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Q4W9B8

- PFKA_ASPFU

UniProt

Q4W9B8 - PFKA_ASPFU

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, AFUA_4G00960
Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231ATP; via amide nitrogen By similarity
Metal bindingi117 – 1171Magnesium; catalytic By similarity
Active sitei164 – 1641Proton acceptor By similarity
Binding sitei199 – 1991Substrate; shared with dimeric partner By similarity
Binding sitei263 – 2631Substrate By similarity
Binding sitei291 – 2911Substrate; shared with dimeric partner By similarity
Binding sitei480 – 4801Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei642 – 6421Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei668 – 6681Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei749 – 7491Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 872ATP By similarity
Nucleotide bindingi116 – 1194ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
ORF Names:AFUA_4G00960
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000002530: Chromosome 4

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_0000112034Add
BLAST

Proteomic databases

PRIDEiQ4W9B8.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi5085.CADAFUAP00002374.

Structurei

3D structure databases

ProteinModelPortaliQ4W9B8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 389389N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni162 – 1643Substrate binding By similarity
Regioni206 – 2083Substrate binding By similarity
Regioni297 – 3004Substrate binding By similarity
Regioni390 – 40314Interdomain linkerUniRule annotationAdd
BLAST
Regioni404 – 808405C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni674 – 6774Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
KOiK00850.
OMAiMEYCDVV.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4W9B8-1 [UniParc]FASTAAdd to Basket

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MSSTQAPVEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSDCEAYAVF    50
EGYEGLVHGG HMIRQLHWED VRGWLSKGGT LIGSARSMAF RERAGRLKAA 100
KNMVLRGIDA LVVCGGDGSL TGADVFRSEW PGLLEELVKN GELTEEQIEP 150
YKVLNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAFSHQRG 200
FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPRDGWEDD MCSIITKNRK 250
ERGKRRTIVI VAEGAQDRSL NKISSSTVKD ILTQRLGLDT RVTVLGHTQR 300
GGPACAYDRW LSTLQGVEAV RAVLDMKPDS PSPVITIREN KIMRTPLVDA 350
VQETKHVAKL IHDKDFEAAM RLRDAEFKEY HFAYRNTATP DHPKMILPQD 400
KRMRIAIIHV GAPAGGMNQA TRAAVGYCLT RGHTPLAIHN GFPGLCRHHD 450
DQPVGSVREV KWLESDAWVN EGGSDIGTNR SLPSEDFETT AMCFEKYKFD 500
ALFVVGGFEA FTAVSQLRQA RDKYPAFKIP MVVLPATISN NVPGTEYSLG 550
SDTCLNTLID FCDAIRQSAS SSRRRVFVIE TQGGKSGYIA TTAGLAVGAT 600
AVYIPEEGID IKMLSNDIDF LRENFARDKG ANRAGKLILR NECASSTYTT 650
QVIADIFKEE AKGRFESRSA VPGHFQQGGK PSPMDRIRAL RMAVKCMLHL 700
ENYAGKSRDE IAADPMSAAV IGIKGSQVLF SAMGGEDGLE ATETDWARRR 750
PKTEFWLELQ NYVNVLSGRA SAGKPLWSCY ESKHFPSCCR LYNTDLSIDI 800
DPSALTSS 808
Length:808
Mass (Da):88,713
Last modified:July 5, 2005 - v1
Checksum:i3F4A02B1B87C6DF0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHF01000017 Genomic DNA. Translation: EAL84323.1.
RefSeqiXP_746361.1. XM_741268.1.

Genome annotation databases

EnsemblFungiiCADAFUAT00002374; CADAFUAP00002374; CADAFUAG00002374.
GeneIDi3503711.
KEGGiafm:AFUA_4G00960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAHF01000017 Genomic DNA. Translation: EAL84323.1 .
RefSeqi XP_746361.1. XM_741268.1.

3D structure databases

ProteinModelPortali Q4W9B8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5085.CADAFUAP00002374.

Proteomic databases

PRIDEi Q4W9B8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAFUAT00002374 ; CADAFUAP00002374 ; CADAFUAG00002374 .
GeneIDi 3503711.
KEGGi afm:AFUA_4G00960.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
KOi K00850.
OMAi MEYCDVV.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus."
    Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.
    , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.
    Nature 438:1151-1156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Entry informationi

Entry nameiPFKA_ASPFU
AccessioniPrimary (citable) accession number: Q4W9B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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