Q4WNE4 (BGALC_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-galactosidase C
EC=3.2.1.23

Alternative name(s):
Lactase C

Gene names

Name:	lacC
ORF Names:	AFUA_6G06660

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 983	960	Probable beta-galactosidase C		PRO_0000395237
Sites
	Active site	188	1	Proton donor Potential
	Active site	287	1	Nucleophile Potential
	Binding site	82	1	Substrate By similarity
	Binding site	127	1	Substrate By similarity
	Binding site	128	1	Substrate; via amide nitrogen By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	353	1	Substrate By similarity
Amino acid modifications
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	391	1	N-linked (GlcNAc...) Potential
	Glycosylation	434	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	516	1	N-linked (GlcNAc...) Potential
	Glycosylation	601	1	N-linked (GlcNAc...) Potential
	Glycosylation	676	1	N-linked (GlcNAc...) Potential
	Glycosylation	714	1	N-linked (GlcNAc...) Potential
	Glycosylation	719	1	N-linked (GlcNAc...) Potential
	Glycosylation	758	1	N-linked (GlcNAc...) Potential
	Glycosylation	804	1	N-linked (GlcNAc...) Potential
	Disulfide bond	257 ↔ 304		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4WNE4 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 61D896C6685A50A5	FASTA	983	108,035
	10 20 30 40 50 60 MRIFSFLFLL LLGILTGQGL VSGTDNGKTT DVTWDKYSLS VKGQRLFVFS GEFHYQRLPV 70 80 90 100 110 120 PELWLDVFQK LRANGFNAIS VYFFWSFHSA SEGEFDFENG AHDIQRLFDY AKEAGLYVIA 130 140 150 160 170 180 RAGPYCNAET SAGGFALWAA NGQMGNERTS DEAYYEKWRP WILEVGKIIA KNQITNGGPV 190 200 210 220 230 240 ILNQHENELV ETTYDPNHTL VVYMKQIAQV FEEAGIVVPS SHNEKGMRGV SWSTDYHNVG 250 260 270 280 290 300 GAVNIYGLDS YPGGLSCTNP NSGFNLVRTY HQWFQNYSFT QPSYLPEFEG GWFQPWGGSF 310 320 330 340 350 360 YDTCATELSP EFPDVYYKNN IGSRVTLHSI YMTYGGTNWG HSAAPVVYTS YDYAAPLRET 370 380 390 400 410 420 REIRDKLKQT KLIGLFTRVS KDLLKTYMEG NGTGYTSDSS IYTWSLRNPD TNAGFYVLAH 430 440 450 460 470 480 STSSTRDVTT FTLNVTTSAG AISIPDIELN GRQSKIIVTD YNFGTNSTLL FSSAEVLTYA 490 500 510 520 530 540 NLDVNVLVFY LNVGQKGTFV FKDEPKLAFQ TYGNSNLTTS ESSYGTQYSY TQGKGVTAVK 550 560 570 580 590 600 FSNGVLAYFL DKESAWNFFA PPTTSSPQVA PNEHILVQGP YLVRGASVNH GTVEITGDNA 610 620 630 640 650 660 NTTSIEVYTG NSQVKKIKWN GKTIETRKTA YGSLIGTAPG AEDVKIQLPS LDSWKAQDTL 670 680 690 700 710 720 PEIQPDYDDS KWTVCNKTTS VNAIAPLSLP VLYSGDYGYH AGTKVYRGRF DGRNVTGANV 730 740 750 760 770 780 TVQNGAAAGW AAWVNGQYAG GSAGSPNLAA TSAVLTFNSS SLKDQDNVLT VVTDYTGHDQ 790 800 810 820 830 840 NSVRPKGTQN PRGILGATLI GGGNFTSWRI QGNAGGEKNI DPVRGPMNEG GLYGERMGWH 850 860 870 880 890 900 LPGYKVPKSA SKSSPLDGVS GAEGRFYTTT FKLKLDKDLD VPIGLQLGAP EGTKAVVQVF 910 920 930 940 950 960 MNGYQFGHYL PHTGPQSLFP FPPGVINNRG ENTLAISMWA LTDAGAKLDK VELVAYGKYR 970 980 SGFDFNQDWG YLQPGWKDRS QYA

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000006 Genomic DNA. Translation: EAL88520.1.
RefSeq	XP_750558.1. XM_745465.1.
3D structure databases
ProteinModelPortal	Q4WNE4.
ModBase	Search...
Protein-protein interaction databases
STRING	5085.CADAFUAP00002274.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00002274; CADAFUAP00002274; CADAFUAG00002274.
GeneID	3508765.
KEGG	afm:AFUA_6G06660.
Phylogenomic databases
eggNOG	COG1874.
HOGENOM	HOG000181922.
KO	K01190.
OrthoDB	EOG4T1MVN.
Family and domain databases
Gene3D	2.102.20.10. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR018954. Betagal_dom2. IPR025972. BetaGal_dom3. IPR025300. BetaGal_jelly_roll_dom. IPR008979. Galactose-bd-like. IPR013781. Glyco_hydro_catalytic_dom. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR23421. PTHR23421. 1 hit.
Pfam	PF10435. BetaGal_dom2. 1 hit. PF13363. BetaGal_dom3. 1 hit. PF13364. BetaGal_dom4_5. 2 hits. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SMART	SM01029. BetaGal_dom2. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGALC_ASPFU

Accession

Primary (citable) accession number: Q4WNE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program