Q4V908 (UBE2S_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S

Gene names

Name:	ube2s
ORF Names:	zgc:114060

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB free ubiquitin chain polymerization Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Ubiquitin-conjugating enzyme E2 S		PRO_0000390429
Sites
	Active site	95	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4V908 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 3E3B6C7E6AC6B26D	FASTA	221	24,149
	10 20 30 40 50 60 MNSNVENLPP QVLRLVYKEV SALAADPPEG IKIYPSEEDI TELHTSIEGP EGTPYAGGVF 70 80 90 100 110 120 RMRLVLGKDF PAVPPRGYFL TKIFHPNVGH KGEICVNVLK RDWKAELGLR HVLLTIKCLL 130 140 150 160 170 180 IHPNPESALN EEAGKLLLED YKEYASRAHL LTEIHAMGGT SGAPQEPADG PQPKKHAGDP 190 200 210 220 NKRVVGAGLP TMGTGTNNSN ISNTNIVAKK KTDKKRALRR L

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC097122 mRNA. Translation: AAH97122.1.
IPI	IPI00506623.
RefSeq	NP_001020707.1. NM_001025536.1.
UniGene	Dr.132389.
3D structure databases
ProteinModelPortal	Q4V908.
SMR	Q4V908. Positions 6-156.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000047451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000047452; ENSDARP00000047451; ENSDARG00000031775.
GeneID	565498.
KEGG	dre:565498.
Organism-specific databases
ZFIN	ZDB-GENE-050913-92. zgc:114060.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00680000099547.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
InParanoid	Q4V908.
KO	K10583.
OrthoDB	EOG4KPTBW.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q4V908.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20885915.

Entry information

Entry name

UBE2S_DANRE

Accession

Primary (citable) accession number: Q4V908

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	July 5, 2005
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program