Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable 1,4-beta-D-glucan cellobiohydrolase B

Gene

cbhB

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.By similarity

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei238NucleophileBy similarity1
Active sitei243Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM1 Carbohydrate-Binding Module Family 1
GH7 Glycoside Hydrolase Family 7

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 1,4-beta-D-glucan cellobiohydrolase B (EC:3.2.1.91)
Alternative name(s):
Beta-glucancellobiohydrolase B
Exocellobiohydrolase B
Exoglucanase B
Gene namesi
Name:cbhB
ORF Names:AFUA_6G11610
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu6g11610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000039354827 – 532Probable 1,4-beta-D-glucan cellobiohydrolase BAdd BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi296N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi504 ↔ 521By similarity
Disulfide bondi515 ↔ 531By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

Expressed at high levels in the presence of carboxymethylcellulose and repressed in the presence of glucose.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5085.CADAFUBP00007567

Structurei

Secondary structure

1532
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 45Combined sources4
Beta strandi51 – 54Combined sources4
Beta strandi57 – 60Combined sources4
Helixi62 – 64Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi75 – 78Combined sources4
Turni84 – 86Combined sources3
Helixi90 – 96Combined sources7
Beta strandi97 – 99Combined sources3
Helixi104 – 108Combined sources5
Beta strandi110 – 113Combined sources4
Beta strandi116 – 130Combined sources15
Beta strandi132 – 139Combined sources8
Beta strandi151 – 158Combined sources8
Beta strandi166 – 173Combined sources8
Turni177 – 183Combined sources7
Helixi190 – 193Combined sources4
Beta strandi207 – 209Combined sources3
Beta strandi231 – 235Combined sources5
Beta strandi238 – 244Combined sources7
Beta strandi249 – 254Combined sources6
Beta strandi256 – 260Combined sources5
Beta strandi262 – 265Combined sources4
Helixi266 – 269Combined sources4
Beta strandi279 – 282Combined sources4
Turni291 – 295Combined sources5
Beta strandi299 – 301Combined sources3
Beta strandi304 – 307Combined sources4
Beta strandi312 – 320Combined sources9
Beta strandi329 – 338Combined sources10
Beta strandi341 – 344Combined sources4
Beta strandi356 – 358Combined sources3
Helixi360 – 370Combined sources11
Helixi375 – 378Combined sources4
Helixi381 – 391Combined sources11
Beta strandi393 – 401Combined sources9
Turni403 – 407Combined sources5
Helixi408 – 411Combined sources4
Beta strandi412 – 415Combined sources4
Turni423 – 425Combined sources3
Helixi437 – 443Combined sources7
Beta strandi448 – 458Combined sources11
Turni459 – 464Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V1ZX-ray1.78A28-466[»]
4V20X-ray1.50A28-466[»]
ProteinModelPortaliQ4WM08
SMRiQ4WM08
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini496 – 532CBM1PROSITE-ProRule annotationAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 461CatalyticAdd BLAST435
Regioni462 – 496Thr-rich linkerAdd BLAST35

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182210
InParanoidiQ4WM08
KOiK01225
OMAiEANSMSN
OrthoDBiEOG092C1XF1

Family and domain databases

CDDicd07999 GH7_CBH_EG, 1 hit
Gene3Di2.70.100.10, 1 hit
InterProiView protein in InterPro
IPR035971 CBD_sf
IPR000254 Cellulose-bd_dom_fun
IPR013320 ConA-like_dom_sf
IPR001722 Glyco_hydro_7
IPR037019 Glyco_hydro_7_sf
PANTHERiPTHR33753 PTHR33753, 1 hit
PfamiView protein in Pfam
PF00734 CBM_1, 1 hit
PF00840 Glyco_hydro_7, 1 hit
PRINTSiPR00734 GLHYDRLASE7
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001821 CBD_fun, 1 hit
SMARTiView protein in SMART
SM00236 fCBD, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF57180 SSF57180, 1 hit
PROSITEiView protein in PROSITE
PS00562 CBM1_1, 1 hit
PS51164 CBM1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WM08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASTFSYRM YKTALILAAL LGSGQAQQVG TSQAEVHPSM TWQSCTAGGS
60 70 80 90 100
CTTNNGKVVI DANWRWVHKV GDYTNCYTGN TWDTTICPDD ATCASNCALE
110 120 130 140 150
GANYESTYGV TASGNSLRLN FVTTSQQKNI GSRLYMMKDD STYEMFKLLN
160 170 180 190 200
QEFTFDVDVS NLPCGLNGAL YFVAMDADGG MSKYPTNKAG AKYGTGYCDS
210 220 230 240 250
QCPRDLKFIN GQANVEGWQP SSNDANAGTG NHGSCCAEMD IWEANSISTA
260 270 280 290 300
FTPHPCDTPG QVMCTGDACG GTYSSDRYGG TCDPDGCDFN SFRQGNKTFY
310 320 330 340 350
GPGMTVDTKS KFTVVTQFIT DDGTSSGTLK EIKRFYVQNG KVIPNSESTW
360 370 380 390 400
TGVSGNSITT EYCTAQKSLF QDQNVFEKHG GLEGMGAALA QGMVLVMSLW
410 420 430 440 450
DDHSANMLWL DSNYPTTASS TTPGVARGTC DISSGVPADV EANHPDAYVV
460 470 480 490 500
YSNIKVGPIG STFNSGGSNP GGGTTTTTTT QPTTTTTTAG NPGGTGVAQH
510 520 530
YGQCGGIGWT GPTTCASPYT CQKLNDYYSQ CL
Length:532
Mass (Da):56,457
Last modified:July 5, 2005 - v1
Checksum:i03EBC7D15A5EAE78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA Translation: EAL89006.1
RefSeqiXP_751044.1, XM_745951.1

Genome annotation databases

EnsemblFungiiCADAFUAT00002184; CADAFUAP00002184; CADAFUAG00002184
GeneIDi3508349
KEGGiafm:AFUA_6G11610

Similar proteinsi

Entry informationi

Entry nameiCBHB_ASPFU
AccessioniPrimary (citable) accession number: Q4WM08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: July 5, 2005
Last modified: April 25, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health