Reviewed, UniProtKB/Swiss-Prot Q4VSN2 (DUSTY_TAKRU)

Names and origin

Protein names

Recommended name:
    Dual serine/threonine and tyrosine protein kinase
    EC=2.7.12.1
Alternative name(s):
    Dusty protein kinase
      Short name=Dusty PK
    Receptor-interacting serine/threonine-protein kinase 5

Gene names

Name:	dstyk
Synonyms:	ripk5

Organism

Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)

Taxonomic identifier

31033 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Tetraodontiformes › Tetradontoidea › Tetraodontidae › Takifugu

Protein attributes

Sequence length	921 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May induce both caspase-dependent apoptosis and caspase-independent cell death By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q4VSN2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q4VSN2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     513-540: Missing.
     817-861: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 921	921	Dual serine/threonine and tyrosine protein kinase		PRO_0000233124
Regions
	Domain	645 – 899	255	Protein kinase
	Nucleotide binding	651 – 659	9	ATP By similarity
Sites
	Active site	770	1	Proton acceptor By similarity
	Binding site	674	1	ATP By similarity
Natural variations
	Alternative sequence	513 – 540	28	Missing in isoform 2.		VSP_018041
	Alternative sequence	817 – 861	45	Missing in isoform 2.		VSP_018042

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: F3E559F57BB5485A	FASTA	921	103,845
	10 20 30 40 50 60 MEGHGPQKSS PLARDLTRAF NGYHKQTVLL KKNLKETHAF FREMRQNYSN TCASSTLSSD 70 80 90 100 110 120 SASLETSQFS CISFPSHEEE FLRNTVGAAP YILVLGQDCA ARYQLLNCLL GERLLPLGPQ 130 140 150 160 170 180 AGHACHGGQG STCKRRKLCF THGKQTRLSL ALPGQYELVH QLVANCGRWD TVPREDLEIL 190 200 210 220 230 240 DECEDPAHRQ AELEITLHHP MLQEAKVMVV PCPSVQPIEE AIEDCTRSAI PIVLYAVNQD 250 260 270 280 290 300 SLSSEQVADL WKVKEILSFP ICFVRLPNLS EDSSEPGQRF EKDKSKLQKQ LLSHGLLTCP 310 320 330 340 350 360 MGNCSCGAPT QMPTPGAKPQ SVLGENFERL HRILVPFTRQ VLQNQQVEAA SLLNGLHCRC 370 380 390 400 410 420 LDLFINQAFD MQRDLQITPR RLEYTREKEG ELFTSLMAIA NRKQEEMKDM IVETLGSMKE 430 440 450 460 470 480 QLLEDAANLE FTDIIVTTNG DPVTSKEIKS CIHQIQDLIV VRLNQAVANK LISSVDYLRE 490 500 510 520 530 540 SFVGTLERCL DSLEKSHIES SVHNITSNHL KQLLNAAYHV EVTFHSGSSV TRLFWEQIKQ 550 560 570 580 590 600 IIHRITFVNP PAITPEWKRK VAQDAIESLS AAKLARSICS QFRTRLNSSH EAFAASLRQL 610 620 630 640 650 660 EERHTGRLER TEDLWLRVRK DHAPRLARLS LESRSLRDVL LHGKPKLGRE LGRGQYGVVY 670 680 690 700 710 720 LCDNWGGHYP CALKSVVPPD DKHWNDLALE FHYTRTLPKH ERLVDLHGSV IDHTYAGGSS 730 740 750 760 770 780 IAVLLIMERL HRDLYTGLKA GLSLQERLQI ALDVVEGIRF LHNQGLLHRD IKLKNVLLDK 790 800 810 820 830 840 QNRAKITDLG FCKPEAMMSG SIVGTPIHMA PELFTGKYDN SVDVYAFGIL FWYLCAGSVK 850 860 870 880 890 900 LPEAFEKCSS KDQLWNNVKK GARPERLPCF DEECWQLMEA CWNGDPSQRP LLGIVEPSLQ 910 920 SMMVRLCCGS EQKSSSLEDS S
	Isoform 2. Checksum: CDD1B4A4EA9DB98C	FASTA	848	95,456
	10 20 30 40 50 60 MEGHGPQKSS PLARDLTRAF NGYHKQTVLL KKNLKETHAF FREMRQNYSN TCASSTLSSD 70 80 90 100 110 120 SASLETSQFS CISFPSHEEE FLRNTVGAAP YILVLGQDCA ARYQLLNCLL GERLLPLGPQ 130 140 150 160 170 180 AGHACHGGQG STCKRRKLCF THGKQTRLSL ALPGQYELVH QLVANCGRWD TVPREDLEIL 190 200 210 220 230 240 DECEDPAHRQ AELEITLHHP MLQEAKVMVV PCPSVQPIEE AIEDCTRSAI PIVLYAVNQD 250 260 270 280 290 300 SLSSEQVADL WKVKEILSFP ICFVRLPNLS EDSSEPGQRF EKDKSKLQKQ LLSHGLLTCP 310 320 330 340 350 360 MGNCSCGAPT QMPTPGAKPQ SVLGENFERL HRILVPFTRQ VLQNQQVEAA SLLNGLHCRC 370 380 390 400 410 420 LDLFINQAFD MQRDLQITPR RLEYTREKEG ELFTSLMAIA NRKQEEMKDM IVETLGSMKE 430 440 450 460 470 480 QLLEDAANLE FTDIIVTTNG DPVTSKEIKS CIHQIQDLIV VRLNQAVANK LISSVDYLRE 490 500 510 520 530 540 SFVGTLERCL DSLEKSHIES SVHNITSNHL KQIIHRITFV NPPAITPEWK RKVAQDAIES 550 560 570 580 590 600 LSAAKLARSI CSQFRTRLNS SHEAFAASLR QLEERHTGRL ERTEDLWLRV RKDHAPRLAR 610 620 630 640 650 660 LSLESRSLRD VLLHGKPKLG RELGRGQYGV VYLCDNWGGH YPCALKSVVP PDDKHWNDLA 670 680 690 700 710 720 LEFHYTRTLP KHERLVDLHG SVIDHTYAGG SSIAVLLIME RLHRDLYTGL KAGLSLQERL 730 740 750 760 770 780 QIALDVVEGI RFLHNQGLLH RDIKLKNVLL DKQNRAKITD LGFCKPEAMM SGSIVGTPIH 790 800 810 820 830 840 MAPELFTGAR PERLPCFDEE CWQLMEACWN GDPSQRPLLG IVEPSLQSMM VRLCCGSEQK SSSLEDSS

References

[1]

"Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain." Peng J., Dong W., Chen Y., Mo R., Cheng J.-F., Hui C.-C., Mohandas N., Huang C.-H. Biochim. Biophys. Acta 1759:562-572(2006) [PubMed: 17123648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY208854 mRNA. Translation: AAP42422.1. AY429680 mRNA. Translation: AAS55396.1. AY641095 mRNA. Translation: AAV40861.1.
RefSeq	NP_001027827.1.
UniGene	Tru.1977
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSTRUT00000023611; ENSTRUP00000023514; ENSTRUG00000009348; Takifugu rubripes. [Genome view] ENSTRUT00000023614; ENSTRUP00000023517; ENSTRUG00000009348; Takifugu rubripes. [Genome view] ENSTRUT00000023615; ENSTRUP00000023518; ENSTRUG00000009348; Takifugu rubripes. [Genome view]
GeneID	445996.
Organism-specific databases
CTD	445996.
Phylogenomic databases
HOVERGEN	Q4VSN2.
Enzyme and pathway databases
BRENDA	2.7.11.1. 281122.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSTY_TAKRU

Accession

Primary (citable) accession number: Q4VSN2
Secondary accession number(s): Q6XUW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2005
Last modified:	September 1, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)