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Q4WQY4

- M2DH_ASPFU

UniProt

Q4WQY4 - M2DH_ASPFU

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Protein

Mannitol 2-dehydrogenase

Gene

AFUA_4G14450

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the NAD(H)-dependent interconversion of D-fructose and D-mannitol in the mannitol metabolic pathway. Has a preference for NADH over NADPH.1 Publication

Catalytic activityi

D-mannitol + NAD+ = D-fructose + NADH.

Kineticsi

  1. KM=13 mM for D-mannitol (in the presence of NAD+)1 Publication
  2. KM=82 mM for D-mannitol (in the presence of NADP+)1 Publication
  3. KM=60 mM for D-fructose (in the presence of NADH)1 Publication
  4. KM=159 mM for D-fructose (in the presence of NADPH)1 Publication
  5. KM=0.019 mM for NADH1 Publication
  6. KM=0.15 mM for NAD+1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 4812NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. mannitol 2-dehydrogenase activity Source: UniProtKB
  2. NAD binding Source: UniProtKB
  3. NADP binding Source: UniProtKB

GO - Biological processi

  1. mannitol metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol 2-dehydrogenase (EC:1.1.1.67)
Short name:
M2DH
Short name:
MDH
Gene namesi
ORF Names:AFUA_4G14450
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530: Chromosome 4

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Mannitol 2-dehydrogenasePRO_0000371540Add
BLAST

Proteomic databases

PRIDEiQ4WQY4.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi5085.CADAFUAP00008043.

Structurei

3D structure databases

ProteinModelPortaliQ4WQY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mannitol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG0246.
HOGENOMiHOG000202982.
InParanoidiQ4WQY4.
KOiK00045.
OMAiWLHFVRR.
OrthoDBiEOG7PCJRT.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSiPR00084. MTLDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4WQY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPLKLNSRN LSQIAAAGGA LVKIPTYQRG RAVKEGIVHI GVGGFHRAHL
60 70 80 90 100
AVYIDQLMQK HGVNDYAICG VGLQPFDSAM RDALASQDHL YTLIERSAKG
110 120 130 140 150
SFAHVIGSIN SYLFAPDNRE AVIAKMAHPD TKIVSLTITE SGYYYNENTH
160 170 180 190 200
ELQSEHPDIQ FDLDPANEKA PRTTFGFLYA GLTRRYQQGL KPFTVMSCDN
210 220 230 240 250
MQKNGSITRH MLESFARLRN PEVAEWIAEE GAFPNAMVDR ITPQTSETDK
260 270 280 290 300
TALAEKFGIV DSWPVVTEPF TQWVIEDQFS DGRPPFEKVG VQVVKDVHAV
310 320 330 340 350
EQFEKHKLRL LNGSHSALGY PGQLAGFQYV HEVMANPLFR KFVWQMMQEE
360 370 380 390 400
VKPLLPEIPG VDIDEYCNTL IERFTNPTIM DQLPRICLNA SGKIPQFIMP
410 420 430 440 450
SIAEAIWETG PFRRLCFVAA AWFHYIKGVD DRGKPFEVVD PMREELQAKA
460 470 480 490 500
RAGGNDPSEL LSIKSLFGDD LRNDERFLRE ITTAMNDIAR DGIMKTLPKY

IN
Length:502
Mass (Da):56,475
Last modified:July 5, 2005 - v1
Checksum:i230384292D807EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89350.1.
RefSeqiXP_751388.1. XM_746295.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008043; CADAFUAP00008043; CADAFUAG00008043.
GeneIDi3509495.
KEGGiafm:AFUA_4G14450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA. Translation: EAL89350.1 .
RefSeqi XP_751388.1. XM_746295.1.

3D structure databases

ProteinModelPortali Q4WQY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5085.CADAFUAP00008043.

Proteomic databases

PRIDEi Q4WQY4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFUAT00008043 ; CADAFUAP00008043 ; CADAFUAG00008043 .
GeneIDi 3509495.
KEGGi afm:AFUA_4G14450.

Phylogenomic databases

eggNOGi COG0246.
HOGENOMi HOG000202982.
InParanoidi Q4WQY4.
KOi K00045.
OMAi WLHFVRR.
OrthoDBi EOG7PCJRT.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view ]
PRINTSi PR00084. MTLDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  2. "Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase."
    Krahulec S., Armao G.C., Bubner P., Klimacek M., Nidetzky B.
    Chem. Biol. Interact. 178:274-282(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiM2DH_ASPFU
AccessioniPrimary (citable) accession number: Q4WQY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3