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Protein

Methionine aminopeptidase 2-2

Gene

AFUA_2G01750

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei238SubstrateUniRule annotation1
Metal bindingi259Divalent metal cation 1UniRule annotation1
Metal bindingi270Divalent metal cation 1UniRule annotation1
Metal bindingi270Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi339Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei347SubstrateUniRule annotation1
Metal bindingi372Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi467Divalent metal cation 1UniRule annotation1
Metal bindingi467Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2-2UniRule annotation
Short name:
MetAP 2-2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:AFUA_2G01750
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiFungiDB:Afu2g01750.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004076381 – 486Methionine aminopeptidase 2-2Add BLAST486

Structurei

3D structure databases

ProteinModelPortaliQ4WII3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi93 – 108Lys-richAdd BLAST16

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226278.
InParanoidiQ4WII3.
KOiK01265.
OMAiHETAHYT.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4WII3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKSPEGHR QAPHASNCNE LKPANPDPQI SQNGSGSADL DRGVIGDDDD
60 70 80 90 100
DEDAEENGVN TETPNVGKLN QPPFPNLDQV HVTTTDGLCI TEKKKKRKKS
110 120 130 140 150
NKKKKKTKSG ALPATELKQT SPPRVLVSTL FPSEYPVGEL VPYDCTARTT
160 170 180 190 200
DEELRYNSRL WDDDFLPDYR QAAEIHRQVR QYAQKELIKP GATLLSIAEG
210 220 230 240 250
IEDGVRALSG HQGLEPGDFF KAGMGFPTGL CLNHIAAHWT PNPREKDVIL
260 270 280 290 300
DKGDVLKVDF GVHVNGRIVD SAFTVAFDDK YDNLLTAVRE ATNTGIKHAG
310 320 330 340 350
VDARMSDIGA AIQEVMESYE VEIDGKVFPV KAIRNITGHD ILRYHIHGGK
360 370 380 390 400
QIPFIKNNNQ DKMEEGEVYA IETFGSTGRG FLDDDVGVYG YGRNENMSGA
410 420 430 440 450
NLRLSSAKSL LKTIDASFGS IVFSRRYLER LGVKNYLLGM KNLIDNGIVE
460 470 480
CYSPLVDVKG SYTAQFEHTI LLHSGGKEVI SRGDDY
Length:486
Mass (Da):53,508
Last modified:July 5, 2005 - v1
Checksum:i940B4DC3289A7288
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000008 Genomic DNA. Translation: EAL87272.1.
RefSeqiXP_749310.1. XM_744217.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00003355; CADAFUAP00003355; CADAFUAG00003355.
GeneIDi3506721.
KEGGiafm:AFUA_2G01750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000008 Genomic DNA. Translation: EAL87272.1.
RefSeqiXP_749310.1. XM_744217.1.

3D structure databases

ProteinModelPortaliQ4WII3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM24.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00003355; CADAFUAP00003355; CADAFUAG00003355.
GeneIDi3506721.
KEGGiafm:AFUA_2G01750.

Organism-specific databases

EuPathDBiFungiDB:Afu2g01750.

Phylogenomic databases

HOGENOMiHOG000226278.
InParanoidiQ4WII3.
KOiK01265.
OMAiHETAHYT.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP22_ASPFU
AccessioniPrimary (citable) accession number: Q4WII3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 5, 2005
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.