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Q4VA53 (PDS5B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sister chromatid cohesion protein PDS5 homolog B
Alternative name(s):
Androgen-induced proliferation inhibitor
Androgen-induced prostate proliferative shutoff-associated protein AS3
Gene names
Name:Pds5b
Synonyms:Aprin, As3, Kiaa0979
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells By similarity.

Subunit structure

Interacts with the cohesin complex. Interacts with RAD21; the interaction is direct. Interacts with WAPAL (via FGF motifs) or CDCA5 (via the FGF motif); the interaction is direct, cohesin-dependent and competitive By similarity.

Subcellular location

Nucleus By similarity UniProtKB Q6TRW4.

Tissue specificity

Expressed in prostate. Ref.1

Sequence similarities

Belongs to the PDS5 family.

Contains 3 A.T hook DNA-binding domains.

Contains 1 HEAT repeat.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitotic sister chromatid cohesion

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentchromatin

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q4VA53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.2 (identifier: Q4VA53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-529: Missing.
     1207-1230: SELEKPRSRKKAPVTDPEEKLGMD → VRVRCLVGRVMRLLIVIVLVIFAL
     1231-1446: Missing.
Note: No experimental confirmation available.
Isoform 3 Ref.2 (identifier: Q4VA53-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1206-1206: L → LVR
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14461446Sister chromatid cohesion protein PDS5 homolog B
PRO_0000287425

Regions

Repeat383 – 41937HEAT
DNA binding1247 – 125913A.T hook 1
DNA binding1285 – 129713A.T hook 2
DNA binding1370 – 138213A.T hook 3

Amino acid modifications

Modified residue11361N6-acetyllysine Ref.9
Modified residue11621Phosphoserine By similarity
Modified residue11661Phosphoserine Ref.7 UniProtKB Q9NTI5
Modified residue11761Phosphoserine By similarity
Modified residue11821Phosphoserine By similarity
Modified residue11911Phosphoserine By similarity
Modified residue12531Phosphothreonine Ref.7
Modified residue12551Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue12571Phosphoserine Ref.7
Modified residue12811Phosphoserine Ref.7
Modified residue13561Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue13651Phosphothreonine By similarity
Modified residue13681Phosphothreonine By similarity UniProtKB Q9NTI5
Modified residue13771Phosphoserine By similarity UniProtKB Q9NTI5
Modified residue13811Phosphoserine By similarity UniProtKB Q9NTI5

Natural variations

Alternative sequence1 – 529529Missing in isoform 2. Ref.2
VSP_052403
Alternative sequence12061L → LVR in isoform 3. Ref.2
VSP_052404
Alternative sequence1207 – 123024SELEK…KLGMD → VRVRCLVGRVMRLLIVIVLV IFAL in isoform 2. Ref.2
VSP_052405
Alternative sequence1231 – 1446216Missing in isoform 2. Ref.2
VSP_052406

Experimental info

Sequence conflict4961W → R in AAM52216. Ref.1
Sequence conflict9661I → T in AAM52216. Ref.1
Sequence conflict9781A → S in AAM52216. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 3540041DE3C7FCBE

FASTA1,446164,419
        10         20         30         40         50         60 
MAHSKTRTND GKITYPPGVK EISDKISKEE MVRRLKMVVK TFMDMDQDSE EEKELYLNLA 

        70         80         90        100        110        120 
LHLASDFFLK HPDKDVRLLV ACCLADIFRI YAPEAPYTSP DKLKDIFMFI TRQLKGLEDT 

       130        140        150        160        170        180 
KSPQFNRYFY LLENIAWVKS YNICFELEDS NEIFTQLYRT LFSVINNGHN QKVHMHMVDL 

       190        200        210        220        230        240 
MSSIICEGDT VSQELLDTVL VNLVPAHKNL NKQAYDLAKA LLKRTAQAIE PYITNFFNQV 

       250        260        270        280        290        300 
LMLGKTSISD LSEHVFDLIL ELYNIDSHLL LSVLPQLEFK LKSNDNEERL QVVKLLAKMF 

       310        320        330        340        350        360 
GAKDSELASQ NKPLWQCYLG RFNDIHVPIR LECVKFASHC LMNHPDLAKD LTEYLKVRSH 

       370        380        390        400        410        420 
DPEEAIRHDV IVSIVTAAKK DILLVNDHLL NFVRERTLDK RWRVRKEAMM GLAQIYKKYS 

       430        440        450        460        470        480 
LQSAAGKDAA KQISWVKDKL LHIYYQNSID DRLLVERIFA QYMVPHNLET TERMKCLYYL 

       490        500        510        520        530        540 
YATLDLNAVK ALNEMWKCQN LLRHQVKDLL DLIKQPKTDA SVKAIFSKVM VITRNLPDPG 

       550        560        570        580        590        600 
KAQDFMKKFT QVLEDDEKIR KQLEALVSPT CSCKQAEGCV REITKKLGNP KQPTNPFLEM 

       610        620        630        640        650        660 
IKFLLERIAP VHIDTESISA LIKQVNKSID GTADDEDEGV PTDQAIRAGL ELLKVLSFTH 

       670        680        690        700        710        720 
PISFHSAETF ESLLACLKMD DEKVAEAALQ IFKNTGSKIE EDFPHIRSAL LPVLHHKSKK 

       730        740        750        760        770        780 
GPPRQAKYAI HCIHAIFSSK ETQFAQIFEP LHKSLDPSNL EHLITPLVTI GHIALLAPDQ 

       790        800        810        820        830        840 
FAAPLKSLVA TFIVKDLLMN DRLPGKKTTK LWVPDEEVSP ETMVKIQAIK MMVRWLLGMK 

       850        860        870        880        890        900 
NNHSKSGTST LRLLTTILHS DGDLTEQGKI SKPDMSRLRL AAGSAIVKLA QEPCYHEIIT 

       910        920        930        940        950        960 
LEQYQLCALA INDECYQVRQ VFAQKLHKGL SRLRLPLEYM AICALCAKDP VKERRAHARQ 

       970        980        990       1000       1010       1020 
CLVKNITVRR EYLKQHAAVS EKLLSLLPEY VVPYTIHLLA HDPDYVKVQD IEQLKDVKEC 

      1030       1040       1050       1060       1070       1080 
LWFVLEILMA KNENNSHAFI RKMVENIKQT KDAQGPDDTK MNEKLYTVCD VAMNIIMSKS 

      1090       1100       1110       1120       1130       1140 
TTYSLESPKD PVLPARFFTQ PDKNFSNTKN YLPPEMKSFF TPGKPKTANV LGAVNKPLSS 

      1150       1160       1170       1180       1190       1200 
AGKQSQTKSS RMETVSNASS SSNPSSPGRI KGRLDSSEMD HSENEDYTMS SPLPGKKSDK 

      1210       1220       1230       1240       1250       1260 
REDPDLSELE KPRSRKKAPV TDPEEKLGMD DLTKLVQEQK PKGSQRGRKR GRTASDSDEQ 

      1270       1280       1290       1300       1310       1320 
QWPEEKRHKE ELLENEDEQN SPPKKGKRGR PPKPLGGGTS KEEPTMKTSK KGNKKKLVPP 

      1330       1340       1350       1360       1370       1380 
VVDDDEEEER QIGNTEHKSK SKQHRTSKRA QQRAESPETS AVESTQSTPQ KGRGRPSKAP 

      1390       1400       1410       1420       1430       1440 
SPSQPPKKIR VGRSKQVATK ENDSSEEMDV LQASSPVSDD TTQEGAEEED ISVGNVRRRS 


SKRERR 

« Hide

Isoform 2 [UniParc].

Checksum: FAF6F0351AF7869B
Show »

FASTA70178,714
Isoform 3 [UniParc].

Checksum: 80CFDE441169F45E
Show »

FASTA1,448164,674

References

« Hide 'large scale' references
[1]"The AS3 proliferative arrest gene has an ancient eukaryotic heritage and shows highly conserved functional domains in mice."
Geck P., Maffini M., Sonnenschein C., Soto A.M.
Proc. Annu. Meet. Am. Assoc. Cancer Res. 43:987-987(2002)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 625-1446 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1092-1446 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cerebellum, Embryo, Head and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 256-1446 (ISOFORM 1).
Tissue: Brain.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1166; THR-1253; SER-1257; SER-1281 AND SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[9]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY102267 mRNA. Translation: AAM52216.1.
AK041682 mRNA. Translation: BAC31031.1.
AK045159 mRNA. Translation: BAC32242.1.
AK048706 mRNA. Translation: BAC33427.1.
AK164853 mRNA. Translation: BAE37943.1.
BC096539 mRNA. Translation: AAH96539.1.
AK122414 mRNA. Translation: BAC65696.1.
CCDSCCDS39412.1. [Q4VA53-1]
RefSeqNP_780519.3. NM_175310.6. [Q4VA53-1]
XP_006504965.1. XM_006504902.1. [Q4VA53-3]
UniGeneMm.32959.

3D structure databases

ProteinModelPortalQ4VA53.
SMRQ4VA53. Positions 68-94, 349-418.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid221515. 2 interactions.
IntActQ4VA53. 1 interaction.
MINTMINT-5056218.

PTM databases

PhosphoSiteQ4VA53.

Proteomic databases

MaxQBQ4VA53.
PaxDbQ4VA53.
PRIDEQ4VA53.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000016569; ENSMUSP00000016569; ENSMUSG00000034021. [Q4VA53-1]
ENSMUST00000110486; ENSMUSP00000106112; ENSMUSG00000034021. [Q4VA53-2]
GeneID100710.
KEGGmmu:100710.
UCSCuc009auh.2. mouse. [Q4VA53-1]
uc009aui.1. mouse. [Q4VA53-2]
uc009auj.2. mouse. [Q4VA53-3]

Organism-specific databases

CTD23047.
MGIMGI:2140945. Pds5b.
RougeSearch...

Phylogenomic databases

eggNOGNOG268017.
GeneTreeENSGT00390000012488.
HOVERGENHBG108241.
InParanoidQ4VA53.
KOK11267.
OrthoDBEOG73Z2SD.
PhylomeDBQ4VA53.
TreeFamTF106415.

Gene expression databases

ArrayExpressQ4VA53.
BgeeQ4VA53.
GenevestigatorQ4VA53.

Family and domain databases

Gene3D1.25.10.10. 4 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR017956. AT_hook_DNA-bd_motif.
[Graphical view]
SMARTSM00384. AT_hook. 3 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 5 hits.
ProtoNetSearch...

Other

NextBio354602.
PROQ4VA53.
SOURCESearch...

Entry information

Entry namePDS5B_MOUSE
AccessionPrimary (citable) accession number: Q4VA53
Secondary accession number(s): Q3TNZ4 expand/collapse secondary AC list , Q7TSS4, Q80TM8, Q8BJ18, Q8BLH6, Q8BX77
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot