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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei229S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei232UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:AFUA_4G12280
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu4g12280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705861 – 358tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST358

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi5085.CADAFUBP00006745

Structurei

3D structure databases

ProteinModelPortaliQ4WQB9
SMRiQ4WQB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 123S-adenosyl-L-methionine bindingUniRule annotation2
Regioni209 – 210S-adenosyl-L-methionine bindingUniRule annotation2
Regioni330 – 332S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965
InParanoidiQ4WQB9
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q4WQB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPPPPKRQK RDEYRKATAE ATSQSGASDV AEIKLPKKKY YRQRAHANPF
60 70 80 90 100
SDHHLKYPLS PAHMDWSSHY PAFVNPDPSH INLAGARRLL KDVEVVDIGC
110 120 130 140 150
GFGGLLIGLA PLLPESLIVG MEIRVSVLEY VTTRIQALRA QQQKLRAATA
160 170 180 190 200
TATAASETPS QQQAQIDGKQ ANANAAADAA SPAPSTDTEH MPTTLVPGSY
210 220 230 240 250
ENISAIRSNT MKFFPNFFAR HQLSKIFICF PDPHFKARKH KARIISETLN
260 270 280 290 300
AEYAYALRPG GLLYTITDVE EYHHWILRHF GVELGAEEES EEKSTSPNAN
310 320 330 340 350
ANAGVRELFE RVSEEELEKD ECVRVMKEAT EEGKKVARNK GNKYVAVFRR

KTDPEWPA
Length:358
Mass (Da):39,946
Last modified:July 5, 2005 - v1
Checksum:i412C1E1D6D189D40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000005 Genomic DNA Translation: EAL89565.1
RefSeqiXP_751603.1, XM_746510.1

Genome annotation databases

EnsemblFungiiCADAFUAT00008111; CADAFUAP00008111; CADAFUAG00008111
GeneIDi3509169
KEGGiafm:AFUA_4G12280

Similar proteinsi

Entry informationi

Entry nameiTRMB_ASPFU
AccessioniPrimary (citable) accession number: Q4WQB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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