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Protein
Submitted name:

Mannanase

Gene

amn5A

Organism
Bacillus sp. JAMB-602
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi345 – 3451CalciumCombined sources
Metal bindingi347 – 3471CalciumCombined sources
Metal bindingi371 – 3711Calcium; via carbonyl oxygenCombined sources
Metal bindingi374 – 3741Calcium; via carbonyl oxygenCombined sources
Metal bindingi485 – 4851CalciumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Protein family/group databases

CAZyiCBM59. Carbohydrate-Binding Module Family 59.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Submitted name:
MannanaseImported
Gene namesi
Name:amn5AImported
OrganismiBacillus sp. JAMB-602Imported
Taxonomic identifieri244966 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence analysisAdd
BLAST
Chaini33 – 490458Sequence analysisPRO_5004246020Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKYX-ray1.65A30-490[»]
ProteinModelPortaliQ4W8M3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4W8M3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 287243CellulaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 5 (cellulase A) family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4W8M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNGFKKIFA ITLSLLLVSS ILFVSGTSTA NANSGFYVSG TTLYDANGNP
60 70 80 90 100
FVMRGINHGH AWYKDQATTA IEGIANTGAN TVRIVLSDGG QWTKDDIQTV
110 120 130 140 150
RNLISLAEDN NLVAVLEVHD ATGYDSIASL NRAVDYWIEM RSALIGKEDT
160 170 180 190 200
VIINIANEWF GSWDGAAWAD GYKQAIPRLR NAGLNNTLMI DAAGWGQFPQ
210 220 230 240 250
SIHDYGREVF NADPQRNTMF SIHMYEYAGG NASQVRTNID RVLNQDLALV
260 270 280 290 300
IGEFGHRHTN GDVDESTIMS YSEQRGVGWL AWSWKGNGPE WEYLDLSNDW
310 320 330 340 350
AGNNLTAWGN TIVNGPYGLR ETSKLSTVFT GGGSDGRTSP TTLYDFEEST
360 370 380 390 400
QGWTGSSLSR GPWTVTEWSS KGNHSLKADI QMSSNSQHYL HVIQNRSLQQ
410 420 430 440 450
NSRIQATVKH ANWGSVGNGM TARLYVKTGH GYTWYSGSFV PINGSSGTTL
460 470 480 490
SLDLSNVQNL SQVREIGVQF QSESNSSGQT SIYIDNVIVE
Length:490
Mass (Da):53,764
Last modified:July 5, 2005 - v1
Checksum:i37902A787629CE99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB119999 Genomic DNA. Translation: BAD99527.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB119999 Genomic DNA. Translation: BAD99527.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WKYX-ray1.65A30-490[»]
ProteinModelPortaliQ4W8M3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM59. Carbohydrate-Binding Module Family 59.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ4W8M3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate."
    Akita M., Takeda N., Hirasawa K., Sakai H., Kawamoto M., Yamamoto M., Grant W.D., Hatada Y., Ito S., Horikoshi K.
    Acta Crystallogr. D 60:1490-1492(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 30-490 IN COMPLEX WITH CALCIUM.
  2. "Alkaline Mannanase from a Novel Species of Alkaliphilic Bacillus."
    Takeda N., Hirasawa K., Uchimura K., Nogi Y., Hatada Y., Akita M., Usami R., Yoshida Y., Grant W.D., Ito S., Horikoshi K.
    J. Appl. Glycosci. 51:229-236(2004)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: JAMB-602Imported.

Entry informationi

Entry nameiQ4W8M3_9BACI
AccessioniPrimary (citable) accession number: Q4W8M3
Entry historyi
Integrated into UniProtKB/TrEMBL: July 5, 2005
Last sequence update: July 5, 2005
Last modified: March 16, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.