Q4VBH4 (UB2WA_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ubiquitin-conjugating enzyme E2 W-A
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein W-A
Ubiquitin-protein ligase W-A

Gene names

Name:	ube2wa
Synonyms:	ube2w
ORF Names:	im:6905459, si:ch211-193n21.4

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAH95816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cellular response to misfolded protein Inferred from sequence or structural similarity. Source: UniProtKB misfolded or incompletely synthesized protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein monoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Probable ubiquitin-conjugating enzyme E2 W-A		PRO_0000232691
Sites
	Active site	91	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4VBH4 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 6AB7228D0D8FC242	FASTA	151	17,399
	10 20 30 40 50 60 MASMQKRLQK ELLALQNDPP AGMTLNERSV QNTITEWFID MEGAQGTVYE GEKFQLLFKF 70 80 90 100 110 120 SSRYPFESPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK 130 140 150 EKRRPPDNSF YVKTCNKNPK KTKWWYHDDT C

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT027743 Genomic DNA. Translation: CAQ15478.1. BC095816 mRNA. Translation: AAH95816.1. Different initiation.
IPI	IPI00608620.
RefSeq	NP_001116444.1. NM_001122972.1.
UniGene	Dr.88650.
3D structure databases
ProteinModelPortal	Q4VBH4.
SMR	Q4VBH4. Positions 1-117.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000130142; ENSDARP00000108827; ENSDARG00000044923.
GeneID	553013.
KEGG	dre:553013.
Organism-specific databases
CTD	55284.
ZFIN	ZDB-GENE-050506-94. ube2w.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00700000104488.
HOGENOM	HOG000233453.
HOVERGEN	HBG063308.
InParanoid	Q4VBH4.
OrthoDB	EOG49CQ8W.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q4VBH4.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20879916.

Entry information

Entry name

UB2WA_DANRE

Accession

Primary (citable) accession number: Q4VBH4
Secondary accession number(s): B0V2U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	April 18, 2006
Last modified:	March 6, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program