Q4W382 (L_LNYV3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Lettuce necrotic yellows virus (isolate 318) (LNYV)

Taxonomic identifier

928304 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Cytorhabdovirus

Virus host

Lactuca sativa (Garden lettuce) [TaxID: 4236]
Embergeria [TaxID: 43191]
Reichardia tingitana [TaxID: 43208]
Sonchus oleraceus (Common sowthistle) [TaxID: 50207]
Sonchus hydrophilus [TaxID: 255580]

Protein attributes

Sequence length	2068 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2068	2068	RNA-directed RNA polymerase L		PRO_0000297837
Regions
	Domain	564 – 750	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q4W382 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 45AD5205F9A1B6EE	FASTA	2,068	236,583
	10 20 30 40 50 60 MDLWNEETTP KKNAYDSLPD YHLQNPLYAI TDQLSMLKRG KRLNYRLTSS YKLMKSQSTD 70 80 90 100 110 120 IKEGDPILLK EWARNWFSET ELYVVDQTLS DCENRLSLDE TDDLHFDTHL LRESVRMWRC 130 140 150 160 170 180 EFPHDDWVRM RGMQNLLIVM NAISSRRPPP PRHTCIIPGL SKITVEGGVV LVTSSLLGFQ 190 200 210 220 230 240 PEGDKETTVF AADWVRAVSD VYTERFLVLS GAILGRCLSD EHYPQVLDLE FIINWGDTVL 250 260 270 280 290 300 RRKGNKGFKL LKAYEALVLG VVQGKSESDF IDPDRFLRNT LNDIIDDDKD LGAYAVLLIQ 310 320 330 340 350 360 RLQQIDSAHH LIQLFGLHRI WGHPLVDPAK GMEKMIIIGQ KDIVRETSRP EVMGVHFKKL 370 380 390 400 410 420 MAQGYREKHG VYPNVKGNGE LETLIKNNHD WKEVGSLDND NHWRLLRFDK TFSIPESFNL 430 440 450 460 470 480 SMIVADKSVS PTLSELKENI LRKKTVMNQE LRRGVLRWIN HDSIDPREFL EQVNDKKFPD 490 500 510 520 530 540 DHKIIGLRSK EREMNPTPRM FALMSHLMRV YVVITESMLS EHILPYFPQI TMTDSQLDLT 550 560 570 580 590 600 KKTYSTVKNQ AAKIRRSGAL YDTKTVCMSL DFEKWNGHMR KESTFHVFEA LGDLFGMENL 610 620 630 640 650 660 YNETYDIFKD SYFYLADGSY VPSMDAQGNF TPEPPYSFTG HKGGQEGLRQ KGWTIFTVVC 670 680 690 700 710 720 LDWICRKHNC TYKIMGMGDN QVLQLTMYTY RVDASGKATE RGREDMRRVL FGLFDDLLDV 730 740 750 760 770 780 FSDLGLPLKP LETWISEDLF VYGKYPVLKG VPLSMDLKKI MRIFPFSNQE TMTIENALNT 790 800 810 820 830 840 IAGNAQAATQ AAPFLGVSYL VGIFMISLCS HDMLVYHPLI AKGLREVLRE NRTWGLKFKG 850 860 870 880 890 900 AHQVKTDIKG EMVDETNLRR LMMNVPRILG GYVSFNLYGL MMRGFPDPVS LAYSQLFSWG 910 920 930 940 950 960 VPTGGSDQRD YMLRWLKPIF MPERSMRLLV EDVSSVNLLA PVTPTAGLRR VVEQYLTDGR 970 980 990 1000 1010 1020 VIKNAEFRDL MMSRDNELED VISEHLCSGE HLHIRLIHDI MESTIFGYVK SITSKVTKSS 1030 1040 1050 1060 1070 1080 TIVSLAIGKA KGDPLTRLMS DEENYFRFFM WRSVINPHYD IPACPTDLAK QVRRIGWGKE 1090 1100 1110 1120 1130 1140 LIGVTVAYPW SFLKKTDCYE SGCLCTCDDG FISLFLPDSP VTPQEWDRSI GKNPPYLGSM 1150 1160 1170 1180 1190 1200 TKEKVVISTG SKVYSGEPLV RRPINLMRVI GWFVPEESET AKIIMSCVSA VSDVNPLIFK 1210 1220 1230 1240 1250 1260 GMTEGTSGSE IHRFRDTSLK HGALCSSNYL YSTRYHVSTD TFTRYAKGAQ NYDMLFQANL 1270 1280 1290 1300 1310 1320 CAIVETTHQY VLKTNQSKEP QRKTHHYKQT CYSCINPLDE SFYDVKSSKL SQLIPSKKTN 1330 1340 1350 1360 1370 1380 KYLYVPEAKI SMTLEHVPAK SWEFGTLSSD GFDQLSVNLR LQWLTDAVAD NVVIDILNPA 1390 1400 1410 1420 1430 1440 GEESYTTTSL MDIKEHNRLF YLTIRPRDFY DQLCNRILIL AEWRCMSLSD WKTPTTEAIS 1450 1460 1470 1480 1490 1500 RAAEAIIGDT PISRWYGVTG FFSWPSSMER YYVYPEIQEP DSIPVTALSA CRSVRNSLLG 1510 1520 1530 1540 1550 1560 LLGSSRKFRG RNTRIFSEDA KASKLSLKLM VYDWVKKKKK CRACHREIGI MSAHQLSSTL 1570 1580 1590 1600 1610 1620 PNSIICPKGH YVTQGLKDLD IMRSRVTLDS LRKCCSSDEI PTEPMEKKIT EWAPLTSTTC 1630 1640 1650 1660 1670 1680 RTLFDSSSMR SELIPYSPTG IESSINVQPI PKSDLYKLIS LPTNAMYKYM EVISRNIEGI 1690 1700 1710 1720 1730 1740 MNCKTAFVTG NGLGGTSKVL SNMWPGRIIT STLLDTGDAI PQVYPNCDKG SSSYARGTVI 1750 1760 1770 1780 1790 1800 SDLMVTRVND VNHLLWGEDW KPVFQSYETD LCISDIEING ELNGESRQTM IATVTMAHDW 1810 1820 1830 1840 1850 1860 KMVIMKDYIY NMREMENRLS ILLPVFKSLE LITCNSRQRV MPEVWWIMKA RRSSGKLLGY 1870 1880 1890 1900 1910 1920 HRSVIRQIWD GVKEGINTAD WAMESVFSEI NRTIASTADM IAMTIRLKAF FSLPIVGSVL 1930 1940 1950 1960 1970 1980 PYKGSYTRLL GYLQRGKKPE DISLITSDDG KRLYLSDYEK VRSVLFGLAV GMCSSATERD 1990 2000 2010 2020 2030 2040 RMLDESEYWA IDWIPSGPHI WLPYLFKGVE RSTLIHVYDY IPMLTLIMKR ERLLFKSSSD 2050 2060 IIEFKYTNNR DSCCFPITKT AKIKFNIK

References

[1]	"Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus." Dietzgen R.G., Callaghan B., Wetzel T., Dale J.L. Virus Res. 118:16-22(2006) [PubMed: 16313992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ746199 Genomic RNA. Translation: CAG34091.1. AJ867584 Genomic RNA. Translation: CAI30426.1.
RefSeq	YP_425092.1. NC_007642.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3844362.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_LNYV3

Accession

Primary (citable) accession number: Q4W382

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 5, 2005
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program