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Q4WCZ8 (PGLRX_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable exopolygalacturonase X
Short name=ExoPG
EC=3.2.1.67
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Poly(1,4-alpha-D-galacturonide)galacturonohydrolase
Gene names
Name:pgaX
ORF Names:AFUA_6G02980
OrganismNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) [Reference proteome]
Taxonomic identifier330879 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus ›

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specific in hydrolyzing the terminal glycosidic bond of polygalacturonic acid and oligogalacturonates By similarity.

Catalytic activity

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 5 PbH1 repeats.

Sequence caution

The sequence EAL85740.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 432409Probable exopolygalacturonase X
PRO_0000393668

Regions

Repeat231 – 25222PbH1 1
Repeat254 – 27421PbH1 2
Repeat285 – 30622PbH1 3
Repeat327 – 34822PbH1 4
Repeat362 – 39433PbH1 5

Sites

Active site2451Proton donor By similarity
Active site2681 By similarity

Amino acid modifications

Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1291N-linked (GlcNAc...) Potential
Glycosylation1991N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation2651N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3291N-linked (GlcNAc...) Potential
Glycosylation3541N-linked (GlcNAc...) Potential
Glycosylation3641N-linked (GlcNAc...) Potential
Disulfide bond247 ↔ 264 By similarity
Disulfide bond392 ↔ 398 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4WCZ8 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: 09F95B72D6015B72

FASTA43247,311
        10         20         30         40         50         60 
MKFSYSFVQV VTLLLSLSPS VEGFTRSRND ACKPNHPFRP LPPSQPRTKT CHVVSNGHGK 

        70         80         90        100        110        120 
DDSKNIMKAL HKCNNGGKVV FDANKVYTVG TALDMTFLKH IDLEVLGKIQ FTNDTDYWQA 

       130        140        150        160        170        180 
NSFKHGFQNA TTFFQLGGED VNVYGGGTLD GNGQVWYDLY AEDALILRPI LFGVIGLKGG 

       190        200        210        220        230        240 
TIGPLKLRYS PQWYQLVANS SDVIFDGIDI SGYSSSKNEA KNTDGWDTYR SDNIVIQNSV 

       250        260        270        280        290        300 
INNGDDCVSF KPNSTNILVQ NLHCNGSHGI SVGSLGQYKG EVDIVQNVLV YNISMYNASD 

       310        320        330        340        350        360 
GARIKVWPGV SSAMSEDLQG GGGLGSVKNV TYNQMYIENV DWAIEVTQCY GQKNLTLCNE 

       370        380        390        400        410        420 
YPSNLTISDI HFKNFRGTTS GKRDPNVGTI VCSSPNVCSD IYAENIDVKS PKGTDNFVCT 

       430 
NVDKSLLDVN CA

« Hide

References

[1]"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. expand/collapse author list , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAHF01000012 Genomic DNA. Translation: EAL85740.1. Sequence problems.
RefSeqXP_747778.1. XM_742685.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAFUAT00000417; CADAFUAP00000417; CADAFUAG00000417.
GeneID3505169.
KEGGafm:AFUA_6G02980.

Phylogenomic databases

eggNOGNOG297100.
HOGENOMHOG000217379.
KOK01238.
OrthoDBEOG4D5661.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
PROSITEPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGLRX_ASPFU
AccessionPrimary (citable) accession number: Q4WCZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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