Reviewed, UniProtKB/Swiss-Prot Q4VA72 (BRCC3_XENTR)

Names and origin

Protein names

Recommended name:
    Lys-63-specific deubiquitinase BRCC36
    EC=3.1.2.15
Alternative name(s):
    BRCA1-A complex subunit BRCC36
    BRISC complex subunit BRCC36
    BRCA1/BRCA2-containing complex subunit 3
    BRCA1/BRCA2-containing complex subunit 36

Gene names

Name:	brcc3
Synonyms:	brcc36
ORF Names:	TEgg028n11.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the brca1-bard1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the rnf8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex By similarity.
Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Subunit structure	Component of the BRCA1-A complex. Component of the BRISC complex By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs) By similarity.
Sequence similarities	Belongs to the peptidase M67A family. BRCC36 subfamily. Contains 1 MPN (JAB/Mov34) domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	G2/M transition DNA damage checkpoint Inferred from sequence or structural similarity. Source: UniProtKB double-strand break repair Inferred from sequence or structural similarity. Source: UniProtKB histone H2A K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of DNA repair Inferred from sequence or structural similarity. Source: UniProtKB response to ionizing radiation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	BRCA1-A complex Inferred from sequence or structural similarity. Source: UniProtKB BRISC complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	metallopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB polyubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Lys-63-specific deubiquitinase BRCC36		PRO_0000373950
Regions
	Motif	92 – 105	14	JAMM motif
Sites
	Metal binding	92	1	Zinc; catalytic By similarity
	Metal binding	94	1	Zinc; catalytic By similarity
	Metal binding	105	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4VA72-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 76552F868C87FAAF	FASTA	261	29,719
	10 20 30 40 50 60 MAVQAVHIQG DAFLVCVTHS LSTEREEVMG LCIGEVDTQK VVHIHSVIIL RRSDKRKDRV 70 80 90 100 110 120 EISPEQLSAA TTEADRLAEI TGRPMRVVGW YHSHPHITVW PSHVDVRTQA MYQMMDVGFV 130 140 150 160 170 180 GLIFSCFIED KNTKTGRILY TCFQSVQAQK SSEYERIEVP LHVVPHNTIR KVCLESAVEL 190 200 210 220 230 240 PRILCQEEQD AYRRIHSLGH LDSITKIHNG SVFTKNLCGQ MSAISGPLLQ WLEDRLEQNQ 250 260 QRAQELQSEK EQLLQELKAL G

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR760965 mRNA. Translation: CAJ82080.1. BC096514 mRNA. Translation: AAH96514.1.
RefSeq	NP_001016457.1.
UniGene	Str.51157
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M67.004.
Genome annotation databases
Ensembl	ENSXETT00000055343; ENSXETP00000055343; ENSXETG00000026184; Xenopus tropicalis. [Genome view] ENSXETT00000055344; ENSXETP00000055344; ENSXETG00000026184; Xenopus tropicalis. [Genome view]
GeneID	549211.
KEGG	xtr:549211.
Organism-specific databases
CTD	549211.
Xenbase	XB-FEAT-5812884. brcc3.
Phylogenomic databases
HOGENOM	Q4VA72.
HOVERGEN	Q4VA72.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
ProDom	PD363422. Mov34-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRCC3_XENTR

Accession

Primary (citable) accession number: Q4VA72

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 5, 2005
Last modified:	September 1, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project