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Protein

Pikachurin

Gene

Egflam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.2 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • glycosaminoglycan binding Source: MGI

GO - Biological processi

  • extracellular matrix organization Source: MGI
  • peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan Source: MGI
  • positive regulation of cell-substrate adhesion Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pikachurin
Alternative name(s):
EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Nectican
Gene namesi
Name:Egflam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146149. Egflam.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cell junction Source: UniProtKB-KW
  • cell surface Source: MGI
  • interstitial matrix Source: MGI
  • proteinaceous extracellular matrix Source: MGI
  • synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Extracellular matrix, Secreted, Synapse

Pathology & Biotechi

Disruption phenotypei

No morphological abnormalities. Knockout mice are viable and fertile. However, the terminal of bipolar cells do not appose to the synapse terminals in the rod photoreceptor ribbon synapses. The signal transmission from the rod photoreceptor to the rod bipolar cells is less sensitive and is delayed compared to the wild type mouse. The signal transmission from cone photoreceptors to the cone bipolar cells is also impaired. These mice show reduced visual function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000030680425 – 1017PikachurinAdd BLAST993

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi347 ↔ 358By similarity
Disulfide bondi352 ↔ 369By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi534 ↔ 564By similarity
Disulfide bondi569 ↔ 580By similarity
Disulfide bondi574 ↔ 590By similarity
Disulfide bondi592 ↔ 601By similarity
Disulfide bondi788 ↔ 799By similarity
Disulfide bondi793 ↔ 808By similarity
Disulfide bondi810 ↔ 819By similarity
Disulfide bondi987 ↔ 1014By similarity

Post-translational modificationi

O-glycosylated; contains chondroitin sulfate and heparan sulfate.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ4VBE4.
PRIDEiQ4VBE4.

PTM databases

PhosphoSitePlusiQ4VBE4.

Expressioni

Tissue specificityi

Expressed in the outer plexiform layer (first synaptic region) but not in the inner plexiform layer (second synaptic region) of the retina (at protein level). Strongly expressed in the photoreceptor layer of the retina. Moderately expressed in pineal gland and brain. Weakly expressed in lung and ovary.1 Publication

Developmental stagei

Expressed in the apical side of the neuroblastic layer (NBL) of the retina at E14.5 and E17.5. At E16.5, present in rib cartilage and hair follicle (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000042961.
ExpressionAtlasiQ4VBE4. baseline and differential.
GenevisibleiQ4VBE4. MM.

Interactioni

Subunit structurei

Interacts with DAG1 alpha-dystroglycan.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dag1Q621652EBI-2025048,EBI-2025154

Protein-protein interaction databases

IntActiQ4VBE4. 4 interactors.
STRINGi10090.ENSMUSP00000055599.

Structurei

3D structure databases

ProteinModelPortaliQ4VBE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 136Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini144 – 239Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini343 – 381EGF-like 1PROSITE-ProRule annotationAdd BLAST39
Domaini386 – 564Laminin G-like 1PROSITE-ProRule annotationAdd BLAST179
Domaini565 – 602EGF-like 2PROSITE-ProRule annotationAdd BLAST38
Domaini609 – 788Laminin G-like 2PROSITE-ProRule annotationAdd BLAST180
Domaini784 – 820EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini835 – 1014Laminin G-like 3PROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 3 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XRPX. LUCA.
GeneTreeiENSGT00530000063501.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiQ4VBE4.
OMAiEIALHTN.
OrthoDBiEOG091G048M.
PhylomeDBiQ4VBE4.
TreeFamiTF326548.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 1 hit.
PF02210. Laminin_G_2. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4VBE4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLISTFSLH FLLLACSLPP GAVSLRTALR KSGKVGPPLD IKLGALNCTA
60 70 80 90 100
FSIQWKTPKR SGSSIIGYTV FYSEVGSDKS LRERSHNVPV GQDTLITEEV
110 120 130 140 150
IGDLKPGTEY QVSVAAYSQT GKGRLSFPRH VTTLSQDSCL PPAAPQQPHV
160 170 180 190 200
LVVSDSEVAL SWRPGENEGS APIQSYSVEF IRPDFDKSWT IIQERLQMDS
210 220 230 240 250
MVIKGLDPDT NYQFAVKAMN AHGFSPRSWP SNTVRTLGPG EAGSGHYGPG
260 270 280 290 300
YITNPGVSED DDGSEDELDL DVSFEEVKPL PATKVGNKKF SVESKKTSVS
310 320 330 340 350
NSVMGSRLAQ PTSASLHETT VAIPPTPAQR KGKNSVAMMS RLFDMSCDET
360 370 380 390 400
LCSADSFCVN DYAWGGSRCH CNLGKGGEAC SEDIFIQYPQ FFGHSYVTFE
410 420 430 440 450
PLKNSYQAFQ VTLEFRAEAE DGLLLYCGES EHGRGDFMSL ALIRRSLHFR
460 470 480 490 500
FNCGTGIAII ISETKIKLGA WHTVTLYRDG LNGMLQLNNG TPVTGQSQGQ
510 520 530 540 550
YSKITFRTPL YLGGAPSAYW LVRATGTNRG FQGCVQSLSV NGKKIDMRPW
560 570 580 590 600
PLGKALNGAD VGECSSGICD EASCIHGGTC AAIKADSYIC LCPLGFRGRH
610 620 630 640 650
CEDAFALTIP QFRESLRSYA ATPWPLEPQH YLSFTEFEIT FRPDSGDGVL
660 670 680 690 700
LYSYDTGSKD FLSINMAAGH VEFRFDCGSG TGVLRSEAPL TLGQWHDLRV
710 720 730 740 750
SRTAKNGILQ VDKQKVVEGM AEGGFTQIKC NTDIFIGGVP NYDDVKKNSG
760 770 780 790 800
ILHPFSGSIQ KIILNDRTIH VKHDFTSGVN VENAAHPCVG APCAHGGSCR
810 820 830 840 850
PRKEGYECDC PLGFEGLNCQ KECGNHCLNT IIEAIEIPQF IGRSYLTYDN
860 870 880 890 900
PNILKRVSGS RSNAFMRFKT TAKDGLLLWR GDSPMRPNSD FISLGLRDGA
910 920 930 940 950
LIFSYNLGSG VASIMVNGSF SDGRWHRVKA VRDGQSGKIT VDDYGARTGK
960 970 980 990 1000
SPGLMRQLNI NGALYVGGMK EIALHTNRQY LRGLVGCISH FTLSTDYHIS
1010
LVEDAVDGKN INTCGAK
Length:1,017
Mass (Da):110,735
Last modified:July 5, 2005 - v1
Checksum:iF8A21D89B251D455
GO
Isoform 2 (identifier: Q4VBE4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     822-830: ECGNHCLNT → A

Show »
Length:1,009
Mass (Da):109,834
Checksum:i80DD954F255C102B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45A → T in AAI50711 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028482822 – 830ECGNHCLNT → A in isoform 2. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ223720 mRNA. Translation: ABB48431.1.
AK031136 mRNA. Translation: BAC27271.1.
AK033332 mRNA. Translation: BAC28235.1.
AK037223 mRNA. Translation: BAC29762.1.
AK041546 mRNA. Translation: BAC30982.1.
AC105969 Genomic DNA. No translation available.
AC158747 Genomic DNA. No translation available.
BC051455 mRNA. Translation: AAH51455.1.
BC095994 mRNA. Translation: AAH95994.1.
BC150710 mRNA. Translation: AAI50711.1.
CCDSiCCDS27370.1. [Q4VBE4-2]
CCDS79359.1. [Q4VBE4-1]
RefSeqiNP_001276425.1. NM_001289496.1. [Q4VBE4-1]
NP_848863.1. NM_178748.6. [Q4VBE4-2]
XP_017172117.1. XM_017316628.1. [Q4VBE4-2]
UniGeneiMm.203208.

Genome annotation databases

EnsembliENSMUST00000058593; ENSMUSP00000055599; ENSMUSG00000042961. [Q4VBE4-2]
ENSMUST00000096494; ENSMUSP00000094238; ENSMUSG00000042961. [Q4VBE4-1]
GeneIDi268780.
KEGGimmu:268780.
UCSCiuc007vea.2. mouse. [Q4VBE4-2]
uc007veb.2. mouse. [Q4VBE4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ223720 mRNA. Translation: ABB48431.1.
AK031136 mRNA. Translation: BAC27271.1.
AK033332 mRNA. Translation: BAC28235.1.
AK037223 mRNA. Translation: BAC29762.1.
AK041546 mRNA. Translation: BAC30982.1.
AC105969 Genomic DNA. No translation available.
AC158747 Genomic DNA. No translation available.
BC051455 mRNA. Translation: AAH51455.1.
BC095994 mRNA. Translation: AAH95994.1.
BC150710 mRNA. Translation: AAI50711.1.
CCDSiCCDS27370.1. [Q4VBE4-2]
CCDS79359.1. [Q4VBE4-1]
RefSeqiNP_001276425.1. NM_001289496.1. [Q4VBE4-1]
NP_848863.1. NM_178748.6. [Q4VBE4-2]
XP_017172117.1. XM_017316628.1. [Q4VBE4-2]
UniGeneiMm.203208.

3D structure databases

ProteinModelPortaliQ4VBE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4VBE4. 4 interactors.
STRINGi10090.ENSMUSP00000055599.

PTM databases

PhosphoSitePlusiQ4VBE4.

Proteomic databases

PaxDbiQ4VBE4.
PRIDEiQ4VBE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058593; ENSMUSP00000055599; ENSMUSG00000042961. [Q4VBE4-2]
ENSMUST00000096494; ENSMUSP00000094238; ENSMUSG00000042961. [Q4VBE4-1]
GeneIDi268780.
KEGGimmu:268780.
UCSCiuc007vea.2. mouse. [Q4VBE4-2]
uc007veb.2. mouse. [Q4VBE4-1]

Organism-specific databases

CTDi133584.
MGIiMGI:2146149. Egflam.

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XRPX. LUCA.
GeneTreeiENSGT00530000063501.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiQ4VBE4.
OMAiEIALHTN.
OrthoDBiEOG091G048M.
PhylomeDBiQ4VBE4.
TreeFamiTF326548.

Miscellaneous databases

PROiQ4VBE4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042961.
ExpressionAtlasiQ4VBE4. baseline and differential.
GenevisibleiQ4VBE4. MM.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 1 hit.
PF02210. Laminin_G_2. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEGFLA_MOUSE
AccessioniPrimary (citable) accession number: Q4VBE4
Secondary accession number(s): B2RWU6
, B6JU28, Q80WX4, Q8BGP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: July 5, 2005
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.