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Protein

DNA (cytosine-5)-methyltransferase 3A

Gene

DNMT3A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for genome-wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei629S-adenosyl-L-methionineBy similarity1
Active sitei675PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri458 – 488GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri499 – 555PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

  • core promoter sequence-specific DNA binding Source: AgBase
  • DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB
  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • DNA methylation Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to fibroblast growth factor Source: AgBase
  • response to heat Source: AgBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Repressor, Transferase
Biological processTranscription, Transcription regulation
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Protein family/group databases

REBASEi11753 M.GgaDnmt3AP

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 3A (EC:2.1.1.37)
Short name:
Dnmt3a
Gene namesi
Name:DNMT3A
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130311 – 877DNA (cytosine-5)-methyltransferase 3AAdd BLAST877

Proteomic databases

PaxDbiQ4W5Z4
PRIDEiQ4W5Z4

PTM databases

iPTMnetiQ4W5Z4

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006352

Structurei

3D structure databases

ProteinModelPortaliQ4W5Z4
SMRiQ4W5Z4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 284PWWPPROSITE-ProRule annotationAdd BLAST59
Domaini447 – 579ADDPROSITE-ProRule annotationAdd BLAST133
Domaini599 – 877SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST279

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni606 – 610S-adenosyl-L-methionine bindingBy similarity5
Regioni651 – 653S-adenosyl-L-methionine bindingBy similarity3
Regioni856 – 858S-adenosyl-L-methionine bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi448 – 496Cys-richAdd BLAST49

Domaini

The PWWP domain is essential for targeting to pericentric heterochromatin.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri458 – 488GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri499 – 555PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGHW Eukaryota
ENOG410XQ4Y LUCA
HOVERGENiHBG051381
InParanoidiQ4W5Z4
KOiK17398
PhylomeDBiQ4W5Z4

Family and domain databases

InterProiView protein in InterPro
IPR025766 ADD
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR030487 DNMT3A
IPR000313 PWWP_dom
IPR029063 SAM-dependent_MTases
PANTHERiPTHR23068:SF10 PTHR23068:SF10, 1 hit
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PF00855 PWWP, 1 hit
SMARTiView protein in SMART
SM00293 PWWP, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51533 ADD, 1 hit
PS00094 C5_MTASE_1, 1 hit
PS50812 PWWP, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

Q4W5Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVESSDTPKD TAAVPKCPPP CPEASPAEPL PNGDLEADGA QWKGTEEGGA
60 70 80 90 100
SPKSGRPEED ETESLADGET GRALENGRCT PKEGLDAPAD EGELAPSDPQ
110 120 130 140 150
KKRGRRKLLE ATEKSKEEKE ENNFDSLKME GSRGRLRGGL GWESSLRQRP
160 170 180 190 200
MQRHTFQAGD PYYISKRKRD EWLARWKREA EKKAKVIAVM NVVEETPRAE
210 220 230 240 250
PQKEEEASPP ASQQPTDPAS PNVATTPEPV VADAVDKNTS KSADDEPEYE
260 270 280 290 300
DGRGLGIGEL VWGKLRGFSW WPGRIVSWWM TGRSRAAEGT RWVMWFGDGK
310 320 330 340 350
FSVVCVEKLL PLSSFSSAFH QATYNKQPMY RKAIYEVLQV ASSRAGKIFP
360 370 380 390 400
ACPENDETDT SKVVEIQNKQ MIEWALGGFQ PSGPKGLEPP EEERNPYKEV
410 420 430 440 450
YTEMWVEPEA AAYAPPPPAK KPRKSTTEKP KVKEIIDERT RERLVYEVRQ
460 470 480 490 500
KCRNIEDICI SCGSLNVTLE HPLFIGGMCQ NCKNCFLECA YQYDDDGYQS
510 520 530 540 550
YCTICCGGRE VLMCGNNNCC RCFCVECVDL LVGPGAAQAA IKEDPWNCYM
560 570 580 590 600
CGHKGVYGLL RRREDWPSRL QMFFANNHDQ EFDPPKVYPP VPAEKRKPIR
610 620 630 640 650
VLSLFDGIAT GLLVLKDLGI QVDRYIASEV CEDSITVGMV RHQGKIMYVG
660 670 680 690 700
DVRNVTQKHI QEWGPFDLVI GGSPCNDLSI VNPARKGLYE GTGRLFFEFY
710 720 730 740 750
RLLHEARPKE GDDRPFFWLF ENVVAMGVSD KRDISRFLES NPVMIDAKEV
760 770 780 790 800
SAAHRARYFW GNLPGMNRPL ASTVNDKLEL QECLEHGRIA KFSKVRTITT
810 820 830 840 850
RSNSIKQGKD QHFPVFMNEK EDILWCTEME RVFGFPVHYT DVSNMSRLAR
860 870
QRLLGRSWSV PVIRHLFAPL KEYFACV
Length:877
Mass (Da):98,944
Last modified:July 5, 2005 - v1
Checksum:iFC7131DA1D40F84F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB214886 mRNA Translation: BAD99023.1
RefSeqiNP_001020003.1, NM_001024832.1
UniGeneiGga.6002

Genome annotation databases

GeneIDi421991
KEGGigga:421991

Similar proteinsi

Entry informationi

Entry nameiDNM3A_CHICK
AccessioniPrimary (citable) accession number: Q4W5Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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