Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Psi-producing oxygenase A

Gene

ppoA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional heme-containing enzyme that oxidizes linoleic acid to (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate (within the N-terminal heme peroxidase domain), which is subsequently isomerized to (5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate (within the C-terminal P450 heme thiolate domain). Oxidized unsaturated fatty acids, so-called oxylipins, derived from endogenous fatty acids, influence the development of the asexual conidiophores and sexual cleistothecia and regulate the secondary metabolism. These substances were collectively named psi factors and are primarily a mixture of hydroxylated oleic, linoleic and alpha-linolenic acids. They are termed psi-beta, psi-alpha, and psi-gamma, respectively. Oxylipins may also serve as activators of mammalian immune responses contributing to enhanced resistance to opportunistic fungi and as factors that modulate fungal development contributing to resistance to host defenses.1 Publication

Catalytic activityi

Linoleate + O2 = (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate.
(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate = (5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi202 – 2021Iron (heme axial ligand)PROSITE-ProRule annotation
Active sitei374 – 3741Sequence analysis
Metal bindingi377 – 3771Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • oxylipin biosynthetic process Source: ASPGD
  • pathogenesis Source: UniProtKB-KW
  • prostaglandin biosynthetic process Source: ASPGD
  • response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Isomerase, Oxidoreductase, Peroxidase

Keywords - Biological processi

Virulence

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei5289. AfumLDS01.

Names & Taxonomyi

Protein namesi
Recommended name:
Psi-producing oxygenase A
Alternative name(s):
Fatty acid oxygenase ppoA
Including the following 2 domains:
Linoleate 8R-lipoxygenase (EC:1.13.11.60)
9,12-octadecadienoate 8-hydroperoxide 8R-isomerase (EC:5.4.4.5)
Gene namesi
Name:ppoA
ORF Names:AFUA_4G10770
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g10770.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10791079Psi-producing oxygenase APRO_0000416227Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ4WPX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 446342Linoleate 8R-lipoxygenaseBy similarityAdd
BLAST
Regioni654 – 10794269,12-octadecadienoate 8-hydroperoxide 8R-isomeraseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peroxidase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000190920.
InParanoidiQ4WPX2.
KOiK17863.
OMAiTMLKVIG.
OrthoDBiEOG092C0D86.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
1.10.640.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
[Graphical view]
PfamiPF03098. An_peroxidase. 2 hits.
PF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF48264. SSF48264. 2 hits.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WPX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKQTGSAN GGLGKTLAQL EQVVSASLRP LPSQTGDGTY VTEQVKTGIL
60 70 80 90 100
KDLSHVDLGD LKTLVDVSKS ALTGEALDDR KYIMERVIQL SAGLPSTSQI
110 120 130 140 150
GKELTNTFLT TLWNDLEHPP ISYLGRDAMY RRADGSGNNV LWPHIGAAGT
160 170 180 190 200
PYARSVQPKT VQSPNLPDPE TLFDCLLARK EYKEHPNKIS SVLFYIASII
210 220 230 240 250
IHDLFETDRK DPAISLTSSY LDLSPLYGNN QQEQDLIRTF KDGKLKPDCF
260 270 280 290 300
STKRVLGFPP DVGVVLIMFN RFHNYVVEKL AMINEGGRFT KPQESDTAAY
310 320 330 340 350
AKYDNDLFQT GRLVTCGLYV NIILKDYVRT ILNINRTDSI WSLDPRSEMK
360 370 380 390 400
DGLLGRAAAQ ATGNQVAAEF NLVYRWHSCI SQRDQKWTED MYQELFPGQD
410 420 430 440 450
PSKISLQDFL RGLGRWEAKL PGEPRERPFA GLQRKADGSY DDNDLVKIFE
460 470 480 490 500
ESVEDCAGAF GALHVPTVFR SIEALGIQQA RSWNLATLNE FRKYFNLAPY
510 520 530 540 550
KTFEEINSDP YVADQLKRLY DHPDRVEIYP GIIVEDAKES MAPGSGLCTN
560 570 580 590 600
FTISRAILSD AVALVRGDRF HTVDFTPKHL TNWAYNEIQP QDSVDQTHVF
610 620 630 640 650
YKLVLRAFPN HFRGDSIYAH FPLVVPSENK KILTKLGTAD KYSWDRPNYT
660 670 680 690 700
PPPQFINSHS ACMSILSDQE TFKVTWGSKI EFLMRHNNQP YGRDFMLSGD
710 720 730 740 750
RTPNAMSRQM MGKALYRDKW ETEVKRFYEN ITLKLLHRYS YKLAGVNQVD
760 770 780 790 800
VVRDIANLAQ VHFCASVFSL PLKTESNPRG IFTESELYQI MAVVFTSIFY
810 820 830 840 850
DADIGKSFEL NQAARAVTQQ LGQLTLANVE LIAKTGFIAN LVNSLHRHDV
860 870 880 890 900
LSEYGVHMIQ RLLDSGMPAP EIVWTHVLPT AGGMVANQAQ LFSQSLDYYL
910 920 930 940 950
SEEGSVHLPE INRLAKEDTT EADDLLLRYF MEGARIRSSV ALPRVVAQPT
960 970 980 990 1000
VVEDNGQKIT LKQGQHIICN LVSASMDPVT FPEPDKVKLD RDMNLYAHFG
1010 1020 1030 1040 1050
FGPHQCLGLG LCKTALTTML KVIGRLDNLR RAPGGQGKLK KLSGPGGIAM
1060 1070
YMTPDQTAFF PFPTTMKIQW DGDLPEVKE
Length:1,079
Mass (Da):121,250
Last modified:July 5, 2005 - v1
Checksum:iD6DFE2DD2A1CE77D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU020166 mRNA. Translation: ABV21631.1.
AAHF01000005 Genomic DNA. Translation: EAL89712.1.
RefSeqiXP_751750.1. XM_746657.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00008101; CADAFUAP00008101; CADAFUAG00008101.
GeneIDi3509289.
KEGGiafm:AFUA_4G10770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU020166 mRNA. Translation: ABV21631.1.
AAHF01000005 Genomic DNA. Translation: EAL89712.1.
RefSeqiXP_751750.1. XM_746657.1.

3D structure databases

ProteinModelPortaliQ4WPX2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

PeroxiBasei5289. AfumLDS01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00008101; CADAFUAP00008101; CADAFUAG00008101.
GeneIDi3509289.
KEGGiafm:AFUA_4G10770.

Organism-specific databases

EuPathDBiFungiDB:Afu4g10770.

Phylogenomic databases

HOGENOMiHOG000190920.
InParanoidiQ4WPX2.
KOiK17863.
OMAiTMLKVIG.
OrthoDBiEOG092C0D86.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
1.10.640.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
[Graphical view]
PfamiPF03098. An_peroxidase. 2 hits.
PF00067. p450. 1 hit.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF48264. SSF48264. 2 hits.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPOA_ASPFU
AccessioniPrimary (citable) accession number: Q4WPX2
Secondary accession number(s): A7YMT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.