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Q4VCS5

- AMOT_HUMAN

UniProt

Q4VCS5 - AMOT_HUMAN

Protein

Angiomotin

Gene

AMOT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.2 Publications

    GO - Molecular functioni

    1. angiostatin binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. receptor activity Source: MGI

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. blood vessel endothelial cell migration Source: Ensembl
    3. cell-cell junction assembly Source: UniProtKB
    4. cell migration involved in gastrulation Source: Ensembl
    5. cellular protein localization Source: MGI
    6. chemotaxis Source: Ensembl
    7. establishment of cell polarity involved in ameboidal cell migration Source: Ensembl
    8. gastrulation with mouth forming second Source: Ensembl
    9. hippo signaling Source: MGI
    10. in utero embryonic development Source: Ensembl
    11. negative regulation of angiogenesis Source: MGI
    12. negative regulation of GTPase activity Source: Ensembl
    13. negative regulation of vascular permeability Source: UniProtKB
    14. positive regulation of blood vessel endothelial cell migration Source: UniProtKB
    15. positive regulation of cell size Source: UniProtKB
    16. positive regulation of embryonic development Source: Ensembl
    17. positive regulation of stress fiber assembly Source: UniProtKB
    18. regulation of cell migration Source: MGI
    19. regulation of small GTPase mediated signal transduction Source: Ensembl
    20. vasculogenesis Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_118607. Signaling by Hippo.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angiomotin
    Gene namesi
    Name:AMOT
    Synonyms:KIAA1071
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:17810. AMOT.

    Subcellular locationi

    Cell junctiontight junction 1 Publication
    Note: Localized on the cell surface. May act as a transmembrane protein.

    GO - Cellular componenti

    1. actin filament Source: UniProtKB
    2. cell surface Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. cytosol Source: Reactome
    5. endocytic vesicle Source: MGI
    6. external side of plasma membrane Source: MGI
    7. integral component of membrane Source: UniProtKB
    8. lamellipodium Source: UniProtKB
    9. ruffle Source: MGI
    10. stress fiber Source: UniProtKB
    11. tight junction Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Tight junction

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24773.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10841084AngiomotinPRO_0000190668Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei714 – 7141PhosphoserineBy similarity

    Post-translational modificationi

    Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ4VCS5.
    PaxDbiQ4VCS5.
    PRIDEiQ4VCS5.

    PTM databases

    PhosphoSiteiQ4VCS5.

    Expressioni

    Tissue specificityi

    Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.1 Publication

    Gene expression databases

    ArrayExpressiQ4VCS5.
    BgeeiQ4VCS5.
    CleanExiHS_AMOT.
    GenevestigatoriQ4VCS5.

    Interactioni

    Subunit structurei

    Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARHGAP17Q68EM74EBI-3891843,EBI-1642807
    NF2P352408EBI-2511319,EBI-1014472

    Protein-protein interaction databases

    BioGridi127557. 62 interactions.
    IntActiQ4VCS5. 60 interactions.
    STRINGi9606.ENSP00000361027.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4VCS5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili429 – 689261Sequence AnalysisAdd
    BLAST
    Coiled coili721 – 75131Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1081 – 10844PDZ-binding

    Domaini

    The coiled coil domain interacts directly with the BAR domain of ARHGAP17.1 Publication
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.1 Publication

    Sequence similaritiesi

    Belongs to the angiomotin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG131728.
    HOGENOMiHOG000233789.
    HOVERGENiHBG066485.
    InParanoidiQ4VCS5.
    KOiK16819.
    OMAiPGKVHQD.
    OrthoDBiEOG7M6D6Q.
    PhylomeDBiQ4VCS5.
    TreeFamiTF333368.

    Family and domain databases

    InterProiIPR009114. Angiomotin.
    IPR024646. Angiomotin_C.
    [Graphical view]
    PANTHERiPTHR14826. PTHR14826. 1 hit.
    PfamiPF12240. Angiomotin_C. 1 hit.
    [Graphical view]
    PRINTSiPR01807. ANGIOMOTIN.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q4VCS5-1) [UniParc]FASTAAdd to Basket

    Also known as: p130

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS     50
    GSGNPGPQSD VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM 100
    QNNEELPTYE EAKVQSQYFR GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ 150
    RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ MSLATSGVKA HPPVTSAPLS 200
    PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP EYPFKGMPPQ 250
    SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL 300
    SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP 350
    RPQQHFLPNQ AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ 400
    QQPGEAYSAM PRAQPSSASY QPVPADPFAI VSRAQQMVEI LSDENRNLRQ 450
    ELEGCYEKVA RLQKVETEIQ RVSEAYENLV KSSSKREALE KAMRNKLEGE 500
    IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL FAKNKESQRE 550
    KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV 600
    DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT 650
    NVSEYNAAAL MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA 700
    TVAAQRDTTV ISHSPNTSYD TALEARIQKE EEEILMANKR CLDMEGRIKT 750
    LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS CMRPAKSLMS ISNAGSGLLS 800
    HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST PSPVPPSTPL 850
    LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA 900
    ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA 950
    ASVASAAAVA PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ 1000
    TQAPTSAPAV APTPAPTPTP AVAQAEVPAS PATGPGPHRL SIPSLTCNPD 1050
    KTDGPVFHSN TLERKTPIQI LGQEPDAEMV EYLI 1084
    Length:1,084
    Mass (Da):118,085
    Last modified:July 5, 2005 - v1
    Checksum:iD7E7021E9535A628
    GO
    Isoform 2 (identifier: Q4VCS5-2) [UniParc]FASTAAdd to Basket

    Also known as: p80

    The sequence of this isoform differs from the canonical sequence as follows:
         1-409: Missing.

    Show »
    Length:675
    Mass (Da):72,540
    Checksum:iEBC28B74427AD481
    GO

    Sequence cautioni

    The sequence AAH94712.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 409409Missing in isoform 2. 2 PublicationsVSP_015709Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF286598 mRNA. Translation: AAG01851.1.
    AY987378 mRNA. Translation: AAY24451.1.
    AB028994 mRNA. Translation: BAA83023.3.
    BC094712 mRNA. Translation: AAH94712.1. Sequence problems.
    CCDSiCCDS14563.1. [Q4VCS5-2]
    CCDS48154.1. [Q4VCS5-1]
    RefSeqiNP_001106962.1. NM_001113490.1. [Q4VCS5-1]
    NP_573572.1. NM_133265.2. [Q4VCS5-2]
    XP_005262144.1. XM_005262087.1. [Q4VCS5-1]
    XP_005262145.1. XM_005262088.1. [Q4VCS5-1]
    XP_005262146.1. XM_005262089.1. [Q4VCS5-1]
    XP_005262147.1. XM_005262090.1. [Q4VCS5-2]
    XP_006724686.1. XM_006724623.1. [Q4VCS5-2]
    UniGeneiHs.528051.

    Genome annotation databases

    EnsembliENST00000304758; ENSP00000305557; ENSG00000126016. [Q4VCS5-2]
    ENST00000371959; ENSP00000361027; ENSG00000126016. [Q4VCS5-1]
    ENST00000524145; ENSP00000429013; ENSG00000126016. [Q4VCS5-1]
    GeneIDi154796.
    KEGGihsa:154796.
    UCSCiuc004epr.3. human. [Q4VCS5-1]

    Polymorphism databases

    DMDMi74753814.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF286598 mRNA. Translation: AAG01851.1 .
    AY987378 mRNA. Translation: AAY24451.1 .
    AB028994 mRNA. Translation: BAA83023.3 .
    BC094712 mRNA. Translation: AAH94712.1 . Sequence problems.
    CCDSi CCDS14563.1. [Q4VCS5-2 ]
    CCDS48154.1. [Q4VCS5-1 ]
    RefSeqi NP_001106962.1. NM_001113490.1. [Q4VCS5-1 ]
    NP_573572.1. NM_133265.2. [Q4VCS5-2 ]
    XP_005262144.1. XM_005262087.1. [Q4VCS5-1 ]
    XP_005262145.1. XM_005262088.1. [Q4VCS5-1 ]
    XP_005262146.1. XM_005262089.1. [Q4VCS5-1 ]
    XP_005262147.1. XM_005262090.1. [Q4VCS5-2 ]
    XP_006724686.1. XM_006724623.1. [Q4VCS5-2 ]
    UniGenei Hs.528051.

    3D structure databases

    ProteinModelPortali Q4VCS5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127557. 62 interactions.
    IntActi Q4VCS5. 60 interactions.
    STRINGi 9606.ENSP00000361027.

    PTM databases

    PhosphoSitei Q4VCS5.

    Polymorphism databases

    DMDMi 74753814.

    Proteomic databases

    MaxQBi Q4VCS5.
    PaxDbi Q4VCS5.
    PRIDEi Q4VCS5.

    Protocols and materials databases

    DNASUi 154796.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000304758 ; ENSP00000305557 ; ENSG00000126016 . [Q4VCS5-2 ]
    ENST00000371959 ; ENSP00000361027 ; ENSG00000126016 . [Q4VCS5-1 ]
    ENST00000524145 ; ENSP00000429013 ; ENSG00000126016 . [Q4VCS5-1 ]
    GeneIDi 154796.
    KEGGi hsa:154796.
    UCSCi uc004epr.3. human. [Q4VCS5-1 ]

    Organism-specific databases

    CTDi 154796.
    GeneCardsi GC0XM112017.
    HGNCi HGNC:17810. AMOT.
    MIMi 300410. gene.
    neXtProti NX_Q4VCS5.
    PharmGKBi PA24773.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG131728.
    HOGENOMi HOG000233789.
    HOVERGENi HBG066485.
    InParanoidi Q4VCS5.
    KOi K16819.
    OMAi PGKVHQD.
    OrthoDBi EOG7M6D6Q.
    PhylomeDBi Q4VCS5.
    TreeFami TF333368.

    Enzyme and pathway databases

    Reactomei REACT_118607. Signaling by Hippo.

    Miscellaneous databases

    ChiTaRSi AMOT. human.
    GeneWikii AMOT.
    GenomeRNAii 154796.
    NextBioi 87314.
    PROi Q4VCS5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q4VCS5.
    Bgeei Q4VCS5.
    CleanExi HS_AMOT.
    Genevestigatori Q4VCS5.

    Family and domain databases

    InterProi IPR009114. Angiomotin.
    IPR024646. Angiomotin_C.
    [Graphical view ]
    PANTHERi PTHR14826. PTHR14826. 1 hit.
    Pfami PF12240. Angiomotin_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01807. ANGIOMOTIN.
    ProtoNeti Search...

    Publicationsi

    1. "Angiomotin. An angiostatin binding protein that regulates endothelial cell migration and tube formation."
      Troyanovsky B., Levchenko T., Maensson G., Matvijenko O., Holmgren L.
      J. Cell Biol. 152:1247-1254(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION.
      Tissue: Placenta.
    2. "Angiomotin regulates endothelial cell-cell junctions and cell motility."
      Bratt A., Birot O., Sinha I., Veitonmaeki N., Aase K., Ernkvist M., Holmgren L.
      J. Biol. Chem. 280:34859-34869(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TOPOLOGY, INTERACTION WITH ANGIOSTATIN AND MAGI1, SUBCELLULAR LOCATION.
    3. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543 (ISOFORM 1).
      Tissue: Spinal cord.
    6. "A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells."
      Wells C.D., Fawcett J.P., Traweger A., Yamanaka Y., Goudreault M., Elder K., Kulkarni S., Gish G., Virag C., Lim C., Colwill K., Starostine A., Metalnikov P., Pawson T.
      Cell 125:535-548(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, FUNCTION, IDENTIFICATION IN A COMPLEX WITH ARHGAP17; MPP5; INADL AND PARD3.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "The Nedd4-like ubiquitin E3 ligases target angiomotin/p130 to ubiquitin-dependent degradation."
      Wang C., An J., Zhang P., Xu C., Gao K., Wu D., Wang D., Yu H., Liu J.O., Yu L.
      Biochem. J. 444:279-289(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION BY NEDD4; NEDD4L AND ITCH.

    Entry informationi

    Entry nameiAMOT_HUMAN
    AccessioniPrimary (citable) accession number: Q4VCS5
    Secondary accession number(s): Q504X5, Q9HD27, Q9UPT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    'Motus' means 'motility' in Latin.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3