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Protein

Angiomotin

Gene

AMOT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.2 Publications

GO - Molecular functioni

  • angiostatin binding Source: UniProtKB
  • receptor activity Source: MGI

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • blood vessel endothelial cell migration Source: Ensembl
  • cell-cell junction assembly Source: UniProtKB
  • cell migration involved in gastrulation Source: Ensembl
  • cellular protein localization Source: MGI
  • chemotaxis Source: Ensembl
  • establishment of cell polarity involved in ameboidal cell migration Source: Ensembl
  • gastrulation with mouth forming second Source: Ensembl
  • hippo signaling Source: MGI
  • in utero embryonic development Source: Ensembl
  • negative regulation of angiogenesis Source: MGI
  • negative regulation of GTPase activity Source: Ensembl
  • negative regulation of vascular permeability Source: UniProtKB
  • positive regulation of blood vessel endothelial cell migration Source: UniProtKB
  • positive regulation of cell size Source: UniProtKB
  • positive regulation of embryonic development Source: Ensembl
  • positive regulation of stress fiber assembly Source: UniProtKB
  • regulation of cell migration Source: MGI
  • regulation of small GTPase mediated signal transduction Source: Ensembl
  • vasculogenesis Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-2028269. Signaling by Hippo.
SIGNORiQ4VCS5.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiomotin
Gene namesi
Name:AMOT
Synonyms:KIAA1071
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:17810. AMOT.

Subcellular locationi

GO - Cellular componenti

  • actin filament Source: UniProtKB
  • bicellular tight junction Source: UniProtKB
  • cell surface Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • endocytic vesicle Source: MGI
  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB
  • lamellipodium Source: UniProtKB
  • ruffle Source: MGI
  • stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi154796.
OpenTargetsiENSG00000126016.
PharmGKBiPA24773.

Chemistry databases

ChEMBLiCHEMBL3392949.

Polymorphism and mutation databases

BioMutaiAMOT.
DMDMi74753814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001906681 – 1084AngiomotinAdd BLAST1084

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki594Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki595Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei714PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ4VCS5.
MaxQBiQ4VCS5.
PaxDbiQ4VCS5.
PeptideAtlasiQ4VCS5.
PRIDEiQ4VCS5.

PTM databases

iPTMnetiQ4VCS5.
PhosphoSitePlusiQ4VCS5.

Expressioni

Tissue specificityi

Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.1 Publication

Gene expression databases

BgeeiENSG00000126016.
CleanExiHS_AMOT.
ExpressionAtlasiQ4VCS5. baseline and differential.
GenevisibleiQ4VCS5. HS.

Organism-specific databases

HPAiHPA067290.

Interactioni

Subunit structurei

Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGAP17Q68EM74EBI-3891843,EBI-1642807
C1orf216Q8TAB53EBI-3891843,EBI-747505
KRT15P190123EBI-3891843,EBI-739566
KRT31Q153233EBI-3891843,EBI-948001
LMO4P619683EBI-3891843,EBI-2798728
LURAP1Q96LR23EBI-3891843,EBI-741355
MAGEA4Q1RN333EBI-3891843,EBI-10194128
MED4Q9NPJ63EBI-3891843,EBI-394607
NF2P352408EBI-2511319,EBI-1014472
Nf2P466622EBI-2511319,EBI-644586From a different organism.
PIN1Q135263EBI-3891843,EBI-714158
PPP2R4Q152573EBI-3891843,EBI-1774121
SEPT1Q8WYJ63EBI-3891843,EBI-693002
SNAPC5O759713EBI-3891843,EBI-749483
UNC119Q134323EBI-3891843,EBI-711260
WDYHV1Q96HA83EBI-3891843,EBI-741158

GO - Molecular functioni

  • angiostatin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127557. 102 interactors.
DIPiDIP-53711N.
IntActiQ4VCS5. 79 interactors.
STRINGi9606.ENSP00000361027.

Structurei

3D structure databases

ProteinModelPortaliQ4VCS5.
SMRiQ4VCS5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili429 – 689Sequence analysisAdd BLAST261
Coiled coili721 – 751Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1081 – 1084PDZ-binding4

Domaini

The coiled coil domain interacts directly with the BAR domain of ARHGAP17.1 Publication
The angiostatin binding domain (871-1005) allows the binding to angiostatin.1 Publication

Sequence similaritiesi

Belongs to the angiomotin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOGENOMiHOG000233789.
HOVERGENiHBG066485.
InParanoidiQ4VCS5.
KOiK16819.
OMAiELDGCYE.
OrthoDBiEOG091G02BL.
PhylomeDBiQ4VCS5.
TreeFamiTF333368.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4VCS5-1) [UniParc]FASTAAdd to basket
Also known as: p130

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS
60 70 80 90 100
GSGNPGPQSD VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM
110 120 130 140 150
QNNEELPTYE EAKVQSQYFR GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ
160 170 180 190 200
RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ MSLATSGVKA HPPVTSAPLS
210 220 230 240 250
PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP EYPFKGMPPQ
260 270 280 290 300
SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL
310 320 330 340 350
SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP
360 370 380 390 400
RPQQHFLPNQ AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ
410 420 430 440 450
QQPGEAYSAM PRAQPSSASY QPVPADPFAI VSRAQQMVEI LSDENRNLRQ
460 470 480 490 500
ELEGCYEKVA RLQKVETEIQ RVSEAYENLV KSSSKREALE KAMRNKLEGE
510 520 530 540 550
IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL FAKNKESQRE
560 570 580 590 600
KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV
610 620 630 640 650
DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT
660 670 680 690 700
NVSEYNAAAL MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA
710 720 730 740 750
TVAAQRDTTV ISHSPNTSYD TALEARIQKE EEEILMANKR CLDMEGRIKT
760 770 780 790 800
LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS CMRPAKSLMS ISNAGSGLLS
810 820 830 840 850
HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST PSPVPPSTPL
860 870 880 890 900
LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA
910 920 930 940 950
ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA
960 970 980 990 1000
ASVASAAAVA PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ
1010 1020 1030 1040 1050
TQAPTSAPAV APTPAPTPTP AVAQAEVPAS PATGPGPHRL SIPSLTCNPD
1060 1070 1080
KTDGPVFHSN TLERKTPIQI LGQEPDAEMV EYLI
Length:1,084
Mass (Da):118,085
Last modified:July 5, 2005 - v1
Checksum:iD7E7021E9535A628
GO
Isoform 2 (identifier: Q4VCS5-2) [UniParc]FASTAAdd to basket
Also known as: p80

The sequence of this isoform differs from the canonical sequence as follows:
     1-409: Missing.

Show »
Length:675
Mass (Da):72,540
Checksum:iEBC28B74427AD481
GO

Sequence cautioni

The sequence AAH94712 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0157091 – 409Missing in isoform 2. 2 PublicationsAdd BLAST409

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286598 mRNA. Translation: AAG01851.1.
AY987378 mRNA. Translation: AAY24451.1.
AB028994 mRNA. Translation: BAA83023.3.
BC094712 mRNA. Translation: AAH94712.1. Sequence problems.
CCDSiCCDS14563.1. [Q4VCS5-2]
CCDS48154.1. [Q4VCS5-1]
RefSeqiNP_001106962.1. NM_001113490.1. [Q4VCS5-1]
NP_573572.1. NM_133265.2. [Q4VCS5-2]
XP_005262144.1. XM_005262087.1. [Q4VCS5-1]
XP_005262147.1. XM_005262090.1. [Q4VCS5-2]
XP_011529177.1. XM_011530875.2. [Q4VCS5-1]
XP_016884778.1. XM_017029289.1. [Q4VCS5-2]
UniGeneiHs.528051.

Genome annotation databases

EnsembliENST00000304758; ENSP00000305557; ENSG00000126016. [Q4VCS5-2]
ENST00000371959; ENSP00000361027; ENSG00000126016. [Q4VCS5-1]
ENST00000524145; ENSP00000429013; ENSG00000126016. [Q4VCS5-1]
GeneIDi154796.
KEGGihsa:154796.
UCSCiuc004epr.4. human. [Q4VCS5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286598 mRNA. Translation: AAG01851.1.
AY987378 mRNA. Translation: AAY24451.1.
AB028994 mRNA. Translation: BAA83023.3.
BC094712 mRNA. Translation: AAH94712.1. Sequence problems.
CCDSiCCDS14563.1. [Q4VCS5-2]
CCDS48154.1. [Q4VCS5-1]
RefSeqiNP_001106962.1. NM_001113490.1. [Q4VCS5-1]
NP_573572.1. NM_133265.2. [Q4VCS5-2]
XP_005262144.1. XM_005262087.1. [Q4VCS5-1]
XP_005262147.1. XM_005262090.1. [Q4VCS5-2]
XP_011529177.1. XM_011530875.2. [Q4VCS5-1]
XP_016884778.1. XM_017029289.1. [Q4VCS5-2]
UniGeneiHs.528051.

3D structure databases

ProteinModelPortaliQ4VCS5.
SMRiQ4VCS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127557. 102 interactors.
DIPiDIP-53711N.
IntActiQ4VCS5. 79 interactors.
STRINGi9606.ENSP00000361027.

Chemistry databases

ChEMBLiCHEMBL3392949.

PTM databases

iPTMnetiQ4VCS5.
PhosphoSitePlusiQ4VCS5.

Polymorphism and mutation databases

BioMutaiAMOT.
DMDMi74753814.

Proteomic databases

EPDiQ4VCS5.
MaxQBiQ4VCS5.
PaxDbiQ4VCS5.
PeptideAtlasiQ4VCS5.
PRIDEiQ4VCS5.

Protocols and materials databases

DNASUi154796.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304758; ENSP00000305557; ENSG00000126016. [Q4VCS5-2]
ENST00000371959; ENSP00000361027; ENSG00000126016. [Q4VCS5-1]
ENST00000524145; ENSP00000429013; ENSG00000126016. [Q4VCS5-1]
GeneIDi154796.
KEGGihsa:154796.
UCSCiuc004epr.4. human. [Q4VCS5-1]

Organism-specific databases

CTDi154796.
DisGeNETi154796.
GeneCardsiAMOT.
HGNCiHGNC:17810. AMOT.
HPAiHPA067290.
MIMi300410. gene.
neXtProtiNX_Q4VCS5.
OpenTargetsiENSG00000126016.
PharmGKBiPA24773.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJU5. Eukaryota.
ENOG410XSY2. LUCA.
GeneTreeiENSGT00530000063846.
HOGENOMiHOG000233789.
HOVERGENiHBG066485.
InParanoidiQ4VCS5.
KOiK16819.
OMAiELDGCYE.
OrthoDBiEOG091G02BL.
PhylomeDBiQ4VCS5.
TreeFamiTF333368.

Enzyme and pathway databases

ReactomeiR-HSA-2028269. Signaling by Hippo.
SIGNORiQ4VCS5.

Miscellaneous databases

ChiTaRSiAMOT. human.
GeneWikiiAMOT.
GenomeRNAii154796.
PROiQ4VCS5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126016.
CleanExiHS_AMOT.
ExpressionAtlasiQ4VCS5. baseline and differential.
GenevisibleiQ4VCS5. HS.

Family and domain databases

InterProiIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PfamiPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSiPR01807. ANGIOMOTIN.
ProtoNetiSearch...

Entry informationi

Entry nameiAMOT_HUMAN
AccessioniPrimary (citable) accession number: Q4VCS5
Secondary accession number(s): Q504X5, Q9HD27, Q9UPT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2005
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Motus' means 'motility' in Latin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.