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Q4VCS5 (AMOT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Angiomotin
Gene names
Name:AMOT
Synonyms:KIAA1071
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1084 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Ref.1 Ref.6

Subunit structure

Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin. Ref.2 Ref.6

Subcellular location

Cell junctiontight junction. Note: Localized on the cell surface. May act as a transmembrane protein. Ref.2

Tissue specificity

Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta. Ref.1

Domain

The coiled coil domain interacts directly with the BAR domain of ARHGAP17. Ref.6

The angiostatin binding domain (871-1005) allows the binding to angiostatin. Ref.6

Post-translational modification

Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.

Miscellaneous

'Motus' means 'motility' in Latin.

Sequence similarities

Belongs to the angiomotin family.

Sequence caution

The sequence AAH94712.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
   Cellular componentCell junction
Tight junction
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Traceable author statement Ref.2. Source: UniProtKB

blood vessel endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

cell migration involved in gastrulation

Inferred from electronic annotation. Source: Ensembl

cell-cell junction assembly

Traceable author statement Ref.2. Source: UniProtKB

cellular protein localization

Inferred from direct assay PubMed 21205866. Source: MGI

chemotaxis

Inferred from electronic annotation. Source: Ensembl

establishment of cell polarity involved in ameboidal cell migration

Inferred from electronic annotation. Source: Ensembl

gastrulation with mouth forming second

Inferred from electronic annotation. Source: Ensembl

hippo signaling

Inferred from genetic interaction PubMed 21205866. Source: MGI

in utero embryonic development

Inferred from electronic annotation. Source: Ensembl

negative regulation of GTPase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of angiogenesis

Inferred from direct assay Ref.1. Source: MGI

negative regulation of vascular permeability

Inferred from direct assay Ref.2. Source: UniProtKB

positive regulation of blood vessel endothelial cell migration

Inferred from direct assay Ref.2. Source: UniProtKB

positive regulation of cell size

Traceable author statement Ref.2. Source: UniProtKB

positive regulation of embryonic development

Inferred from electronic annotation. Source: Ensembl

positive regulation of stress fiber assembly

Traceable author statement Ref.2. Source: UniProtKB

regulation of cell migration

Inferred from direct assay Ref.1. Source: MGI

regulation of small GTPase mediated signal transduction

Inferred from electronic annotation. Source: Ensembl

vasculogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentactin filament

Inferred from direct assay Ref.1. Source: UniProtKB

cell surface

Inferred from direct assay Ref.2. Source: UniProtKB

cytoplasm

Inferred from direct assay Ref.2. Source: UniProtKB

cytosol

Traceable author statement. Source: Reactome

endocytic vesicle

Inferred from direct assay Ref.1. Source: MGI

external side of plasma membrane

Inferred from direct assay Ref.1. Source: MGI

integral component of membrane

Inferred from direct assay Ref.2. Source: UniProtKB

lamellipodium

Inferred from direct assay Ref.1. Source: UniProtKB

ruffle

Inferred from direct assay Ref.1. Source: MGI

stress fiber

Inferred from direct assay Ref.2. Source: UniProtKB

tight junction

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functionangiostatin binding

Inferred from direct assay Ref.1Ref.2. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 19615732PubMed 21481793PubMed 23414517PubMed 24366813PubMed 21481793. Source: IntAct

receptor activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4VCS5-1)

Also known as: p130;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4VCS5-2)

Also known as: p80;

The sequence of this isoform differs from the canonical sequence as follows:
     1-409: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10841084Angiomotin
PRO_0000190668

Regions

Coiled coil429 – 689261 Potential
Coiled coil721 – 75131 Potential
Motif1081 – 10844PDZ-binding

Amino acid modifications

Modified residue7141Phosphoserine By similarity

Natural variations

Alternative sequence1 – 409409Missing in isoform 2.
VSP_015709

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (p130) [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: D7E7021E9535A628

FASTA1,084118,085
        10         20         30         40         50         60 
MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS GSGNPGPQSD 

        70         80         90        100        110        120 
VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM QNNEELPTYE EAKVQSQYFR 

       130        140        150        160        170        180 
GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ 

       190        200        210        220        230        240 
MSLATSGVKA HPPVTSAPLS PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP 

       250        260        270        280        290        300 
EYPFKGMPPQ SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL 

       310        320        330        340        350        360 
SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP RPQQHFLPNQ 

       370        380        390        400        410        420 
AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ QQPGEAYSAM PRAQPSSASY 

       430        440        450        460        470        480 
QPVPADPFAI VSRAQQMVEI LSDENRNLRQ ELEGCYEKVA RLQKVETEIQ RVSEAYENLV 

       490        500        510        520        530        540 
KSSSKREALE KAMRNKLEGE IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL 

       550        560        570        580        590        600 
FAKNKESQRE KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV 

       610        620        630        640        650        660 
DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT NVSEYNAAAL 

       670        680        690        700        710        720 
MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA TVAAQRDTTV ISHSPNTSYD 

       730        740        750        760        770        780 
TALEARIQKE EEEILMANKR CLDMEGRIKT LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS 

       790        800        810        820        830        840 
CMRPAKSLMS ISNAGSGLLS HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST 

       850        860        870        880        890        900 
PSPVPPSTPL LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA 

       910        920        930        940        950        960 
ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA ASVASAAAVA 

       970        980        990       1000       1010       1020 
PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ TQAPTSAPAV APTPAPTPTP 

      1030       1040       1050       1060       1070       1080 
AVAQAEVPAS PATGPGPHRL SIPSLTCNPD KTDGPVFHSN TLERKTPIQI LGQEPDAEMV 


EYLI 

« Hide

Isoform 2 (p80) [UniParc].

Checksum: EBC28B74427AD481
Show »

FASTA67572,540

References

« Hide 'large scale' references
[1]"Angiomotin. An angiostatin binding protein that regulates endothelial cell migration and tube formation."
Troyanovsky B., Levchenko T., Maensson G., Matvijenko O., Holmgren L.
J. Cell Biol. 152:1247-1254(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION.
Tissue: Placenta.
[2]"Angiomotin regulates endothelial cell-cell junctions and cell motility."
Bratt A., Birot O., Sinha I., Veitonmaeki N., Aase K., Ernkvist M., Holmgren L.
J. Biol. Chem. 280:34859-34869(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TOPOLOGY, INTERACTION WITH ANGIOSTATIN AND MAGI1, SUBCELLULAR LOCATION.
[3]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-543 (ISOFORM 1).
Tissue: Spinal cord.
[6]"A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells."
Wells C.D., Fawcett J.P., Traweger A., Yamanaka Y., Goudreault M., Elder K., Kulkarni S., Gish G., Virag C., Lim C., Colwill K., Starostine A., Metalnikov P., Pawson T.
Cell 125:535-548(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN, FUNCTION, IDENTIFICATION IN A COMPLEX WITH ARHGAP17; MPP5; INADL AND PARD3.
[7]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"The Nedd4-like ubiquitin E3 ligases target angiomotin/p130 to ubiquitin-dependent degradation."
Wang C., An J., Zhang P., Xu C., Gao K., Wu D., Wang D., Yu H., Liu J.O., Yu L.
Biochem. J. 444:279-289(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION BY NEDD4; NEDD4L AND ITCH.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF286598 mRNA. Translation: AAG01851.1.
AY987378 mRNA. Translation: AAY24451.1.
AB028994 mRNA. Translation: BAA83023.3.
BC094712 mRNA. Translation: AAH94712.1. Sequence problems.
CCDSCCDS14563.1. [Q4VCS5-2]
CCDS48154.1. [Q4VCS5-1]
RefSeqNP_001106962.1. NM_001113490.1. [Q4VCS5-1]
NP_573572.1. NM_133265.2. [Q4VCS5-2]
XP_005262144.1. XM_005262087.1. [Q4VCS5-1]
XP_005262145.1. XM_005262088.1. [Q4VCS5-1]
XP_005262146.1. XM_005262089.1. [Q4VCS5-1]
XP_005262147.1. XM_005262090.1. [Q4VCS5-2]
XP_006724686.1. XM_006724623.1. [Q4VCS5-2]
UniGeneHs.528051.

3D structure databases

ProteinModelPortalQ4VCS5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127557. 62 interactions.
IntActQ4VCS5. 60 interactions.
STRING9606.ENSP00000361027.

PTM databases

PhosphoSiteQ4VCS5.

Polymorphism databases

DMDM74753814.

Proteomic databases

MaxQBQ4VCS5.
PaxDbQ4VCS5.
PRIDEQ4VCS5.

Protocols and materials databases

DNASU154796.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000304758; ENSP00000305557; ENSG00000126016. [Q4VCS5-2]
ENST00000371959; ENSP00000361027; ENSG00000126016. [Q4VCS5-1]
ENST00000524145; ENSP00000429013; ENSG00000126016. [Q4VCS5-1]
GeneID154796.
KEGGhsa:154796.
UCSCuc004epr.3. human. [Q4VCS5-1]

Organism-specific databases

CTD154796.
GeneCardsGC0XM112017.
HGNCHGNC:17810. AMOT.
MIM300410. gene.
neXtProtNX_Q4VCS5.
PharmGKBPA24773.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG131728.
HOGENOMHOG000233789.
HOVERGENHBG066485.
InParanoidQ4VCS5.
KOK16819.
OMAPGKVHQD.
OrthoDBEOG7M6D6Q.
PhylomeDBQ4VCS5.
TreeFamTF333368.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ4VCS5.
BgeeQ4VCS5.
CleanExHS_AMOT.
GenevestigatorQ4VCS5.

Family and domain databases

InterProIPR009114. Angiomotin.
IPR024646. Angiomotin_C.
[Graphical view]
PANTHERPTHR14826. PTHR14826. 1 hit.
PfamPF12240. Angiomotin_C. 1 hit.
[Graphical view]
PRINTSPR01807. ANGIOMOTIN.
ProtoNetSearch...

Other

ChiTaRSAMOT. human.
GeneWikiAMOT.
GenomeRNAi154796.
NextBio87314.
PROQ4VCS5.
SOURCESearch...

Entry information

Entry nameAMOT_HUMAN
AccessionPrimary (citable) accession number: Q4VCS5
Secondary accession number(s): Q504X5, Q9HD27, Q9UPT1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM