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Protein

Mannitol 2-dehydrogenase

Gene

AFUA_4G14450

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NAD(H)-dependent interconversion of D-fructose and D-mannitol in the mannitol metabolic pathway. Has a preference for NADH over NADPH.1 Publication

Catalytic activityi

D-mannitol + NAD+ = D-fructose + NADH.

Kineticsi

  1. KM=13 mM for D-mannitol (in the presence of NAD+)1 Publication
  2. KM=82 mM for D-mannitol (in the presence of NADP+)1 Publication
  3. KM=60 mM for D-fructose (in the presence of NADH)1 Publication
  4. KM=159 mM for D-fructose (in the presence of NADPH)1 Publication
  5. KM=0.019 mM for NADH1 Publication
  6. KM=0.15 mM for NAD+1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi37 – 48NADBy similarityAdd BLAST12

    GO - Molecular functioni

    • mannitol 2-dehydrogenase activity Source: UniProtKB
    • NAD binding Source: UniProtKB
    • NADP binding Source: UniProtKB

    GO - Biological processi

    • mannitol metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    LigandNAD, NADP

    Enzyme and pathway databases

    BRENDAi1.1.1.67 508

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannitol 2-dehydrogenase (EC:1.1.1.67)
    Short name:
    M2DH
    Short name:
    MDH
    Gene namesi
    ORF Names:AFUA_4G14450
    OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
    Taxonomic identifieri330879 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    Proteomesi
    • UP000002530 Componentsi: Chromosome 4, Unassembled WGS sequence

    Organism-specific databases

    EuPathDBiFungiDB:Afu4g14450

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003715401 – 502Mannitol 2-dehydrogenaseAdd BLAST502

    Proteomic databases

    PRIDEiQ4WQY4

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi5085.CADAFUBP00006987

    Structurei

    3D structure databases

    ProteinModelPortaliQ4WQY4
    SMRiQ4WQY4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the mannitol dehydrogenase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000202982
    InParanoidiQ4WQY4
    KOiK00045
    OMAiEDSFPNG
    OrthoDBiEOG092C1JOH

    Family and domain databases

    Gene3Di1.10.1040.10, 1 hit
    InterProiView protein in InterPro
    IPR008927 6-PGluconate_DH-like_C_sf
    IPR013328 6PGD_dom2
    IPR000669 Mannitol_DH
    IPR013118 Mannitol_DH_C
    IPR013131 Mannitol_DH_N
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF01232 Mannitol_dh, 1 hit
    PF08125 Mannitol_dh_C, 1 hit
    PRINTSiPR00084 MTLDHDRGNASE
    SUPFAMiSSF48179 SSF48179, 1 hit
    SSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q4WQY4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAPLKLNSRN LSQIAAAGGA LVKIPTYQRG RAVKEGIVHI GVGGFHRAHL
    60 70 80 90 100
    AVYIDQLMQK HGVNDYAICG VGLQPFDSAM RDALASQDHL YTLIERSAKG
    110 120 130 140 150
    SFAHVIGSIN SYLFAPDNRE AVIAKMAHPD TKIVSLTITE SGYYYNENTH
    160 170 180 190 200
    ELQSEHPDIQ FDLDPANEKA PRTTFGFLYA GLTRRYQQGL KPFTVMSCDN
    210 220 230 240 250
    MQKNGSITRH MLESFARLRN PEVAEWIAEE GAFPNAMVDR ITPQTSETDK
    260 270 280 290 300
    TALAEKFGIV DSWPVVTEPF TQWVIEDQFS DGRPPFEKVG VQVVKDVHAV
    310 320 330 340 350
    EQFEKHKLRL LNGSHSALGY PGQLAGFQYV HEVMANPLFR KFVWQMMQEE
    360 370 380 390 400
    VKPLLPEIPG VDIDEYCNTL IERFTNPTIM DQLPRICLNA SGKIPQFIMP
    410 420 430 440 450
    SIAEAIWETG PFRRLCFVAA AWFHYIKGVD DRGKPFEVVD PMREELQAKA
    460 470 480 490 500
    RAGGNDPSEL LSIKSLFGDD LRNDERFLRE ITTAMNDIAR DGIMKTLPKY

    IN
    Length:502
    Mass (Da):56,475
    Last modified:July 5, 2005 - v1
    Checksum:i230384292D807EB1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAHF01000005 Genomic DNA Translation: EAL89350.1
    RefSeqiXP_751388.1, XM_746295.1

    Genome annotation databases

    EnsemblFungiiCADAFUAT00008043; CADAFUAP00008043; CADAFUAG00008043
    GeneIDi3509495
    KEGGiafm:AFUA_4G14450

    Similar proteinsi

    Entry informationi

    Entry nameiM2DH_ASPFU
    AccessioniPrimary (citable) accession number: Q4WQY4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: July 5, 2005
    Last modified: April 25, 2018
    This is version 88 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health