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Protein

Volume-regulated anion channel subunit LRRC8A

Gene

Lrrc8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Required for channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition. Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (By similarity). Also required for T-cell development (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Differentiation, Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8A
Alternative name(s):
Leucine-rich repeat-containing protein 8A
Gene namesi
Name:Lrrc8a
Synonyms:Lrrc8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1311690. Lrrc8a.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: The leucine-rich repeat (LRR) domain is on the cytoplasmic side of the cell membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Topological domaini44 – 12380ExtracellularSequence analysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence analysisAdd
BLAST
Topological domaini145 – 268124CytoplasmicSequence analysisAdd
BLAST
Transmembranei269 – 28921HelicalSequence analysisAdd
BLAST
Topological domaini290 – 32334ExtracellularSequence analysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence analysisAdd
BLAST
Topological domaini345 – 810466CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 810810Volume-regulated anion channel subunit LRRC8APRO_0000084501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence analysis
Modified residuei200 – 2001PhosphothreonineBy similarity
Modified residuei202 – 2021PhosphoserineCombined sources
Modified residuei217 – 2171PhosphoserineCombined sources

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ4V8I7.
PRIDEiQ4V8I7.

PTM databases

iPTMnetiQ4V8I7.
PhosphoSiteiQ4V8I7.

Expressioni

Gene expression databases

GenevisibleiQ4V8I7. RN.

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8B, LRRC8C, LRRC8D and/or LRRC8E), possibly to form a heterohexamer. In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031726.

Structurei

3D structure databases

ProteinModelPortaliQ4V8I7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati373 – 40129LRR 1Add
BLAST
Repeati402 – 42423LRR 2Add
BLAST
Repeati425 – 44723LRR 3Add
BLAST
Repeati448 – 47023LRR 4Add
BLAST
Repeati471 – 49424LRR 5Add
BLAST
Repeati495 – 51824LRR 6Add
BLAST
Repeati519 – 54527LRR 7Add
BLAST
Repeati546 – 56823LRR 8Add
BLAST
Repeati569 – 59224LRR 9Add
BLAST
Repeati593 – 61523LRR 10Add
BLAST
Repeati616 – 64025LRR 11Add
BLAST
Repeati641 – 66323LRR 12Add
BLAST
Repeati664 – 68623LRR 13Add
BLAST
Repeati687 – 70620LRR 14Add
BLAST
Repeati707 – 73226LRR 15Add
BLAST
Repeati733 – 75523LRR 16Add
BLAST
Repeati756 – 77722LRR 17Add
BLAST

Sequence similaritiesi

Belongs to the LRRC8 family.Curated
Contains 17 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ4V8I7.
OMAiHIAYIPM.
OrthoDBiEOG7Z3F3T.
PhylomeDBiQ4V8I7.
TreeFamiTF331443.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin_like.
[Graphical view]
PfamiPF13855. LRR_8. 3 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V8I7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVTELRYF ADTQPAYRIL KPWWDVFTDY ISIVMLMIAV FGGTLQVTQD
60 70 80 90 100
KMICLPCKWV TKDSCNDSFR GWGASSPEPT YPNSTVLPTP DTGPTGIKYD
110 120 130 140 150
LDRHQYNYVD AVCYENRLHW FAKYFPYLVL LHTLIFLACS NFWFKFPRTS
160 170 180 190 200
SKLEHFVSIL LKCFDSPWTT RALSETVVEE SDPKPAFSKM NGSMDKKSST
210 220 230 240 250
VSEDVEATVP MLQRTKSRIE QGIVDRSETG VLDKKEGEQA KALFEKVKKF
260 270 280 290 300
RTHVEEGDIV YRLYMRQTII KVIKFFLIIC YTVYYVHNIK FDVDCTVDIE
310 320 330 340 350
SLTGYRTYRC AHPLATLFKI LASFYISLVI FYGLICMYTL WWMLRRSLKK
360 370 380 390 400
YSFESIREES SYSDIPDVKN DFAFMLHLID QYDPLYSKRF AVFLSEVSEN
410 420 430 440 450
KLRQLNLNNE WTLDKLRQRL TKNAQDKLEL HLFMLSGIPD TVFDLLELEV
460 470 480 490 500
LKLELIPDVT IPPSIAQLTG LKELWLYHTA AKIEAPALAF LRENLRALHI
510 520 530 540 550
KFTDIKEIPL WIYSLKTLEE LHLTGNLSAE NNRYIVIDGL RELKRLKVLR
560 570 580 590 600
LKSNLSKLPQ VVTDVGVHLQ KLSINNEGTK LIVLNSLKKM VNLTELELIR
610 620 630 640 650
CDLERIPHSI FSLHNLQEID LKDNNLKTIE EIISFQHLHR LTCLKLWYNH
660 670 680 690 700
IAYIPIQIGN LTNLERLYLN RNKIEKIPTQ LFYCRKLRYL DLSHNNLTLL
710 720 730 740 750
PADIGLLQNL QNLAVTANRI EALPPELFQC RKLRALHLGN NVLQSLPSRV
760 770 780 790 800
GELTNLTQIE LRGNRLECLP VELGECPLLK RSGLVVEEDL FSTLPPEVKE
810
RLWRADKEQA
Length:810
Mass (Da):94,162
Last modified:July 5, 2005 - v1
Checksum:iA05EE234C10615C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097371 mRNA. Translation: AAH97371.1.
RefSeqiNP_001019953.1. NM_001024782.1.
XP_006233876.1. XM_006233814.2.
XP_008759882.1. XM_008761660.1.
UniGeneiRn.33512.

Genome annotation databases

EnsembliENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296.
ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296.
GeneIDi311846.
KEGGirno:311846.
UCSCiRGD:1311690. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097371 mRNA. Translation: AAH97371.1.
RefSeqiNP_001019953.1. NM_001024782.1.
XP_006233876.1. XM_006233814.2.
XP_008759882.1. XM_008761660.1.
UniGeneiRn.33512.

3D structure databases

ProteinModelPortaliQ4V8I7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031726.

PTM databases

iPTMnetiQ4V8I7.
PhosphoSiteiQ4V8I7.

Proteomic databases

PaxDbiQ4V8I7.
PRIDEiQ4V8I7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296.
ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296.
GeneIDi311846.
KEGGirno:311846.
UCSCiRGD:1311690. rat.

Organism-specific databases

CTDi56262.
RGDi1311690. Lrrc8a.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ4V8I7.
OMAiHIAYIPM.
OrthoDBiEOG7Z3F3T.
PhylomeDBiQ4V8I7.
TreeFamiTF331443.

Miscellaneous databases

NextBioi664293.
PROiQ4V8I7.

Gene expression databases

GenevisibleiQ4V8I7. RN.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin_like.
[Graphical view]
PfamiPF13855. LRR_8. 3 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202 AND SER-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLRC8A_RAT
AccessioniPrimary (citable) accession number: Q4V8I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 5, 2005
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.