Reviewed, UniProtKB/Swiss-Prot Q4W9B8 (K6PF_ASPFU)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase
    6PF-1-K

Gene names

Name:	pfkA
ORF Names:	AFUA_4G00960

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	6-phosphofructokinase		PRO_0000112034
Regions
	Nucleotide binding	33 – 37	5	ATP By similarity
	Nucleotide binding	191 – 195	5	ATP By similarity
	Nucleotide binding	208 – 224	17	ATP By similarity
Sites
	Active site	164	1	Proton acceptor By similarity
	Metal binding	222	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4W9B8-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 3F4A02B1B87C6DF0	FASTA	808	88,713
	10 20 30 40 50 60 MSSTQAPVEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSDCEAYAVF EGYEGLVHGG 70 80 90 100 110 120 HMIRQLHWED VRGWLSKGGT LIGSARSMAF RERAGRLKAA KNMVLRGIDA LVVCGGDGSL 130 140 150 160 170 180 TGADVFRSEW PGLLEELVKN GELTEEQIEP YKVLNIVGLV GSIDNDMSGT DATIGCYSSL 190 200 210 220 230 240 TRICDAVDDV FDTAFSHQRG FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPRDGWEDD 250 260 270 280 290 300 MCSIITKNRK ERGKRRTIVI VAEGAQDRSL NKISSSTVKD ILTQRLGLDT RVTVLGHTQR 310 320 330 340 350 360 GGPACAYDRW LSTLQGVEAV RAVLDMKPDS PSPVITIREN KIMRTPLVDA VQETKHVAKL 370 380 390 400 410 420 IHDKDFEAAM RLRDAEFKEY HFAYRNTATP DHPKMILPQD KRMRIAIIHV GAPAGGMNQA 430 440 450 460 470 480 TRAAVGYCLT RGHTPLAIHN GFPGLCRHHD DQPVGSVREV KWLESDAWVN EGGSDIGTNR 490 500 510 520 530 540 SLPSEDFETT AMCFEKYKFD ALFVVGGFEA FTAVSQLRQA RDKYPAFKIP MVVLPATISN 550 560 570 580 590 600 NVPGTEYSLG SDTCLNTLID FCDAIRQSAS SSRRRVFVIE TQGGKSGYIA TTAGLAVGAT 610 620 630 640 650 660 AVYIPEEGID IKMLSNDIDF LRENFARDKG ANRAGKLILR NECASSTYTT QVIADIFKEE 670 680 690 700 710 720 AKGRFESRSA VPGHFQQGGK PSPMDRIRAL RMAVKCMLHL ENYAGKSRDE IAADPMSAAV 730 740 750 760 770 780 IGIKGSQVLF SAMGGEDGLE ATETDWARRR PKTEFWLELQ NYVNVLSGRA SAGKPLWSCY 790 800 ESKHFPSCCR LYNTDLSIDI DPSALTSS

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000017 Genomic DNA. Translation: EAL84323.1.
RefSeq	XP_746361.1.
3D structure databases
SMR	Q4W9B8. Positions 14-359, 358-701, 404-761.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4W9B8.
Genome annotation databases
GeneID	3503711.
KEGG	afm:AFUA_4G00960.
Phylogenomic databases
eggNOG	fuNOG05452.
HOGENOM	HBG324640.
OMA	SKMGMEA.
OrthoDB	EOG98WDJC.
PhylomeDB	Q4W9B8.
Enzyme and pathway databases
BRENDA	2.7.1.11. 18841.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_ASPFU

Accession

Primary (citable) accession number: Q4W9B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 5, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)