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Protein

Methionine aminopeptidase 1D, mitochondrial

Gene

metap1d

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateUniRule annotation1
Metal bindingi181Divalent metal cation 1UniRule annotation1
Metal bindingi192Divalent metal cation 1UniRule annotation1
Metal bindingi192Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi255Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei262SubstrateUniRule annotation1
Metal bindingi287Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi318Divalent metal cation 1UniRule annotation1
Metal bindingi318Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.001

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1D, mitochondrialUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 1DUniRule annotation
Short name:
MetAP 1DUniRule annotation
Alternative name(s):
Methionyl aminopeptidase type 1D, mitochondrial
Peptidase M 1DUniRule annotation
Gene namesi
Name:metap1d
Synonyms:map1d
ORF Names:zgc:110461
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi

Organism-specific databases

ZFINiZDB-GENE-050522-71 metap1d

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionUniRule annotationAdd BLAST47
ChainiPRO_000031412848 – 338Methionine aminopeptidase 1D, mitochondrialAdd BLAST291

Proteomic databases

PaxDbiQ4VBS4
PRIDEiQ4VBS4

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000010471

Structurei

3D structure databases

ProteinModelPortaliQ4VBS4
SMRiQ4VBS4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2738 Eukaryota
COG0024 LUCA
HOGENOMiHOG000030427
HOVERGENiHBG067178
InParanoidiQ4VBS4
KOiK01265
PhylomeDBiQ4VBS4

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4VBS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPCAAQCL YRTGGLRLLQ RISRLPHCHK DASLAHQCQF HRSFFWRKPK
60 70 80 90 100
TSHSVVRPAI VRPAYPVPKH IQRPDYVSSS KVPEWPDYIE IKDEEQIQGL
110 120 130 140 150
RRACQLARHI LLLTGNSLKV GMTTDEIDFI VHQEAIRHNG YPSPLHYGGF
160 170 180 190 200
PKSVCTSVNN VVCHGIPDSR PLQDGDIINI DVTVYLEGYH GDTSETFLIG
210 220 230 240 250
SVNDQGRKLV DVARQCRDQA IAACGPGQPL CVIGNIISNI ANSNGFRVCP
260 270 280 290 300
YFIGHGIGEY FHGHPEIWHH ANDNDLKMEE GMSFTIEPIL MEGTSGFRIL
310 320 330
SDKWTAVSVD DKRSAQFEHT VVITSDGVEI LTKLPEED
Length:338
Mass (Da):37,785
Last modified:July 5, 2005 - v1
Checksum:i4FB31183518AD263
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC095283 mRNA Translation: AAH95283.1
RefSeqiNP_001019563.1, NM_001024392.1
UniGeneiDr.42917

Genome annotation databases

GeneIDi554090
KEGGidre:554090

Similar proteinsi

Entry informationi

Entry nameiMAP12_DANRE
AccessioniPrimary (citable) accession number: Q4VBS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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