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Protein

Probable exopolygalacturonase X

Gene

pgaX

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specific in hydrolyzing the terminal glycosidic bond of polygalacturonic acid and oligogalacturonates.By similarity

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei245Proton donorPROSITE-ProRule annotation1
Active sitei268PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable exopolygalacturonase X (EC:3.2.1.67)
Short name:
ExoPG
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Poly(1,4-alpha-D-galacturonide)galacturonohydrolase
Gene namesi
Name:pgaX
ORF Names:AFUA_6G02980
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiFungiDB:Afu6g02980.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000039366824 – 432Probable exopolygalacturonase XAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi247 ↔ 264By similarity
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Glycosylationi354N-linked (GlcNAc...)Sequence analysis1
Glycosylationi364N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi392 ↔ 398By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati231 – 252PbH1 1Add BLAST22
Repeati254 – 274PbH1 2Add BLAST21
Repeati285 – 306PbH1 3Add BLAST22
Repeati327 – 348PbH1 4Add BLAST22
Repeati362 – 394PbH1 5Add BLAST33

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ4WCZ8.
KOiK01213.
OrthoDBiEOG092C2CYK.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4WCZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSYSFVQV VTLLLSLSPS VEGFTRSRND ACKPNHPFRP LPPSQPRTKT
60 70 80 90 100
CHVVSNGHGK DDSKNIMKAL HKCNNGGKVV FDANKVYTVG TALDMTFLKH
110 120 130 140 150
IDLEVLGKIQ FTNDTDYWQA NSFKHGFQNA TTFFQLGGED VNVYGGGTLD
160 170 180 190 200
GNGQVWYDLY AEDALILRPI LFGVIGLKGG TIGPLKLRYS PQWYQLVANS
210 220 230 240 250
SDVIFDGIDI SGYSSSKNEA KNTDGWDTYR SDNIVIQNSV INNGDDCVSF
260 270 280 290 300
KPNSTNILVQ NLHCNGSHGI SVGSLGQYKG EVDIVQNVLV YNISMYNASD
310 320 330 340 350
GARIKVWPGV SSAMSEDLQG GGGLGSVKNV TYNQMYIENV DWAIEVTQCY
360 370 380 390 400
GQKNLTLCNE YPSNLTISDI HFKNFRGTTS GKRDPNVGTI VCSSPNVCSD
410 420 430
IYAENIDVKS PKGTDNFVCT NVDKSLLDVN CA
Length:432
Mass (Da):47,311
Last modified:April 20, 2010 - v2
Checksum:i09F95B72D6015B72
GO

Sequence cautioni

The sequence EAL85740 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000012 Genomic DNA. Translation: EAL85740.1. Sequence problems.
RefSeqiXP_747778.1. XM_742685.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00000417; CADAFUAP00000417; CADAFUAG00000417.
GeneIDi3505169.
KEGGiafm:AFUA_6G02980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000012 Genomic DNA. Translation: EAL85740.1. Sequence problems.
RefSeqiXP_747778.1. XM_742685.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00000417; CADAFUAP00000417; CADAFUAG00000417.
GeneIDi3505169.
KEGGiafm:AFUA_6G02980.

Organism-specific databases

EuPathDBiFungiDB:Afu6g02980.

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ4WCZ8.
KOiK01213.
OrthoDBiEOG092C2CYK.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLRX_ASPFU
AccessioniPrimary (citable) accession number: Q4WCZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.