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Q4WQY4

- M2DH_ASPFU

UniProt

Q4WQY4 - M2DH_ASPFU

Protein

Mannitol 2-dehydrogenase

Gene

AFUA_4G14450

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(H)-dependent interconversion of D-fructose and D-mannitol in the mannitol metabolic pathway. Has a preference for NADH over NADPH.1 Publication

    Catalytic activityi

    D-mannitol + NAD+ = D-fructose + NADH.

    Kineticsi

    1. KM=13 mM for D-mannitol (in the presence of NAD+)1 Publication
    2. KM=82 mM for D-mannitol (in the presence of NADP+)1 Publication
    3. KM=60 mM for D-fructose (in the presence of NADH)1 Publication
    4. KM=159 mM for D-fructose (in the presence of NADPH)1 Publication
    5. KM=0.019 mM for NADH1 Publication
    6. KM=0.15 mM for NAD+1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi37 – 4812NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. mannitol 2-dehydrogenase activity Source: UniProtKB
    2. NAD binding Source: UniProtKB
    3. NADP binding Source: UniProtKB

    GO - Biological processi

    1. mannitol metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannitol 2-dehydrogenase (EC:1.1.1.67)
    Short name:
    M2DH
    Short name:
    MDH
    Gene namesi
    ORF Names:AFUA_4G14450
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000002530: Chromosome 4

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Mannitol 2-dehydrogenasePRO_0000371540Add
    BLAST

    Proteomic databases

    PRIDEiQ4WQY4.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi5085.CADAFUAP00008043.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4WQY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the mannitol dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG0246.
    HOGENOMiHOG000202982.
    KOiK00045.
    OMAiWLHFVRR.
    OrthoDBiEOG7PCJRT.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR000669. Mannitol_DH.
    IPR013118. Mannitol_DH_C.
    IPR013131. Mannitol_DH_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01232. Mannitol_dh. 1 hit.
    PF08125. Mannitol_dh_C. 1 hit.
    [Graphical view]
    PRINTSiPR00084. MTLDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4WQY4-1 [UniParc]FASTAAdd to Basket

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    MAPLKLNSRN LSQIAAAGGA LVKIPTYQRG RAVKEGIVHI GVGGFHRAHL    50
    AVYIDQLMQK HGVNDYAICG VGLQPFDSAM RDALASQDHL YTLIERSAKG 100
    SFAHVIGSIN SYLFAPDNRE AVIAKMAHPD TKIVSLTITE SGYYYNENTH 150
    ELQSEHPDIQ FDLDPANEKA PRTTFGFLYA GLTRRYQQGL KPFTVMSCDN 200
    MQKNGSITRH MLESFARLRN PEVAEWIAEE GAFPNAMVDR ITPQTSETDK 250
    TALAEKFGIV DSWPVVTEPF TQWVIEDQFS DGRPPFEKVG VQVVKDVHAV 300
    EQFEKHKLRL LNGSHSALGY PGQLAGFQYV HEVMANPLFR KFVWQMMQEE 350
    VKPLLPEIPG VDIDEYCNTL IERFTNPTIM DQLPRICLNA SGKIPQFIMP 400
    SIAEAIWETG PFRRLCFVAA AWFHYIKGVD DRGKPFEVVD PMREELQAKA 450
    RAGGNDPSEL LSIKSLFGDD LRNDERFLRE ITTAMNDIAR DGIMKTLPKY 500
    IN 502
    Length:502
    Mass (Da):56,475
    Last modified:July 5, 2005 - v1
    Checksum:i230384292D807EB1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000005 Genomic DNA. Translation: EAL89350.1.
    RefSeqiXP_751388.1. XM_746295.1.

    Genome annotation databases

    EnsemblFungiiCADAFUAT00008043; CADAFUAP00008043; CADAFUAG00008043.
    GeneIDi3509495.
    KEGGiafm:AFUA_4G14450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAHF01000005 Genomic DNA. Translation: EAL89350.1 .
    RefSeqi XP_751388.1. XM_746295.1.

    3D structure databases

    ProteinModelPortali Q4WQY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5085.CADAFUAP00008043.

    Proteomic databases

    PRIDEi Q4WQY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAFUAT00008043 ; CADAFUAP00008043 ; CADAFUAG00008043 .
    GeneIDi 3509495.
    KEGGi afm:AFUA_4G14450.

    Phylogenomic databases

    eggNOGi COG0246.
    HOGENOMi HOG000202982.
    KOi K00045.
    OMAi WLHFVRR.
    OrthoDBi EOG7PCJRT.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR000669. Mannitol_DH.
    IPR013118. Mannitol_DH_C.
    IPR013131. Mannitol_DH_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01232. Mannitol_dh. 1 hit.
    PF08125. Mannitol_dh_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00084. MTLDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
      Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
      , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
      Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
    2. "Polyol-specific long-chain dehydrogenases/reductases of mannitol metabolism in Aspergillus fumigatus: biochemical characterization and pH studies of mannitol 2-dehydrogenase and mannitol-1-phosphate 5-dehydrogenase."
      Krahulec S., Armao G.C., Bubner P., Klimacek M., Nidetzky B.
      Chem. Biol. Interact. 178:274-282(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiM2DH_ASPFU
    AccessioniPrimary (citable) accession number: Q4WQY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3