Q4VNK0 (SMTA_BRAOT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenocysteine Se-methyltransferase
Short name=BoSMT
EC=2.1.1.n3

Gene names

Name:

SMT

Organism

Brassica oleracea var. italica (Broccoli)

Taxonomic identifier

36774 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica › Brassica oleracea

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the methylation of DL- and L-selenocysteine with S-methylmethionine as donor. Methylates also DL-homocysteine, DL- and L-cysteine in vitro. May be involved in selenium detoxification. Ref.1 Ref.2
Catalytic activity	S-methyl-L-methionine + L-selenocysteine = L-methionine + Se-methyl-L- selenocysteine. Ref.1 Ref.2
Cofactor	Zinc Potential.
Enzyme regulation	Inhibited by L-methionine. Ref.1
Tissue specificity	Expressed in roots, young leaves and florets, but not detected in plants not exposed to selenium. Ref.1
Induction	Up-regulated by selenate, but not by selenite. Down-regulated by sulfate. Ref.1
Sequence similarities	Contains 1 Hcy-binding domain.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Ref.1

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	response to selenium ion Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW selenocysteine methyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Selenocysteine Se-methyltransferase		PRO_0000409375
Regions
	Domain	13 – 330	318	Hcy-binding
	Compositional bias	332 – 344	13	Ser-rich
Sites
	Metal binding	248	1	Zinc Potential
	Metal binding	315	1	Zinc Potential
	Metal binding	316	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4VNK0 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 50E11284D078FC8D	FASTA	346	37,863
	10 20 30 40 50 60 MVTGNTKAET FYSMKELLKE TGGYAIIDGG LATELERHGA DLNDPLWSAK CLLTSPHLIH 70 80 90 100 110 120 TVHLDYLEAG ADIISSASYQ ATIQGFEAKG YSIEKSESLL RKSVEIACEA RSTYYDKCKD 130 140 150 160 170 180 DDDKKILKKR PILVAASVGS YGAFLADGSE YSGIYGDLIT LETLKDFHRR RVQVLAESGA 190 200 210 220 230 240 DIIAFETIPN KLEAQAFAEL LDEGVAKIPG WFSFNSKDGV NVVSGDSIKE CIAIAEACEK 250 260 270 280 290 300 VVAVGINCTP PRFIEGLVLE IAKVTSKPIL VYPNSGERYD PERKEWVENT GVGNEDFVSY 310 320 330 340 VEKWMDAGVS LLGGCCRTTP TTIRAIHKRL VSRRSLFSSS SSSSHH

References

[1]

"Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli." Lyi S.M., Heller L.I., Rutzke M., Welch R.M., Kochian L.V., Li L. Plant Physiol. 138:409-420(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION BY SELENATE AND SULFATE, ENZYME REGULATION, TISSUE SPECIFICITY. Strain: cv. Green comet.

[2]

"Biochemical and molecular characterization of the homocysteine S-methyltransferase from broccoli (Brassica oleracea var. italica)." Lyi S.M., Zhou X., Kochian L.V., Li L. Phytochemistry 68:1112-1119(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY. Strain: cv. Green comet.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY817737 mRNA. Translation: AAX20123.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15249.
Family and domain databases
Gene3D	3.20.20.330. 1 hit.
InterPro	IPR003726. S_MeTrfase. [Graphical view]
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
SUPFAM	SSF82282. S_methyl_trans. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMTA_BRAOT

Accession

Primary (citable) accession number: Q4VNK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	July 5, 2005
Last modified:	March 6, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program