Q4V8J2 (UB2L6_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin/ISG15-conjugating enzyme E2 L6
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein L6
Ubiquitin-protein ligase L6

Gene names

Name:

Ube2l6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3 By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RNF19A, RNF19B and RNF144B. Interacts with FLT3 (tyrosine phosphorylated) By similarity.
Post-translational modification	ISGylated By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ISG15-protein conjugation Inferred from electronic annotation. Source: Compara modification-dependent protein catabolic process Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ISG15 ligase activity Inferred from electronic annotation. Source: Compara ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 153	153	Ubiquitin/ISG15-conjugating enzyme E2 L6		PRO_0000375221
Sites
	Active site	86	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4V8J2 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 47E1AC421ADC756E	FASTA	153	17,807
	10 20 30 40 50 60 MTASKRVAKE LDDLSKELPP YLRHLSSDDA NVLVWHMLLL PDQLPYRLKA FGLRIDFPRE 70 80 90 100 110 120 YPLKPPTLRF TTKIYHPNIS EDGLVCLPLI STENWKPYTK AYQVLEALNI LVSRPNLEEP 130 140 150 VRLELADLLT QDPEMFRKKA EEFTLQYGVD RPS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC097364 mRNA. Translation: AAH97364.1.
IPI	IPI00372788.
RefSeq	NP_001019926.1. NM_001024755.1.
UniGene	Rn.8609.
3D structure databases
HSSP	HSSP built from PDB template 1WZV based on UniProtKB O14933.
ProteinModelPortal	Q4V8J2.
SMR	Q4V8J2. Positions 3-152.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000039001.
Proteomic databases
PRIDE	Q4V8J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000044319; ENSRNOP00000039001; ENSRNOG00000030467.
GeneID	295704.
KEGG	rno:295704.
UCSC	RGD:1307960. rat.
Organism-specific databases
CTD	9246.
RGD	1307960. Ube2l6.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00700000104033.
HOGENOM	HOG000233455.
InParanoid	Q4V8J2.
KO	K04553.
OMA	PEKPPYN.
OrthoDB	EOG43BMQ6.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q4V8J2.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	639925.

Entry information

Entry name

UB2L6_RAT

Accession

Primary (citable) accession number: Q4V8J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	July 5, 2005
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program