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Q4WQY4 (M2DH_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannitol 2-dehydrogenase

Short name=M2DH
Short name=MDH
EC=1.1.1.67
Gene names
ORF Names:AFUA_4G14450
OrganismNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]
Taxonomic identifier330879 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the NAD(H)-dependent interconversion of D-fructose and D-mannitol in the mannitol metabolic pathway. Has a preference for NADH over NADPH. Ref.2

Catalytic activity

D-mannitol + NAD+ = D-fructose + NADH.

Subunit structure

Monomer. Ref.2

Sequence similarities

Belongs to the mannitol dehydrogenase family.

Biophysicochemical properties

Kinetic parameters:

KM=13 mM for D-mannitol (in the presence of NAD+) Ref.2

KM=82 mM for D-mannitol (in the presence of NADP+)

KM=60 mM for D-fructose (in the presence of NADH)

KM=159 mM for D-fructose (in the presence of NADPH)

KM=0.019 mM for NADH

KM=0.15 mM for NAD+

Ontologies

Keywords
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmannitol metabolic process

Inferred by curator Ref.2. Source: UniProtKB

   Molecular_functionNAD binding

Inferred from direct assay Ref.2. Source: UniProtKB

NADP binding

Inferred from direct assay Ref.2. Source: UniProtKB

mannitol 2-dehydrogenase activity

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Mannitol 2-dehydrogenase
PRO_0000371540

Regions

Nucleotide binding37 – 4812NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4WQY4 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 230384292D807EB1

FASTA50256,475
        10         20         30         40         50         60 
MAPLKLNSRN LSQIAAAGGA LVKIPTYQRG RAVKEGIVHI GVGGFHRAHL AVYIDQLMQK 

        70         80         90        100        110        120 
HGVNDYAICG VGLQPFDSAM RDALASQDHL YTLIERSAKG SFAHVIGSIN SYLFAPDNRE 

       130        140        150        160        170        180 
AVIAKMAHPD TKIVSLTITE SGYYYNENTH ELQSEHPDIQ FDLDPANEKA PRTTFGFLYA 

       190        200        210        220        230        240 
GLTRRYQQGL KPFTVMSCDN MQKNGSITRH MLESFARLRN PEVAEWIAEE GAFPNAMVDR 

       250        260        270        280        290        300 
ITPQTSETDK TALAEKFGIV DSWPVVTEPF TQWVIEDQFS DGRPPFEKVG VQVVKDVHAV 

       310        320        330        340        350        360 
EQFEKHKLRL LNGSHSALGY PGQLAGFQYV HEVMANPLFR KFVWQMMQEE VKPLLPEIPG 

       370        380        390        400        410        420 
VDIDEYCNTL IERFTNPTIM DQLPRICLNA SGKIPQFIMP SIAEAIWETG PFRRLCFVAA 

       430        440        450        460        470        480 
AWFHYIKGVD DRGKPFEVVD PMREELQAKA RAGGNDPSEL LSIKSLFGDD LRNDERFLRE 

       490        500 
ITTAMNDIAR DGIMKTLPKY IN 

« Hide

References

« Hide 'large scale' references
[1]"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. expand/collapse author list , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
[2]"Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase."
Krahulec S., Armao G.C., Bubner P., Klimacek M., Nidetzky B.
Chem. Biol. Interact. 178:274-282(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAHF01000005 Genomic DNA. Translation: EAL89350.1.
RefSeqXP_751388.1. XM_746295.1.

3D structure databases

ProteinModelPortalQ4WQY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5085.CADAFUAP00008043.

Proteomic databases

PRIDEQ4WQY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFUAT00008043; CADAFUAP00008043; CADAFUAG00008043.
GeneID3509495.
KEGGafm:AFUA_4G14450.

Phylogenomic databases

eggNOGCOG0246.
HOGENOMHOG000202982.
KOK00045.
OMAFEKVGVQ.
OrthoDBEOG7PCJRT.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSPR00084. MTLDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
ProtoNetSearch...

Entry information

Entry nameM2DH_ASPFU
AccessionPrimary (citable) accession number: Q4WQY4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2005
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families