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Protein

Probable ubiquitin-conjugating enzyme E2 W-A

Gene

ube2wa

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-DRE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin-conjugating enzyme E2 W-A (EC:2.3.2.25)
Alternative name(s):
E2 ubiquitin-conjugating enzyme W-A
N-terminal E2 ubiquitin-conjugating enzyme (EC:2.3.2.23)
Ubiquitin carrier protein W-A
Ubiquitin-protein ligase W-A
Gene namesi
Name:ube2wa
Synonyms:ube2w
ORF Names:im:6905459, si:ch211-193n21.4
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 2

Organism-specific databases

ZFINiZDB-GENE-050506-94 ube2w

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326911 – 151Probable ubiquitin-conjugating enzyme E2 W-AAdd BLAST151

Proteomic databases

PaxDbiQ4VBH4

Expressioni

Gene expression databases

BgeeiENSDARG00000044923

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000108827

Structurei

3D structure databases

ProteinModelPortaliQ4VBH4
SMRiQ4VBH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0427 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00910000143990
HOGENOMiHOG000233453
HOVERGENiHBG063308
InParanoidiQ4VBH4
KOiK10688
OMAiEDWTPAC
OrthoDBiEOG091G0RCH
PhylomeDBiQ4VBH4
TreeFamiTF314582

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q4VBH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMQKRLQK ELLALQNDPP AGMTLNERSV QNTITEWFID MEGAQGTVYE
60 70 80 90 100
GEKFQLLFKF SSRYPFESPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS
110 120 130 140 150
PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVKTCNKNPK KTKWWYHDDT

C
Length:151
Mass (Da):17,399
Last modified:April 18, 2006 - v2
Checksum:i6AB7228D0D8FC242
GO

Sequence cautioni

The sequence AAH95816 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT027743 Genomic DNA Translation: CAQ15478.1
BC095816 mRNA Translation: AAH95816.1 Different initiation.
RefSeqiNP_001116444.1, NM_001122972.1
UniGeneiDr.88650

Genome annotation databases

EnsembliENSDART00000130142; ENSDARP00000108827; ENSDARG00000044923
GeneIDi553013
KEGGidre:553013

Similar proteinsi

Entry informationi

Entry nameiUB2WA_DANRE
AccessioniPrimary (citable) accession number: Q4VBH4
Secondary accession number(s): B0V2U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: May 23, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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