Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4WR16 (PDSA_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protostadienol synthase A

EC=5.4.99.32
Gene names
Name:pdsA
ORF Names:AFUA_4G14770
OrganismNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]
Taxonomic identifier330879 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protostadienol synthase which cyclizes (3S)-oxidosqualene to (17Z)-protosta-17(20),24-dien-3-beta-ol (protostadienol), the biosynthetic precursor of helvolic acid, a secundary metabolite which promotes virulence. Ref.2

Catalytic activity

(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3-beta-ol. Ref.2

Sequence similarities

Belongs to the terpene cyclase/mutase family.

Contains 4 PFTB repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Protostadienol synthase A
PRO_0000415495

Regions

Repeat132 – 17342PFTB 1
Repeat490 – 53142PFTB 2
Repeat567 – 60741PFTB 3
Repeat616 – 66348PFTB 4

Sites

Active site2401Proton acceptor By similarity
Active site4631Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4WR16 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: AA0AC92A8A6E36F8

FASTA73583,091
        10         20         30         40         50         60 
MATDSSMPGT VIGKAEFSDT KAASEFGTDL SRWRLNVDNG RHMWEYLESE DEARKRPQSF 

        70         80         90        100        110        120 
LEKYWLGLPY ELPARPRATC ALEAVENGWE FFKRLQTADG HWGCNDDGPL FVTSGMVIAR 

       130        140        150        160        170        180 
YIVGIPIDSH MKQEMCRYLL NVVNEDGGWG LFIQSPSTVF GTVMNYCMLR ILGLGPEHPA 

       190        200        210        220        230        240 
MAKARNTLHR LGSARATPTW GKFWLCVLGV YEWEGMVPLP PEPLLVPASL PFNPGKWWVH 

       250        260        270        280        290        300 
TRNVYISMSY LYGHRFSMPP NKLVQALRDE LYDIPYQQIN WPAQRTNVSA ADRLTDPTWI 

       310        320        330        340        350        360 
QRSFTSALTM YETFKIPFLR RRALNEALFQ IETETRNTHY LCIAPVSFAS NMLALYHAHG 

       370        380        390        400        410        420 
RDSHWIRGMR DRFIDPMWLC REGLAASGTN GTSLWDTALT VQATIDAGLA ARPENQAILR 

       430        440        450        460        470        480 
KALEFIDNSQ IREDPLGVHH VYRQPTRGAW PFSTRDQSYA VSDTTAEAVK VIVLLQRIEG 

       490        500        510        520        530        540 
FPSRISDERL QQAIDLILGM ENAGGGFSAY EPVRGPKFLE LLNITELYEN VMTDNLYPEC 

       550        560        570        580        590        600 
TSSVIMCLTT FAREYPTYRP RDIQACLSRS IDYLLRSQYP NGGWFASWGV CFTYATMFAL 

       610        620        630        640        650        660 
QGLACMGWNE SNCAACQRAC SFLLQHQNPD GGWGESLDTV RFKQYLPHPD GSQVTNTAYA 

       670        680        690        700        710        720 
VIGLLAARCG NHEAIRRGVA YLVKEQQDTG EWLPGPLEGV FAPPGGMRYP NYKFHFTLMA 

       730 
LGRYVAIHGN ECLAI 

« Hide

References

« Hide 'large scale' references
[1]"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. expand/collapse author list , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[2]"Protostadienol biosynthesis and metabolism in the pathogenic fungus Aspergillus fumigatus."
Lodeiro S., Xiong Q., Wilson W.K., Ivanova Y., Smith M.L., May G.S., Matsuda S.P.
Org. Lett. 11:1241-1244(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAHF01000005 Genomic DNA. Translation: EAL89318.1.
RefSeqXP_751356.1. XM_746263.1.

3D structure databases

ProteinModelPortalQ4WR16.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5085.CADAFUAP00008356.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFUAT00008356; CADAFUAP00008356; CADAFUAG00008356.
GeneID3509337.
KEGGafm:AFUA_4G14770.

Phylogenomic databases

HOGENOMHOG000234317.
KOK15811.
OMAETRNTHY.
OrthoDBEOG70610B.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16527.

Family and domain databases

Gene3D1.50.10.20. 2 hits.
InterProIPR001330. Prenyltrans.
IPR018333. Squalene_cyclase.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamPF00432. Prenyltrans. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 2 hits.
TIGRFAMsTIGR01787. squalene_cyclas. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDSA_ASPFU
AccessionPrimary (citable) accession number: Q4WR16
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families