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Protein

Probable alpha-L-arabinofuranosidase B

Gene

abfB

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Alpha-L-arabinofuranosidase involved in the degradation of arabinoxylan, a major component of plant hemicellulose. Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysaccharides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi: L-arabinan degradation

This protein is involved in the pathway L-arabinan degradation, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway L-arabinan degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei184 – 185Cis-peptide bondBy similarity2
Binding sitei227SubstrateBy similarity1
Active sitei229NucleophileBy similarity1
Binding sitei230Substrate; via amide nitrogenBy similarity1
Binding sitei304Substrate; via amide nitrogenBy similarity1
Active sitei305Proton donorBy similarity1
Binding sitei424SubstrateBy similarity1
Binding sitei426Substrate; via amide nitrogenBy similarity1
Binding sitei427Substrate; via amide nitrogenBy similarity1
Binding sitei443SubstrateBy similarity1
Binding sitei471SubstrateBy similarity1
Binding sitei476Substrate; via amide nitrogenBy similarity1
Binding sitei496SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Enzyme and pathway databases

UniPathwayiUPA00667

Names & Taxonomyi

Protein namesi
Recommended name:
Probable alpha-L-arabinofuranosidase B (EC:3.2.1.55)
Short name:
ABF B
Short name:
Arabinosidase B
Gene namesi
Name:abfB
ORF Names:AFUA_6G14620
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu6g14620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000039460527 – 506Probable alpha-L-arabinofuranosidase BAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 39By similarity
Disulfide bondi89 ↔ 94By similarity
Disulfide bondi184 ↔ 185By similarity
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi409 ↔ 447By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ4WL66

Structurei

3D structure databases

ProteinModelPortaliQ4WL66
SMRiQ4WL66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 343CatalyticBy similarityAdd BLAST317
Regioni344 – 506ABDBy similarityAdd BLAST163

Domaini

Organized into two domains: an N-terminal catalytic domain and a C-terminal arabinose-binding domain (ABD).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 54 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000187007
InParanoidiQ4WL66
KOiK20844
OMAiYPTRYFR
OrthoDBiEOG092C3Z3T

Family and domain databases

InterProiView protein in InterPro
IPR015289 A-L-arabinofuranosidase_B_cat
IPR038964 ABFB
IPR007934 AbfB_ABD
IPR036195 AbfB_ABD_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR39447 PTHR39447, 1 hit
PfamiView protein in Pfam
PF05270 AbfB, 1 hit
PF09206 ArabFuran-catal, 1 hit
SUPFAMiSSF110221 SSF110221, 1 hit
SSF49899 SSF49899, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WL66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPQLSIERA SVFALGLIAT GSLVVAGPCD IYSAGGTPCV AAHSTTRALY
60 70 80 90 100
SSYSGPLYQV KRGSDGATAD IAPLSAGGVA NAAAQDSFCD GTTCLITIIY
110 120 130 140 150
DQSGRGNHLT QAPPGGFSGP ESNGYDNLAS AIGAPVTLNG QKAYGVFISP
160 170 180 190 200
GTGYRNNAAS GTATGDAPEG MYAVLDGTHY NDACCFDYGN AETSSRDTGN
210 220 230 240 250
GHMEAIYFGD NTIWGTGSGS GPWIMADLEN GLFSGSSPDN NSGDPSISYR
260 270 280 290 300
FLTAVVKGKQ NQWAIRGANA ASGSLSTFYN GARPSVSGYN PMSKEGAIIL
310 320 330 340 350
GIGGDNSNGA QGTFYEGVMT SGYPSDATEN SVQANIVAAK YATASLTSGP
360 370 380 390 400
KLTVGSSISL QATTPGYTTR YIAHSGSTVN TQVVSSSSST TLKQQASWTV
410 420 430 440 450
RTGLANSDCF SFESRDTPGS FLRHYNFVLQ LSANDGTKQF HEDATFCPQA
460 470 480 490 500
GLNGQGNSIR SWNYPTRYFR HYNNVLYAAS NGGVHTFDAT SSFNNDVSWV

ISTGFA
Length:506
Mass (Da):52,548
Last modified:July 5, 2005 - v1
Checksum:i7EE4CD1C4B1A3224
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA Translation: EAL89298.1
RefSeqiXP_751336.1, XM_746243.1

Genome annotation databases

EnsemblFungiiCADAFUAT00002338; CADAFUAP00002338; CADAFUAG00002338
GeneIDi3508653
KEGGiafm:AFUA_6G14620

Similar proteinsi

Entry informationi

Entry nameiABFB_ASPFU
AccessioniPrimary (citable) accession number: Q4WL66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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