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Q4VBH4

- UB2WA_DANRE

UniProt

Q4VBH4 - UB2WA_DANRE

Protein

Probable ubiquitin-conjugating enzyme E2 W-A

Gene

ube2wa

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to misfolded protein Source: UniProtKB
    2. DNA repair Source: UniProtKB-KW
    3. misfolded or incompletely synthesized protein catabolic process Source: UniProtKB
    4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
    5. protein monoubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin-conjugating enzyme E2 W-A (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein W-A
    Ubiquitin-protein ligase W-A
    Gene namesi
    Name:ube2wa
    Synonyms:ube2w
    ORF Names:im:6905459, si:ch211-193n21.4
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 2

    Organism-specific databases

    ZFINiZDB-GENE-050506-94. ube2w.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 151151Probable ubiquitin-conjugating enzyme E2 W-APRO_0000232691Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ4VBH4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4VBH4.
    SMRiQ4VBH4. Positions 1-117.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110854.
    HOGENOMiHOG000233453.
    HOVERGENiHBG063308.
    InParanoidiQ4VBH4.
    OMAiQRWVIEA.
    OrthoDBiEOG7CG71H.
    PhylomeDBiQ4VBH4.
    TreeFamiTF314582.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4VBH4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASMQKRLQK ELLALQNDPP AGMTLNERSV QNTITEWFID MEGAQGTVYE    50
    GEKFQLLFKF SSRYPFESPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS 100
    PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVKTCNKNPK KTKWWYHDDT 150
    C 151
    Length:151
    Mass (Da):17,399
    Last modified:April 18, 2006 - v2
    Checksum:i6AB7228D0D8FC242
    GO

    Sequence cautioni

    The sequence AAH95816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT027743 Genomic DNA. Translation: CAQ15478.1.
    BC095816 mRNA. Translation: AAH95816.1. Different initiation.
    RefSeqiNP_001116444.1. NM_001122972.1.
    UniGeneiDr.88650.

    Genome annotation databases

    EnsembliENSDART00000130142; ENSDARP00000108827; ENSDARG00000044923.
    GeneIDi553013.
    KEGGidre:553013.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT027743 Genomic DNA. Translation: CAQ15478.1 .
    BC095816 mRNA. Translation: AAH95816.1 . Different initiation.
    RefSeqi NP_001116444.1. NM_001122972.1.
    UniGenei Dr.88650.

    3D structure databases

    ProteinModelPortali Q4VBH4.
    SMRi Q4VBH4. Positions 1-117.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000130142 ; ENSDARP00000108827 ; ENSDARG00000044923 .
    GeneIDi 553013.
    KEGGi dre:553013.

    Organism-specific databases

    CTDi 55284.
    ZFINi ZDB-GENE-050506-94. ube2w.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110854.
    HOGENOMi HOG000233453.
    HOVERGENi HBG063308.
    InParanoidi Q4VBH4.
    OMAi QRWVIEA.
    OrthoDBi EOG7CG71H.
    PhylomeDBi Q4VBH4.
    TreeFami TF314582.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 20879916.

    Gene expression databases

    Bgeei Q4VBH4.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.

    Entry informationi

    Entry nameiUB2WA_DANRE
    AccessioniPrimary (citable) accession number: Q4VBH4
    Secondary accession number(s): B0V2U1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3