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Protein

Calpain 11

Gene

Capn11

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.By similarity

Catalytic activityi

Broad endopeptidase specificity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Sequence analysis1
Active sitei272Sequence analysis1
Active sitei296Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi600 – 6111PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi630 – 6412PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain 11Imported (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 11By similarity
Short name:
CANP 11By similarity
Gene namesi
Name:Capn11Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1302946. Capn11.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003562201 – 716Calpain 11Add BLAST716

Proteomic databases

PaxDbiQ4V8Q1.
PRIDEiQ4V8Q1.

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) and a small (regulatory) subunit.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059934.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 354Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini587 – 620EF-hand 1PROSITE-ProRule annotationAdd BLAST34
Domaini617 – 652EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 528Domain IIIBy similarityAdd BLAST174
Regioni529 – 544LinkerBy similarityAdd BLAST16
Regioni545 – 715Domain IVBy similarityAdd BLAST171

Sequence similaritiesi

Belongs to the peptidase C2 family.Sequence analysis
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ4V8Q1.
KOiK08580.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q4V8Q1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLNFSDISYA ERGMVATINS NRLKDKGVGQ YQNAYNYKNQ NFEDLRASCL
60 70 80 90 100
KMGELFEDPF FPAEPQSIGF KELGPKSQEV HNVYWQRPKD IIHNPQFITN
110 120 130 140 150
DFSPTDICQG ILGDCWLLAA IGSLTTSPKL LFRVVPRSQS FKKNYAGIFH
160 170 180 190 200
FQLWQFGHWL NVVVDDRLPT KNNKLVFVHA SHRQEFWGAL LEKAYAKLNG
210 220 230 240 250
SYEALTGGST MEGLEDFTGG MAQCIRLQNP PHNLLRLLRK ALEKSSLMGC
260 270 280 290 300
SIEVTNSDDV EKMAHNMLVK GHAYAITGLQ DVYYRDKLET LIRIQNPWGR
310 320 330 340 350
IEWNGAWSDK AKEWEEVMPE VQMQLLHKKE DGEFWMSYED FMSNFTLLEI
360 370 380 390 400
CNLTPDALSS WDYKSCWHTT FFEGSWRRGS TAGGCRNYPE TFWSNPQFRI
410 420 430 440 450
SLPETDDPED SSDKSEMVCT CLVALMQKNW RHAREGPQLL TIGFVVFSVW
460 470 480 490 500
YVQFQNLQDI HLKKDFFMKY RDHGFSEIFT NTREVNSHLR LPPGEYVIIP
510 520 530 540 550
STFEPHKDAD FLLRVFTEKH SETWLLDEVN MLEQLQEETI TDADLDQNSV
560 570 580 590 600
ELFETLANRD SQVDMYDLQK LLNKMSSKVK SFKSKGFSLD VCRRMVNLMD
610 620 630 640 650
KDDSGKLGLH EFHILWKKIK KWMEIFKECD QDRSGNLNSY EMRLAIEKAG
660 670 680 690 700
IRMNNRVTEV VVARYADANM IVDFDNFINC FLRLKAMFAF FLSMDTKKTG
710
SICLNINQWL HITMWG
Length:716
Mass (Da):83,241
Last modified:December 16, 2008 - v2
Checksum:i634800A30B3CA3D4
GO
Isoform 21 Publication (identifier: Q4V8Q1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:703
Mass (Da):81,756
Checksum:i9549EF5D7B0E4F16
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti254V → A in AAH97256 (PubMed:15489334).Curated1
Sequence conflicti450 – 453WYVQ → PKE in AAH97256 (PubMed:15489334).Curated4
Sequence conflicti579V → F in AAH97256 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0529931 – 13Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC097557 Genomic DNA. No translation available.
BC097256 mRNA. Translation: AAH97256.1.
BN000321 mRNA. Translation: CAE48376.1.
RefSeqiNP_001002806.2. NM_001002806.2.
UniGeneiRn.123410.

Genome annotation databases

GeneIDi408218.
KEGGirno:408218.
UCSCiRGD:1302946. rat. [Q4V8Q1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC097557 Genomic DNA. No translation available.
BC097256 mRNA. Translation: AAH97256.1.
BN000321 mRNA. Translation: CAE48376.1.
RefSeqiNP_001002806.2. NM_001002806.2.
UniGeneiRn.123410.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000059934.

Protein family/group databases

MEROPSiC02.013.

Proteomic databases

PaxDbiQ4V8Q1.
PRIDEiQ4V8Q1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi408218.
KEGGirno:408218.
UCSCiRGD:1302946. rat. [Q4V8Q1-1]

Organism-specific databases

CTDi11131.
RGDi1302946. Capn11.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ4V8Q1.
KOiK08580.

Miscellaneous databases

PROiQ4V8Q1.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN11_RAT
AccessioniPrimary (citable) accession number: Q4V8Q1
Secondary accession number(s): Q6IE72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: November 30, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.