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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei204FMNUniRule annotation1
Binding sitei296NADPUniRule annotation1
Binding sitei552NADPUniRule annotation1
Binding sitei656NADPUniRule annotation1
Binding sitei694FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 77FMNUniRule annotation6
Nucleotide bindingi123 – 126FMNUniRule annotation4
Nucleotide bindingi169 – 178FMNUniRule annotation10
Nucleotide bindingi451 – 454FADUniRule annotation4
Nucleotide bindingi469 – 471FADUniRule annotation3
Nucleotide bindingi486 – 489FADUniRule annotation4
Nucleotide bindingi614 – 615NADPUniRule annotation2
Nucleotide bindingi620 – 624NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:cprAUniRule annotation
ORF Names:AFUA_6G10990
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiFungiDB:Afu6g10990.

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Mitochondrion outer membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cell membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8LumenalUniRule annotation8
Transmembranei9 – 31HelicalUniRule annotationAdd BLAST23
Topological domaini32 – 695CytoplasmicUniRule annotationAdd BLAST664

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004043291 – 695NADPH--cytochrome P450 reductaseAdd BLAST695

Structurei

3D structure databases

ProteinModelPortaliQ4WM67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 221Flavodoxin-likeUniRule annotationAdd BLAST156
Domaini277 – 538FAD-binding FR-typeUniRule annotationAdd BLAST262

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027.
InParanoidiQ4WM67.
KOiK00327.
OMAiEKISNQC.
OrthoDBiEOG092C1RRH.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WM67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLDTLDIV VLVVLLVGSV AYFTKGSYWA VPKDPYAAAN SAMNGAAKTG
60 70 80 90 100
KTRDIIQKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEDYDYENLD KFPEDKIAFF VLATYGEGEP TDNAVEFYQF ITGEDVAFES
160 170 180 190 200
GASAEEKPLS SLKYVAFGLG NNTYEHYNAM VRHVDAALTK LGAQRIGTAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WAALSESMNL QEREAVYEPV FSVIEDESLS
260 270 280 290 300
PEDDSVYLGE PTQGHLSGSP KGPYSAHNPY IAPIVESREL FTAKDRNCLH
310 320 330 340 350
MEIGIAGSNL TYQTGDHIAI WPTNAGVEVD RFLEVFGIEK KRHTVINIKG
360 370 380 390 400
LDVTAKVPIP TPTTYDAAVR FYMEICAPVS RQFVSSLVPF APDEESKAEI
410 420 430 440 450
VRLGNDKDYF HEKISNQCFN IAQALQNITS KPFSAVPFSL LIEGLNRLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHIVKGVTTN YLLALKQKQN
510 520 530 540 550
GDPSPDPHGL TYAITGPRNK YDGIHVPVHV RHSNFKLPSD PSKPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAES GKDVGPTILF FGCRNRNEDF LYKEEWKVYQ
610 620 630 640 650
EKLGDKLKII TAFSRETAKK VYVQHRLQEH ADLVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAKSRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,783
Last modified:July 5, 2005 - v1
Checksum:iAB6C6208F70E9112
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA. Translation: EAL88947.1.
RefSeqiXP_750985.1. XM_745892.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00001977; CADAFUAP00001977; CADAFUAG00001977.
GeneIDi3508290.
KEGGiafm:AFUA_6G10990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA. Translation: EAL88947.1.
RefSeqiXP_750985.1. XM_745892.1.

3D structure databases

ProteinModelPortaliQ4WM67.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00001977; CADAFUAP00001977; CADAFUAG00001977.
GeneIDi3508290.
KEGGiafm:AFUA_6G10990.

Organism-specific databases

EuPathDBiFungiDB:Afu6g10990.

Phylogenomic databases

HOGENOMiHOG000282027.
InParanoidiQ4WM67.
KOiK00327.
OMAiEKISNQC.
OrthoDBiEOG092C1RRH.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_ASPFU
AccessioniPrimary (citable) accession number: Q4WM67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.