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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AFUA_2G09790)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23ATP; via amide nitrogenUniRule annotation1
Metal bindingi117Magnesium; catalyticUniRule annotation1
Active sitei164Proton acceptorUniRule annotation1
Binding sitei199Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei263SubstrateUniRule annotation1
Binding sitei291Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei480Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei575Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei642Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei668Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei749Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 87ATPUniRule annotation2
Nucleotide bindingi116 – 119ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkA
ORF Names:AFUA_4G00960
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu4g00960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120341 – 808ATP-dependent 6-phosphofructokinaseAdd BLAST808

Proteomic databases

PRIDEiQ4W9B8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi5085.CADAFUBP00009853

Structurei

3D structure databases

ProteinModelPortaliQ4W9B8
SMRiQ4W9B8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 389N-terminal catalytic PFK domain 1Add BLAST389
Regioni162 – 164Substrate bindingUniRule annotation3
Regioni206 – 208Substrate bindingUniRule annotation3
Regioni297 – 300Substrate bindingUniRule annotation4
Regioni390 – 403Interdomain linkerAdd BLAST14
Regioni404 – 808C-terminal regulatory PFK domain 2Add BLAST405
Regioni537 – 541Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni582 – 584Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni674 – 677Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000200154
InParanoidiQ4W9B8
KOiK00850
OMAiVQEVGWH
OrthoDBiEOG092C0LOE

Family and domain databases

HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
InterProiView protein in InterPro
IPR009161 6-Pfructokinase_euk
IPR022953 ATP_PFK
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 2 hits
PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 2 hits
TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 2 hits

Sequencei

Sequence statusi: Complete.

Q4W9B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTQAPVEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSDCEAYAVF
60 70 80 90 100
EGYEGLVHGG HMIRQLHWED VRGWLSKGGT LIGSARSMAF RERAGRLKAA
110 120 130 140 150
KNMVLRGIDA LVVCGGDGSL TGADVFRSEW PGLLEELVKN GELTEEQIEP
160 170 180 190 200
YKVLNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAFSHQRG
210 220 230 240 250
FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPRDGWEDD MCSIITKNRK
260 270 280 290 300
ERGKRRTIVI VAEGAQDRSL NKISSSTVKD ILTQRLGLDT RVTVLGHTQR
310 320 330 340 350
GGPACAYDRW LSTLQGVEAV RAVLDMKPDS PSPVITIREN KIMRTPLVDA
360 370 380 390 400
VQETKHVAKL IHDKDFEAAM RLRDAEFKEY HFAYRNTATP DHPKMILPQD
410 420 430 440 450
KRMRIAIIHV GAPAGGMNQA TRAAVGYCLT RGHTPLAIHN GFPGLCRHHD
460 470 480 490 500
DQPVGSVREV KWLESDAWVN EGGSDIGTNR SLPSEDFETT AMCFEKYKFD
510 520 530 540 550
ALFVVGGFEA FTAVSQLRQA RDKYPAFKIP MVVLPATISN NVPGTEYSLG
560 570 580 590 600
SDTCLNTLID FCDAIRQSAS SSRRRVFVIE TQGGKSGYIA TTAGLAVGAT
610 620 630 640 650
AVYIPEEGID IKMLSNDIDF LRENFARDKG ANRAGKLILR NECASSTYTT
660 670 680 690 700
QVIADIFKEE AKGRFESRSA VPGHFQQGGK PSPMDRIRAL RMAVKCMLHL
710 720 730 740 750
ENYAGKSRDE IAADPMSAAV IGIKGSQVLF SAMGGEDGLE ATETDWARRR
760 770 780 790 800
PKTEFWLELQ NYVNVLSGRA SAGKPLWSCY ESKHFPSCCR LYNTDLSIDI

DPSALTSS
Length:808
Mass (Da):88,713
Last modified:July 5, 2005 - v1
Checksum:i3F4A02B1B87C6DF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000017 Genomic DNA Translation: EAL84323.1
RefSeqiXP_746361.1, XM_741268.1

Genome annotation databases

EnsemblFungiiCADAFUAT00002374; CADAFUAP00002374; CADAFUAG00002374
GeneIDi3503711
KEGGiafm:AFUA_4G00960

Similar proteinsi

Entry informationi

Entry nameiPFKA_ASPFU
AccessioniPrimary (citable) accession number: Q4W9B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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