Reviewed, UniProtKB/Swiss-Prot Q4W5Z4 (DNM3A_CHICK)

Names and origin

Protein names

Recommended name:
    DNA (cytosine-5)-methyltransferase 3A
      Short name=Dnmt3a
    EC=2.1.1.37

Gene names

Name:

DNMT3A

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	877 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the C5-methyltransferase family. Contains 1 ADD-type zinc finger. Contains 1 PWWP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 877	877	DNA (cytosine-5)-methyltransferase 3A		PRO_0000313031
Regions
	Domain	226 – 284	59	PWWP
	Zinc finger	459 – 551	93	ADD-type By similarity
	Compositional bias	448 – 496	49	Cys-rich
Sites
	Active site	675	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4W5Z4-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: FC7131DA1D40F84F	FASTA	877	98,944
	10 20 30 40 50 60 MVESSDTPKD TAAVPKCPPP CPEASPAEPL PNGDLEADGA QWKGTEEGGA SPKSGRPEED 70 80 90 100 110 120 ETESLADGET GRALENGRCT PKEGLDAPAD EGELAPSDPQ KKRGRRKLLE ATEKSKEEKE 130 140 150 160 170 180 ENNFDSLKME GSRGRLRGGL GWESSLRQRP MQRHTFQAGD PYYISKRKRD EWLARWKREA 190 200 210 220 230 240 EKKAKVIAVM NVVEETPRAE PQKEEEASPP ASQQPTDPAS PNVATTPEPV VADAVDKNTS 250 260 270 280 290 300 KSADDEPEYE DGRGLGIGEL VWGKLRGFSW WPGRIVSWWM TGRSRAAEGT RWVMWFGDGK 310 320 330 340 350 360 FSVVCVEKLL PLSSFSSAFH QATYNKQPMY RKAIYEVLQV ASSRAGKIFP ACPENDETDT 370 380 390 400 410 420 SKVVEIQNKQ MIEWALGGFQ PSGPKGLEPP EEERNPYKEV YTEMWVEPEA AAYAPPPPAK 430 440 450 460 470 480 KPRKSTTEKP KVKEIIDERT RERLVYEVRQ KCRNIEDICI SCGSLNVTLE HPLFIGGMCQ 490 500 510 520 530 540 NCKNCFLECA YQYDDDGYQS YCTICCGGRE VLMCGNNNCC RCFCVECVDL LVGPGAAQAA 550 560 570 580 590 600 IKEDPWNCYM CGHKGVYGLL RRREDWPSRL QMFFANNHDQ EFDPPKVYPP VPAEKRKPIR 610 620 630 640 650 660 VLSLFDGIAT GLLVLKDLGI QVDRYIASEV CEDSITVGMV RHQGKIMYVG DVRNVTQKHI 670 680 690 700 710 720 QEWGPFDLVI GGSPCNDLSI VNPARKGLYE GTGRLFFEFY RLLHEARPKE GDDRPFFWLF 730 740 750 760 770 780 ENVVAMGVSD KRDISRFLES NPVMIDAKEV SAAHRARYFW GNLPGMNRPL ASTVNDKLEL 790 800 810 820 830 840 QECLEHGRIA KFSKVRTITT RSNSIKQGKD QHFPVFMNEK EDILWCTEME RVFGFPVHYT 850 860 870 DVSNMSRLAR QRLLGRSWSV PVIRHLFAPL KEYFACV

References

[1]	"Evolution of the vertebrate DNMT3 gene family: a possible link between existence of DNMT3L and genomic imprinting." Yokomine T., Hata K., Tsudzuki M., Sasaki H. Cytogenet. Genome Res. 113:75-80(2006) [PubMed: 16575165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB214886 mRNA. Translation: BAD99023.1.
IPI	IPI00574401.
RefSeq	NP_001020003.1.
UniGene	Gga.6002
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	11753. M.GgaDnmt3AP.
Genome annotation databases
GeneID	421991.
KEGG	gga:421991.
Phylogenomic databases
HOVERGEN	Q4W5Z4.
Enzyme and pathway databases
BRENDA	2.1.1.37. 4.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR000313. PWWP. [Graphical view]
Pfam	PF00145. DNA_methylase. 1 hit. PF00855. PWWP. 1 hit. [Graphical view]
SMART	SM00293. PWWP. 1 hit. [Graphical view]
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. False negative. PS50812. PWWP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNM3A_CHICK

Accession

Primary (citable) accession number: Q4W5Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)