Q4V9F0 (DGAT2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol O-acyltransferase 2
EC=2.3.1.20

Alternative name(s):
Diglyceride acyltransferase 2

Gene names

Name:	dgat2
ORF Names:	zgc:113394

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Diacylglycerol O-acyltransferase 2		PRO_0000249048
Regions
	Topological domain	1 – 42	42	Cytoplasmic Potential
	Transmembrane	43 – 61	19	Helical; Potential
	Topological domain	62 – 65	4	Lumenal Potential
	Transmembrane	66 – 85	20	Helical; Potential
	Topological domain	86 – 361	276	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4V9F0 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: E0B9A0E8E9D1A8B0	FASTA	361	40,865
	10 20 30 40 50 60 MKTILAAYSG VKKGSGSSIL SALHDLPTVP WLTRSKMVKH LQVISVLQFI MTFLTMGIAC 70 80 90 100 110 120 SLLLMYMFCT DFWVISVLYV AWLIYDWNTP GQGGRRSTWV RDWTVWKYMR DYFPIRLIKT 130 140 150 160 170 180 HNLLPSRNYI FGYHPHGILC FGAFCNFGTE ATGFTKVFPG IKPSLATLAG NFRLPMFREY 190 200 210 220 230 240 LMCGGICPVN RNSIDYLLSS NGTGNAVVIV IGGAAESLDC APGRNSVMLK KRKGFVKLAL 250 260 270 280 290 300 KQGADLVPVY SFGENEVYKQ LIFEEGSWWR TIQRKLQKFL GFAPCLFHGC GLFFPESWGL 310 320 330 340 350 360 VPYCKPITTV VGEPITVPKI EEPTQDVIDM YHAMYIRSLK SLFDNYKTRF GLNESDTLII H

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC096927 mRNA. Translation: AAH96927.1.
IPI	IPI00495838.
RefSeq	NP_001025367.1. NM_001030196.1.
UniGene	Dr.91373.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4V9F0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000066793; ENSDARP00000066792; ENSDARG00000018846.
GeneID	565316.
KEGG	dre:565316.
NMPDR	fig|7955.3.peg.10591.
Organism-specific databases
CTD	84649.
ZFIN	ZDB-GENE-050913-15. dgat2.
Phylogenomic databases
eggNOG	fiNOG12802.
GeneTree	ENSGT00550000074276.
HOGENOM	HBG561030.
HOVERGEN	HBG065791.
InParanoid	Q4V9F0.
OMA	HKSTELE.
OrthoDB	EOG4R502S.
PhylomeDB	Q4V9F0.
Gene expression databases
Bgee	Q4V9F0.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
KO	K11160.
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGAT2_DANRE

Accession

Primary (citable) accession number: Q4V9F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program