Reviewed, UniProtKB/Swiss-Prot Q4VZL2 (NDHH_CUCSA)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase subunit H, chloroplastic
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase subunit H
    NADH-plastoquinone oxidoreductase subunit H
    NADH-plastoquinone oxidoreductase 49 kDa subunit

Gene names

Name:	ndhH
Ordered Locus Names:	CsCp111

Encoded on

Plastid; Chloroplast

Organism

Cucumis sativus (Cucumber)

Taxonomic identifier

3659 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01358
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side By similarity.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on NADH or NADPH Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	NAD(P)H-quinone oxidoreductase subunit H, chloroplastic HAMAP MF_01358		PRO_0000357982

Sequences

	Sequence		Length	Mass (Da)
	Q4VZL2-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: C4905A2A4AD1C81E	FASTA	393	45,244
	10 20 30 40 50 60 MNGPATRKDL MIVNMGPHHP SMHGVLRLIL TLDGEDVIDC EPILGYLHRG MEKIAENRTI 70 80 90 100 110 120 IQYLPYVTRW DYLATMFTEA ITVNGPEQLG NIQVPKRASY IRVIMLELSR IASHLLWLGP 130 140 150 160 170 180 FMADIGAQTP FFYIFREREL VYDLFEAATG MRMMHNFFRI GGVAADLPHG WIDKCLDFCD 190 200 210 220 230 240 YFLTAVAEYQ KLITQNPIFL ERVEGVGIIS GEEVINWGLS GPMLRASGIP WDLRKVDRYE 250 260 270 280 290 300 CYDEFDWEVQ WQKEGDSLAR YLVRLAEMTE SVKIIQQALE GIPGGPYENL EIRSFDRARS 310 320 330 340 350 360 PEWNDFDYRF ISKKPSPTFE LAKQELYVRV EAPKGELGIF LIGDQGGFPW RWKIRPPGFI 370 380 390 NLQILPQLVK RMKLADIMTI LGSIDIIMGE VDR

References

[1]	"Complete sequence and organization of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome." Kim J.-S., Jung J.D., Lee J.-A., Park H.-W., Oh K.-H., Jeong W.J., Choi D.-W., Liu J.R., Cho K.Y. Plant Cell Rep. 25:334-340(2006) [PubMed: 16362300] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Baekmibaekdadagi.
[2]	"The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: its composition and comparative analysis." Plader W.W., Yukawa Y., Sugiura M., Malepszy S. Cell. Mol. Biol. Lett. 12:584-594(2007) [PubMed: 17607527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Gy14.
[3]	"Sequencing cucumber (Cucumis sativus L.) chloroplast genomes identifies differences between chilling-tolerant and -susceptible cucumber lines." Chung S.-M., Gordon V.S., Staub J.E. Genome 50:215-225(2007) [PubMed: 17546086] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Chipper and cv. Gy14.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ119058 Genomic DNA. Translation: AAZ94704.1. DQ865975 Genomic DNA. Translation: ABI97469.1. DQ865976 Genomic DNA. Translation: ABI98800.1. AJ970307 Genomic DNA. Translation: CAJ00815.1.
RefSeq	YP_247656.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3429260.
Family and domain databases
HAMAP	MF_01358. [Tree]
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_su-49kDa_CS. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHH_CUCSA

Accession

Primary (citable) accession number: Q4VZL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)