Reviewed, UniProtKB/Swiss-Prot Q4W6D3 (HOG1_COCHE)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:

HOG1

Organism

Cochliobolus heterostrophus (Drechslera maydis)

Taxonomic identifier

5016 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporaceae › Cochliobolus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme By similarity. Phosphorylation is induced by osmotic stress and the presence of the antifungal agent iprodione. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Mitogen-activated protein kinase HOG1		PRO_0000289681
Regions
	Domain	20 – 299	280	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Motif	171 – 173	3	TXY
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	171	1	Phosphothreonine By similarity
	Modified residue	173	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4W6D3-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: D9A27C296D487B80	FASTA	355	40,768
	10 20 30 40 50 60 MAEFVRAQIF GTTFEITSRY TDLQPVGMGA FGLVCSAKDQ LTNQAVAVKK IMKPFSTPVL 70 80 90 100 110 120 SKRTYRELKL LKHLRHENII SLSDIFISPL EDIYFVTELL GTDLHRLLTS RPLEKQFIQY 130 140 150 160 170 180 FLYQILRGLK YVHSAGVVHR DLKPSNILVN ENCDLKICDF GLARIQDPQM TGYVSTRYYR 190 200 210 220 230 240 APEIMLTWQK YDVEVDIWSA GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVIQT 250 260 270 280 290 300 ICSENTLRFV QSLPKRERQP LANKFKNAEP DAVDLLENML VFDPRKRVRA EQALAHPYLA 310 320 330 340 350 PYHDPTDEPV ADEKFDWSFN DADLPVDTWK IMMYSEILDY HNVDAAVPEQ ENNGS

References

[1]

"Group III histidine kinase is a positive regulator of Hog1-type mitogen-activated protein kinase in filamentous fungi." Yoshimi A., Kojima K., Takano Y., Tanaka C. Eukaryot. Cell 4:1820-1828(2005) [PubMed: 16278449] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PHOSPHORYLATION. Strain: HITO7711.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB208438 Genomic DNA. Translation: BAD99295.1.
3D structure databases
HSSP	HSSP built from PDB template 2BAJ based on UniProtKB Q16539.
SMR	Q4W6D3. Positions 3-342.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 2898.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_COCHE

Accession

Primary (citable) accession number: Q4W6D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)