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Protein

Glutathione S-transferase-like protein tpcF

Gene

tpcF

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glutathione S-transferase-like protein; part of the gene cluster that mediates the biosynthesis of trypacidin, a mycotoxin with antiprotozoal activity and that plays a role in the infection process (PubMed:26278536, PubMed:26242966). The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) tpcC (PubMed:26242966). The atrochrysone carboxyl ACP thioesterase tpcB then breaks the thioester bond and releases the atrochrysone carboxylic acid from tpcC (PubMed:26242966). The decarboxylase tpcK converts atrochrysone carboxylic acid to atrochrysone which is further reduced into emodin anthrone (PubMed:26242966). The next step is performed by the emodin anthrone oxygenase tpcL that catalyzes the oxidation of emodinanthrone to emodin (PubMed:26242966). Emodin O-methyltransferase encoded by tpcA catalyzes methylation of the 8-hydroxy group of emodin to form questin (PubMed:26242966). Ring cleavage of questin by questin oxidase tpcI leads to desmethylsulochrin via several intermediates including questin epoxide (By similarity). Another methylation step catalyzed by tpcM leads to the formation of sulochrin which is further converted to monomethylsulfochrin by tpcH. Finally, the tpcJ catalyzes the conversion of monomethylsulfochrin to trypacidin (PubMed:26242966). Trypacidin is toxic for human pulmonary and bronchial epithelial cells by initiating the intracellular formation of nitric oxide (NO) and hydrogen peroxide (H2O2), thus triggering host necrotic cell death (PubMed:22319557). The trypacidin pathway is also able to produce endocrocin via a distinct route from the endocrocin Enc pathway (PubMed:26242966).By similarity3 Publications

Pathwayi: Secondary metabolite biosynthesis

This protein is involved in Secondary metabolite biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in Secondary metabolite biosynthesis.

GO - Molecular functioni

  • glutathione peroxidase activity Source: ASPGD
  • glutathione transferase activity Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase-like protein tpcF1 Publication (EC:2.5.1.-1 Publication)
Alternative name(s):
Trypacidin synthesis protein E1 Publication
Gene namesi
Name:tpcF1 Publication
Synonyms:tynF1 Publication
ORF Names:AFUA_4G14530
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g14530.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: ASPGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Glutathione S-transferase-like protein tpcFPRO_0000437069Add
BLAST

Proteomic databases

PRIDEiQ4WQZ2.

Expressioni

Tissue specificityi

Specifically expressed in conidia (PubMed:22319557).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ4WQZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8582GST N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini92 – 225134GST C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GST superfamily.Curated
Contains 1 GST C-terminal domain.PROSITE-ProRule annotation
Contains 1 GST N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000125752.
InParanoidiQ4WQZ2.
KOiK00799.
OMAiHTAEPRI.
OrthoDBiEOG092C499E.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WQZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDIQPITVY GKGGPNPPRV AIILAELDLP HKVIEVPLSK VKEPDYVAIN
60 70 80 90 100
PNGRIPAIYD PNTDLTLWES GAIVEYLVSH YDPDHRISFP AGSNLAALAT
110 120 130 140 150
QWLFFQASGQ GPYYGQASWF KKFHHEKVPS AIERYVKEIN RVTGVLEGHL
160 170 180 190 200
SRQKVAADGD GPWLVGGKCS FADLAWIPWQ VIVTAIIQPE DGYTVEDYPH
210 220
VKNWLDRMMA RPGVQKGMAD IFPST
Length:225
Mass (Da):25,031
Last modified:July 5, 2005 - v1
Checksum:iB2AEBF68D76148B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89342.1.
RefSeqiXP_751380.1. XM_746287.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008190; CADAFUAP00008190; CADAFUAG00008190.
GeneIDi3509604.
KEGGiafm:AFUA_4G14530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89342.1.
RefSeqiXP_751380.1. XM_746287.1.

3D structure databases

ProteinModelPortaliQ4WQZ2.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ4WQZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00008190; CADAFUAP00008190; CADAFUAG00008190.
GeneIDi3509604.
KEGGiafm:AFUA_4G14530.

Organism-specific databases

EuPathDBiFungiDB:Afu4g14530.

Phylogenomic databases

HOGENOMiHOG000125752.
InParanoidiQ4WQZ2.
KOiK00799.
OMAiHTAEPRI.
OrthoDBiEOG092C499E.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPCF_ASPFU
AccessioniPrimary (citable) accession number: Q4WQZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.