Q4WG11 (XYNA_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,4-beta-xylanase xynf11a
Short name=Xylanase xynf11a
EC=3.2.1.8

Alternative name(s):
1,4-beta-D-xylan xylanohydrolase xynf11a

Gene names

Name:	xlnA
Synonyms:	xynf11a
ORF Names:	AFUA_3G00320

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Neosartorya fumigata

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Ref.2
Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0. Stable in the pH range from 4.0 to 8.0. Ref.2 Temperature dependence: Optimum temperature is 60 degrees Celsius. Around 53 percent activity is retained at 70 degrees Celsius. Stable from 30 to 60 degrees Celsius with maximum stability at 30 degrees Celsius.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endo-1,4-beta-xylanase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 228	210	Endo-1,4-beta-xylanase xynf11a		PRO_0000393161
Sites
	Active site	124	1	Nucleophile By similarity
	Active site	215	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4WG11 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 7DBED47138DA238F	FASTA	228	24,494
	10 20 30 40 50 60 MVSFSYLLLA CSAIGALAAP VEPETTSFNE TALHEFAERA GTPSSTGWNN GYYYSFWTDG 70 80 90 100 110 120 GGDVTYTNGA GGSYSVNWRN VGNFVGGKGW NPGSARTINY GGSFNPSGNG YLAVYGWTTN 130 140 150 160 170 180 PLIEYYVVES YGTYNPGSGG TFRGTVNTDG GTYNIYTAVR YNAPSIEGTK TFTQYWSVRT 190 200 210 220 SKRTGGTVTM ANHFNAWSRL GMNLGTHNYQ IVATEGYQSS GSASITVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of recombinant xylan degrading enzymes from Aspergillus fumigatus isolate SL1." Dabrowski S., Ahring B.K. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SL1.
[2]	"Cloning and expression of GH11 xylanase gene from Aspergillus fumigatus MKU1 in Pichia pastoris." Jeya M., Thiagarajan S., Lee J.K., Gunasekaran P. J. Biosci. Bioeng. 108:24-29(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: MKU1.
[3]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ156553 mRNA. Translation: ABA40419.1. EF375873 Genomic DNA. Translation: ABN48478.1. AAHF01000010 Genomic DNA. Translation: EAL86316.1.
RefSeq	XP_748354.1. XM_743261.1.
3D structure databases
ProteinModelPortal	Q4WG11.
SMR	Q4WG11. Positions 42-227.
ModBase	Search...
Protein family/group databases
CAZy	GH11. Glycoside Hydrolase Family 11.
mycoCLAP	XYN11A_ASPFU.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00000232; CADAFUAP00000232; CADAFUAG00000232.
GeneID	3505821.
KEGG	afm:AFUA_3G00320.
Phylogenomic databases
eggNOG	NOG05353.
HOGENOM	HOG000179135.
KO	K01181.
OMA	IEYYVVD.
OrthoDB	EOG4643MX.
Enzyme and pathway databases
UniPathway	UPA00114.
Family and domain databases
Gene3D	2.60.120.180. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001137. Glyco_hydro_11. IPR013319. Glyco_hydro_11/12. IPR018208. Glyco_hydro_11_AS. [Graphical view]
Pfam	PF00457. Glyco_hydro_11. 1 hit. [Graphical view]
PRINTS	PR00911. GLHYDRLASE11.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit. PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_ASPFU

Accession

Primary (citable) accession number: Q4WG11

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program