Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable beta-galactosidase E

Gene

lacE

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei136SubstrateBy similarity1
Binding sitei137Substrate; via amide nitrogenBy similarity1
Binding sitei138SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei261SubstrateBy similarity1
Active sitei299NucleophileSequence analysis1
Binding sitei365SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase E (EC:3.2.1.23)
Alternative name(s):
Lactase E
Gene namesi
Name:lacE
ORF Names:AFUA_3G00380
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiFungiDB:Afu3g00380.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039524320 – 1011Probable beta-galactosidase EAdd BLAST992

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi202N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi267 ↔ 316By similarity
Glycosylationi406N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Glycosylationi446N-linked (GlcNAc...)Sequence analysis1
Glycosylationi455N-linked (GlcNAc...)Sequence analysis1
Glycosylationi588N-linked (GlcNAc...)Sequence analysis1
Glycosylationi622N-linked (GlcNAc...)Sequence analysis1
Glycosylationi704N-linked (GlcNAc...)Sequence analysis1
Glycosylationi745N-linked (GlcNAc...)Sequence analysis1
Glycosylationi759N-linked (GlcNAc...)Sequence analysis1
Glycosylationi772N-linked (GlcNAc...)Sequence analysis1
Glycosylationi778N-linked (GlcNAc...)Sequence analysis1
Glycosylationi913N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ4WG05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
InParanoidiQ4WG05.
KOiK01190.
OMAiGIDAYPM.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WG05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLLKRLIA LAAAYSVAAA PSFSHHSSQD AANKRELLQD LVTWDQHSLF
60 70 80 90 100
VRGERLMIFS GEFHPFRLPV PGLWFDVFQK IKSLGFNAVS FYTDWGLMEG
110 120 130 140 150
NPGHVVTDGI WSLDEFFTAA REAGLYLIAR PGPYINAETS AGGIPGWVLR
160 170 180 190 200
RKGIIRSNSE DYLRATDTYM ATLGKIIAKA QITNGGPVIL VQPENEYTTW
210 220 230 240 250
PNVSESEFPT TMNQEVMAYA EKQLRDAGVV VPTVVNDNKN LGYFAPGTGL
260 270 280 290 300
GETDLYGIDA YPMRYDCGNP YVWPTYRFPR DWQHEHRNHS PTTPFAIMEF
310 320 330 340 350
QGGSGDGWGG VTEDGCAILV NNEAVRVVYK NNYGFGVRVF NIYMTYGGTN
360 370 380 390 400
WGNLGYYGGY TSYDYGAAIT EDRQIWREKY SEEKLQANFL KVSPAYLTST
410 420 430 440 450
PGNGVNGSYT GNKDITVTPL FGNGTTTNLY LVRHADFTST GSAQYNLSIS
460 470 480 490 500
TSVGNVTIPQ LGGSLSLNGR DSKFHITDYD VGGFNLIYSS AEVFTWAKGD
510 520 530 540 550
NKKRVLVLYG GAGELHEFAL PKHLPRPTVV EGSYVKIAKQ GSAWVVQWEV
560 570 580 590 600
AAQRRVLRAG KLEIHLLWRN DAYQHWVLEL PAKQPIANYS SPSKETVIVK
610 620 630 640 650
GGYLLRSAWI TDNDLHLTGD VNVTTPLEVI SAPKRFDGIV FNGQSLKSTR
660 670 680 690 700
SKIGNLAATV HYQPPAISLP DLKRLDWKYI DSLPEISTEY NDEGWTPLTN
710 720 730 740 750
TYTNNTREFT GPTCLYADDY GYHGGSLIYR GHFTANGDES WVFLNTSGGV
760 770 780 790 800
GFANSVWLNQ TFLGSWTGSG RNMTYPRNIS LPHELSPGEP YVFTVVIDHM
810 820 830 840 850
GQDEEAPGTD AIKFPRGILD YALSGHELSD LRWKMTGNLG GEQYQDLTRG
860 870 880 890 900
PLNEGAMYAE RQGYHLPSPP TSSWKSSNPI KEGLTGAGIG FYATSFSLDL
910 920 930 940 950
PEGYDIPLSF RFNNSASAAR SGTSYRCQLF VNGYQFGKYV NDLGPQTKFP
960 970 980 990 1000
VPEGILNYNG VNYVAVSLWA LESQGALIGG LDLVASTPIL SGYRKPAPAP
1010
QPGWKPRRGA Y
Length:1,011
Mass (Da):111,734
Last modified:July 5, 2005 - v1
Checksum:i6312E4B521800294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000010 Genomic DNA. Translation: EAL86322.1.
RefSeqiXP_748360.1. XM_743267.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00000139; CADAFUAP00000139; CADAFUAG00000139.
GeneIDi3505787.
KEGGiafm:AFUA_3G00380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000010 Genomic DNA. Translation: EAL86322.1.
RefSeqiXP_748360.1. XM_743267.1.

3D structure databases

ProteinModelPortaliQ4WG05.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00000139; CADAFUAP00000139; CADAFUAG00000139.
GeneIDi3505787.
KEGGiafm:AFUA_3G00380.

Organism-specific databases

EuPathDBiFungiDB:Afu3g00380.

Phylogenomic databases

HOGENOMiHOG000181922.
InParanoidiQ4WG05.
KOiK01190.
OMAiGIDAYPM.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGALE_ASPFU
AccessioniPrimary (citable) accession number: Q4WG05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 5, 2005
Last modified: October 5, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.