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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

ube2s

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. small conjugating protein transferase activity Source: GO_Central
  4. ubiquitin protein ligase activity Source: GO_Central
  5. ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  1. activation of anaphase-promoting complex activity Source: UniProtKB
  2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  3. cell division Source: UniProtKB-KW
  4. exit from mitosis Source: UniProtKB
  5. free ubiquitin chain polymerization Source: UniProtKB
  6. protein K11-linked ubiquitination Source: UniProtKB
  7. protein polyubiquitination Source: GO_Central
  8. ubiquitin-dependent protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene namesi
Name:ube2s
ORF Names:zgc:114060
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 16

Organism-specific databases

ZFINiZDB-GENE-050913-92. ube2s.

Subcellular locationi

GO - Cellular componenti

  1. anaphase-promoting complex Source: UniProtKB
  2. cytoplasm Source: GO_Central
  3. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Ubiquitin-conjugating enzyme E2 SPRO_0000390429Add
BLAST

Expressioni

Gene expression databases

BgeeiQ4V908.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000047451.

Structurei

3D structure databases

ProteinModelPortaliQ4V908.
SMRiQ4V908. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ4V908.
KOiK10583.
OMAiGHKGEIC.
OrthoDBiEOG73JKXD.
PhylomeDBiQ4V908.
TreeFamiTF101120.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V908-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSNVENLPP QVLRLVYKEV SALAADPPEG IKIYPSEEDI TELHTSIEGP
60 70 80 90 100
EGTPYAGGVF RMRLVLGKDF PAVPPRGYFL TKIFHPNVGH KGEICVNVLK
110 120 130 140 150
RDWKAELGLR HVLLTIKCLL IHPNPESALN EEAGKLLLED YKEYASRAHL
160 170 180 190 200
LTEIHAMGGT SGAPQEPADG PQPKKHAGDP NKRVVGAGLP TMGTGTNNSN
210 220
ISNTNIVAKK KTDKKRALRR L
Length:221
Mass (Da):24,149
Last modified:July 5, 2005 - v1
Checksum:i3E3B6C7E6AC6B26D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097122 mRNA. Translation: AAH97122.1.
RefSeqiNP_001020707.1. NM_001025536.1.
UniGeneiDr.132389.

Genome annotation databases

EnsembliENSDART00000047452; ENSDARP00000047451; ENSDARG00000031775.
GeneIDi565498.
KEGGidre:565498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097122 mRNA. Translation: AAH97122.1.
RefSeqiNP_001020707.1. NM_001025536.1.
UniGeneiDr.132389.

3D structure databases

ProteinModelPortaliQ4V908.
SMRiQ4V908. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000047451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000047452; ENSDARP00000047451; ENSDARG00000031775.
GeneIDi565498.
KEGGidre:565498.

Organism-specific databases

CTDi27338.
ZFINiZDB-GENE-050913-92. ube2s.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ4V908.
KOiK10583.
OMAiGHKGEIC.
OrthoDBiEOG73JKXD.
PhylomeDBiQ4V908.
TreeFamiTF101120.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi20885915.
PROiQ4V908.

Gene expression databases

BgeeiQ4V908.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiUBE2S_DANRE
AccessioniPrimary (citable) accession number: Q4V908
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2005
Last modified: February 4, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.