317.
    KOi K15811.
    OMAi ETRNTHY.
    OrthoDBi EOG70610B.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16527.

    Family and domain databases

    Gene3Di 1.50.10.20. 2 hits.
    InterProi IPR001330. Prenyltrans.
    IPR018333. Squalene_cyclase.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view ]
    Pfami PF00432. Prenyltrans. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 2 hits.
    TIGRFAMsi TIGR01787. squalene_cyclas. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
      Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
      , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
      Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
    2. "Protostadienol biosynthesis and metabolism in the pathogenic fungus Aspergillus fumigatus."
      Lodeiro S., Xiong Q., Wilson W.K., Ivanova Y., Smith M.L., May G.S., Matsuda S.P.
      Org. Lett. 11:1241-1244(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.

    Entry informationi

    Entry nameiPDSA_ASPFU
    AccessioniPrimary (citable) accession number: Q4WR16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3