Q4WQB9 (TRMB_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trm8
ORF Names:	AFUA_4G12280

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with trm82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370586
Regions
	Region	122 – 123	2	S-adenosyl-L-methionine binding By similarity
	Region	209 – 210	2	S-adenosyl-L-methionine binding By similarity
	Region	330 – 332	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	232	1	By similarity
	Binding site	99	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	229	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4WQB9 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 412C1E1D6D189D40	FASTA	358	39,946
	10 20 30 40 50 60 MTPPPPKRQK RDEYRKATAE ATSQSGASDV AEIKLPKKKY YRQRAHANPF SDHHLKYPLS 70 80 90 100 110 120 PAHMDWSSHY PAFVNPDPSH INLAGARRLL KDVEVVDIGC GFGGLLIGLA PLLPESLIVG 130 140 150 160 170 180 MEIRVSVLEY VTTRIQALRA QQQKLRAATA TATAASETPS QQQAQIDGKQ ANANAAADAA 190 200 210 220 230 240 SPAPSTDTEH MPTTLVPGSY ENISAIRSNT MKFFPNFFAR HQLSKIFICF PDPHFKARKH 250 260 270 280 290 300 KARIISETLN AEYAYALRPG GLLYTITDVE EYHHWILRHF GVELGAEEES EEKSTSPNAN 310 320 330 340 350 ANAGVRELFE RVSEEELEKD ECVRVMKEAT EEGKKVARNK GNKYVAVFRR KTDPEWPA

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000005 Genomic DNA. Translation: EAL89565.1.
RefSeq	XP_751603.1. XM_746510.1.
3D structure databases
ProteinModelPortal	Q4WQB9.
ModBase	Search...
Protein-protein interaction databases
STRING	5085.CADAFUAP00008111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00008111; CADAFUAP00008111; CADAFUAG00008111.
GeneID	3509169.
KEGG	afm:AFUA_4G12280.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OMA	LPIRTRP.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_ASPFU

Accession

Primary (citable) accession number: Q4WQB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program