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Protein

Diacylglycerol O-acyltransferase 2

Gene

dgat2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation (By similarity).By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-DRE-1482883. Acyl chain remodeling of DAG and TAG.
R-DRE-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00282.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 2 (EC:2.3.1.20)
Alternative name(s):
Diglyceride acyltransferase 2
Gene namesi
Name:dgat2
ORF Names:zgc:113394
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 10

Organism-specific databases

ZFINiZDB-GENE-050913-15. dgat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242CytoplasmicSequence analysisAdd
BLAST
Transmembranei43 – 6119HelicalSequence analysisAdd
BLAST
Topological domaini62 – 654LumenalSequence analysis
Transmembranei66 – 8520HelicalSequence analysisAdd
BLAST
Topological domaini86 – 361276CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Diacylglycerol O-acyltransferase 2PRO_0000249048Add
BLAST

Proteomic databases

PaxDbiQ4V9F0.

Expressioni

Gene expression databases

BgeeiQ4V9F0.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000116381.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ4V9F0.
KOiK11160.
PhylomeDBiQ4V9F0.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V9F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTILAAYSG VKKGSGSSIL SALHDLPTVP WLTRSKMVKH LQVISVLQFI
60 70 80 90 100
MTFLTMGIAC SLLLMYMFCT DFWVISVLYV AWLIYDWNTP GQGGRRSTWV
110 120 130 140 150
RDWTVWKYMR DYFPIRLIKT HNLLPSRNYI FGYHPHGILC FGAFCNFGTE
160 170 180 190 200
ATGFTKVFPG IKPSLATLAG NFRLPMFREY LMCGGICPVN RNSIDYLLSS
210 220 230 240 250
NGTGNAVVIV IGGAAESLDC APGRNSVMLK KRKGFVKLAL KQGADLVPVY
260 270 280 290 300
SFGENEVYKQ LIFEEGSWWR TIQRKLQKFL GFAPCLFHGC GLFFPESWGL
310 320 330 340 350
VPYCKPITTV VGEPITVPKI EEPTQDVIDM YHAMYIRSLK SLFDNYKTRF
360
GLNESDTLII H
Length:361
Mass (Da):40,865
Last modified:July 5, 2005 - v1
Checksum:iE0B9A0E8E9D1A8B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096927 mRNA. Translation: AAH96927.1.
RefSeqiNP_001025367.1. NM_001030196.1.
UniGeneiDr.91373.

Genome annotation databases

EnsembliENSDART00000066793; ENSDARP00000066792; ENSDARG00000018846.
GeneIDi565316.
KEGGidre:565316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC096927 mRNA. Translation: AAH96927.1.
RefSeqiNP_001025367.1. NM_001030196.1.
UniGeneiDr.91373.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000116381.

Proteomic databases

PaxDbiQ4V9F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000066793; ENSDARP00000066792; ENSDARG00000018846.
GeneIDi565316.
KEGGidre:565316.

Organism-specific databases

CTDi84649.
ZFINiZDB-GENE-050913-15. dgat2.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ4V9F0.
KOiK11160.
PhylomeDBiQ4V9F0.

Enzyme and pathway databases

UniPathwayiUPA00282.
ReactomeiR-DRE-1482883. Acyl chain remodeling of DAG and TAG.
R-DRE-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ4V9F0.

Gene expression databases

BgeeiQ4V9F0.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiDGAT2_DANRE
AccessioniPrimary (citable) accession number: Q4V9F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2005
Last modified: June 8, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.