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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041FMNUniRule annotation
Binding sitei296 – 2961NADPUniRule annotation
Binding sitei552 – 5521NADPUniRule annotation
Binding sitei656 – 6561NADPUniRule annotation
Binding sitei694 – 6941FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 776FMNUniRule annotation
Nucleotide bindingi123 – 1264FMNUniRule annotation
Nucleotide bindingi169 – 17810FMNUniRule annotation
Nucleotide bindingi451 – 4544FADUniRule annotation
Nucleotide bindingi469 – 4713FADUniRule annotation
Nucleotide bindingi486 – 4894FADUniRule annotation
Nucleotide bindingi614 – 6152NADPUniRule annotation
Nucleotide bindingi620 – 6245NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:cprAUniRule annotation
ORF Names:AFUA_6G10990
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiFungiDB:Afu6g10990.

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalUniRule annotation
Transmembranei9 – 3123HelicalUniRule annotationAdd
BLAST
Topological domaini32 – 695664CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_0000404329Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ4WM67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 221156Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini277 – 538262FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027.
InParanoidiQ4WM67.
KOiK00327.
OMAiYWAVPKD.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WM67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLDTLDIV VLVVLLVGSV AYFTKGSYWA VPKDPYAAAN SAMNGAAKTG
60 70 80 90 100
KTRDIIQKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEDYDYENLD KFPEDKIAFF VLATYGEGEP TDNAVEFYQF ITGEDVAFES
160 170 180 190 200
GASAEEKPLS SLKYVAFGLG NNTYEHYNAM VRHVDAALTK LGAQRIGTAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WAALSESMNL QEREAVYEPV FSVIEDESLS
260 270 280 290 300
PEDDSVYLGE PTQGHLSGSP KGPYSAHNPY IAPIVESREL FTAKDRNCLH
310 320 330 340 350
MEIGIAGSNL TYQTGDHIAI WPTNAGVEVD RFLEVFGIEK KRHTVINIKG
360 370 380 390 400
LDVTAKVPIP TPTTYDAAVR FYMEICAPVS RQFVSSLVPF APDEESKAEI
410 420 430 440 450
VRLGNDKDYF HEKISNQCFN IAQALQNITS KPFSAVPFSL LIEGLNRLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHIVKGVTTN YLLALKQKQN
510 520 530 540 550
GDPSPDPHGL TYAITGPRNK YDGIHVPVHV RHSNFKLPSD PSKPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAES GKDVGPTILF FGCRNRNEDF LYKEEWKVYQ
610 620 630 640 650
EKLGDKLKII TAFSRETAKK VYVQHRLQEH ADLVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAKSRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,783
Last modified:July 5, 2005 - v1
Checksum:iAB6C6208F70E9112
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA. Translation: EAL88947.1.
RefSeqiXP_750985.1. XM_745892.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00001977; CADAFUAP00001977; CADAFUAG00001977.
GeneIDi3508290.
KEGGiafm:AFUA_6G10990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA. Translation: EAL88947.1.
RefSeqiXP_750985.1. XM_745892.1.

3D structure databases

ProteinModelPortaliQ4WM67.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00001977; CADAFUAP00001977; CADAFUAG00001977.
GeneIDi3508290.
KEGGiafm:AFUA_6G10990.

Organism-specific databases

EuPathDBiFungiDB:Afu6g10990.

Phylogenomic databases

HOGENOMiHOG000282027.
InParanoidiQ4WM67.
KOiK00327.
OMAiYWAVPKD.
OrthoDBiEOG744TKC.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.
  2. "Increased cytochrome P-450 activity in Aspergillus fumigatus after xenobiotic exposure."
    Baillie G.S., Hitchcock C.A., Burnet F.R.
    J. Med. Vet. Mycol. 34:341-347(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNCPR_ASPFU
AccessioniPrimary (citable) accession number: Q4WM67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: July 5, 2005
Last modified: March 16, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.