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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AFUA_2G09790)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23ATP; via amide nitrogenUniRule annotation1
Metal bindingi117Magnesium; catalyticUniRule annotation1
Active sitei164Proton acceptorUniRule annotation1
Binding sitei199Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei263SubstrateUniRule annotation1
Binding sitei291Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei480Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei575Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei642Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei668Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei749Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 87ATPUniRule annotation2
Nucleotide bindingi116 – 119ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkA
ORF Names:AFUA_4G00960
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiFungiDB:Afu4g00960.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120341 – 808ATP-dependent 6-phosphofructokinaseAdd BLAST808

Proteomic databases

PRIDEiQ4W9B8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ4W9B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 389N-terminal catalytic PFK domain 1Add BLAST389
Regioni162 – 164Substrate bindingUniRule annotation3
Regioni206 – 208Substrate bindingUniRule annotation3
Regioni297 – 300Substrate bindingUniRule annotation4
Regioni390 – 403Interdomain linkerAdd BLAST14
Regioni404 – 808C-terminal regulatory PFK domain 2Add BLAST405
Regioni537 – 541Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni582 – 584Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni674 – 677Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000200154.
InParanoidiQ4W9B8.
KOiK00850.
OMAiGPACAYD.
OrthoDBiEOG092C0LOE.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4W9B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTQAPVEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSDCEAYAVF
60 70 80 90 100
EGYEGLVHGG HMIRQLHWED VRGWLSKGGT LIGSARSMAF RERAGRLKAA
110 120 130 140 150
KNMVLRGIDA LVVCGGDGSL TGADVFRSEW PGLLEELVKN GELTEEQIEP
160 170 180 190 200
YKVLNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAFSHQRG
210 220 230 240 250
FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPRDGWEDD MCSIITKNRK
260 270 280 290 300
ERGKRRTIVI VAEGAQDRSL NKISSSTVKD ILTQRLGLDT RVTVLGHTQR
310 320 330 340 350
GGPACAYDRW LSTLQGVEAV RAVLDMKPDS PSPVITIREN KIMRTPLVDA
360 370 380 390 400
VQETKHVAKL IHDKDFEAAM RLRDAEFKEY HFAYRNTATP DHPKMILPQD
410 420 430 440 450
KRMRIAIIHV GAPAGGMNQA TRAAVGYCLT RGHTPLAIHN GFPGLCRHHD
460 470 480 490 500
DQPVGSVREV KWLESDAWVN EGGSDIGTNR SLPSEDFETT AMCFEKYKFD
510 520 530 540 550
ALFVVGGFEA FTAVSQLRQA RDKYPAFKIP MVVLPATISN NVPGTEYSLG
560 570 580 590 600
SDTCLNTLID FCDAIRQSAS SSRRRVFVIE TQGGKSGYIA TTAGLAVGAT
610 620 630 640 650
AVYIPEEGID IKMLSNDIDF LRENFARDKG ANRAGKLILR NECASSTYTT
660 670 680 690 700
QVIADIFKEE AKGRFESRSA VPGHFQQGGK PSPMDRIRAL RMAVKCMLHL
710 720 730 740 750
ENYAGKSRDE IAADPMSAAV IGIKGSQVLF SAMGGEDGLE ATETDWARRR
760 770 780 790 800
PKTEFWLELQ NYVNVLSGRA SAGKPLWSCY ESKHFPSCCR LYNTDLSIDI

DPSALTSS
Length:808
Mass (Da):88,713
Last modified:July 5, 2005 - v1
Checksum:i3F4A02B1B87C6DF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000017 Genomic DNA. Translation: EAL84323.1.
RefSeqiXP_746361.1. XM_741268.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00002374; CADAFUAP00002374; CADAFUAG00002374.
GeneIDi3503711.
KEGGiafm:AFUA_4G00960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000017 Genomic DNA. Translation: EAL84323.1.
RefSeqiXP_746361.1. XM_741268.1.

3D structure databases

ProteinModelPortaliQ4W9B8.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ4W9B8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADAFUAT00002374; CADAFUAP00002374; CADAFUAG00002374.
GeneIDi3503711.
KEGGiafm:AFUA_4G00960.

Organism-specific databases

EuPathDBiFungiDB:Afu4g00960.

Phylogenomic databases

HOGENOMiHOG000200154.
InParanoidiQ4W9B8.
KOiK00850.
OMAiGPACAYD.
OrthoDBiEOG092C0LOE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_ASPFU
AccessioniPrimary (citable) accession number: Q4W9B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.