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Q4W8D0

- GLN13_ORYSJ

UniProt

Q4W8D0 - GLN13_ORYSJ

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Protein

Glutamine synthetase cytosolic isozyme 1-3

Gene

GLN1-3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_243724. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_250903. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-3 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;3
Short name:
OsGLN1;3
OsGS1;3
Gene namesi
Name:GLN1-3
Ordered Locus Names:Os03g0712800, LOC_Os03g50490
ORF Names:OSJNBb0033N16.19
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ4W8D0.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Glutamine synthetase cytosolic isozyme 1-3PRO_0000239822Add
BLAST

Proteomic databases

PaxDbiQ4W8D0.
PRIDEiQ4W8D0.

Expressioni

Tissue specificityi

Expressed at low levels in spikelets (rice flower).1 Publication

Gene expression databases

ExpressionAtlasiQ4W8D0. baseline.

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ4W8D0.
SMRiQ4W8D0. Positions 6-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ4W8D0.
KOiK01915.
OMAiHIAAYGD.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4W8D0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSLLTDLV NLDLSESTDK VIAEYIWVGG TGMDVRSKAR TLSGPVDDPS
60 70 80 90 100
KLPKWNFDGS STGQATGDDS EVILHPQAIF RDPFRKGKNI LVMCDCYAPN
110 120 130 140 150
GEPIPTNNRY NAARIFSHPD VKAEEPWYGI EQEYTLLQKH INWPLGWPLG
160 170 180 190 200
GYPGPQGPYY CAAGADKSYG RDIVDAHYKA CLFAGINISG INAEVMPGQW
210 220 230 240 250
EFQIGPVVGV SAGDHVWVAR YILERITEIA GVVVSFDPKP IPGDWNGAGA
260 270 280 290 300
HTNYSTKSMR SNGGYEVIKK AIKKLGMRHR EHIAAYGDGN ERRLTGRHET
310 320 330 340 350
ADINNFVWGV ANRGASVRVG RDTEKDGKGY FEDRRPASNM DPYLVTAMIA
360 370
ETTILWEPSH GHGHGQSNGK
Length:370
Mass (Da):40,678
Last modified:July 5, 2005 - v1
Checksum:i5A0B525D2F78CD23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB180689 mRNA. Translation: BAD99301.1.
AC082645 Genomic DNA. Translation: AAK18848.1.
RefSeqiNP_001051067.1. NM_001057602.1.
UniGeneiOs.48875.

Genome annotation databases

EnsemblPlantsiOS03T0712800-02; OS03T0712800-02; OS03G0712800.
GeneIDi4333896.
KEGGiosa:4333896.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB180689 mRNA. Translation: BAD99301.1 .
AC082645 Genomic DNA. Translation: AAK18848.1 .
RefSeqi NP_001051067.1. NM_001057602.1.
UniGenei Os.48875.

3D structure databases

ProteinModelPortali Q4W8D0.
SMRi Q4W8D0. Positions 6-357.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q4W8D0.
PRIDEi Q4W8D0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS03T0712800-02 ; OS03T0712800-02 ; OS03G0712800 .
GeneIDi 4333896.
KEGGi osa:4333896.

Organism-specific databases

Gramenei Q4W8D0.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q4W8D0.
KOi K01915.
OMAi HIAAYGD.

Enzyme and pathway databases

Reactomei REACT_243724. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_250903. Amino acid synthesis and interconversion (transamination).

Gene expression databases

ExpressionAtlasi Q4W8D0. baseline.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
    Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
    Plant J. 42:641-651(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Sasanishiki.
    Tissue: Flower.
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiGLN13_ORYSJ
AccessioniPrimary (citable) accession number: Q4W8D0
Secondary accession number(s): Q9AUQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2005
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3