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Protein

Centromere protein U

Gene

Cenpu

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein U
Short name:
CENP-U
Alternative name(s):
MLF1-interacting protein
Gene namesi
Name:Cenpu
Synonyms:Mlf1ip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1562566. Cenpu.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Centromere protein UPRO_0000247674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731Phosphothreonine; by PLK1By similarity
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei110 – 1101PhosphoserineBy similarity
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei130 – 1301PhosphoserineBy similarity
Modified residuei133 – 1331PhosphoserineBy similarity
Modified residuei135 – 1351PhosphoserineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei224 – 2241PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PLK1 at Thr-73, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4V8G7.
PRIDEiQ4V8G7.

PTM databases

iPTMnetiQ4V8G7.
PhosphoSiteiQ4V8G7.

Expressioni

Tissue specificityi

Expressed at high levels in glioblastoma cell lines. Up-regulated in GBM (glioblastoma multiforme) tumors. Significantly increased in both the tumor core as well as the contralateral striatum and cortex in gliomas.1 Publication

Gene expression databases

BgeeiENSRNOG00000022261.
GenevisibleiQ4V8G7. RN.

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with MLF1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046417.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili289 – 35264Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi4 – 2118Nuclear localization signalSequence analysisAdd
BLAST
Motifi295 – 31218Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi56 – 616Poly-Glu

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J00R. Eukaryota.
ENOG410Z48V. LUCA.
GeneTreeiENSGT00390000015511.
HOGENOMiHOG000236255.
HOVERGENiHBG081090.
InParanoidiQ4V8G7.
KOiK11513.
OMAiKQLHQDY.
OrthoDBiEOG091G09Q1.
PhylomeDBiQ4V8G7.
TreeFamiTF330780.

Family and domain databases

InterProiIPR025214. CENP-U.
[Graphical view]
PfamiPF13097. CENP-U. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V8G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARRSLRYS GDPGAKRSRN TLGSTNSRKQ KAGQKPKRKD VFDFPNTSDV
60 70 80 90 100
SSMLRELEEE EPYETFDPPL HSTAIYTDDE LYKHCVSSTS PATHRGKESR
110 120 130 140 150
NLNPSENEAS GNDSIKLSAK KPRRKLEPIS DESDSSEDNV RRSVSIERPR
160 170 180 190 200
ARKPPAAPAA ASSSSSPSER PAEQVTPRKT SFPQPSAVEE TPAAQSQLKT
210 220 230 240 250
QKKVRPSPGR RKRPRRGSTH SDASESMHIL CLEGKRQSDV MELDVVLSAF
260 270 280 290 300
ERTFLDYKQR VESESCNQAI SKFYFKIKGE LIRMLKEVQM LKALKRKNTK
310 320 330 340 350
IISNMEKKRQ RLIDVQDELI RLEPQLKQLQ TKYDDLKKRK SALKNSKHFL
360 370 380 390 400
SNLKQLYQDY SNVREEEPKE KEKYDSSSLP ALLFKARSIL GAEKHLKTIN
410
YHLGKLLKQD
Length:410
Mass (Da):46,801
Last modified:July 5, 2005 - v1
Checksum:i0B6EB7DE22C2100A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097399 mRNA. Translation: AAH97399.1.
RefSeqiNP_001020844.1. NM_001025673.1.
UniGeneiRn.128609.

Genome annotation databases

EnsembliENSRNOT00000041596; ENSRNOP00000046417; ENSRNOG00000022261.
GeneIDi306464.
KEGGirno:306464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097399 mRNA. Translation: AAH97399.1.
RefSeqiNP_001020844.1. NM_001025673.1.
UniGeneiRn.128609.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046417.

PTM databases

iPTMnetiQ4V8G7.
PhosphoSiteiQ4V8G7.

Proteomic databases

PaxDbiQ4V8G7.
PRIDEiQ4V8G7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041596; ENSRNOP00000046417; ENSRNOG00000022261.
GeneIDi306464.
KEGGirno:306464.

Organism-specific databases

CTDi79682.
RGDi1562566. Cenpu.

Phylogenomic databases

eggNOGiENOG410J00R. Eukaryota.
ENOG410Z48V. LUCA.
GeneTreeiENSGT00390000015511.
HOGENOMiHOG000236255.
HOVERGENiHBG081090.
InParanoidiQ4V8G7.
KOiK11513.
OMAiKQLHQDY.
OrthoDBiEOG091G09Q1.
PhylomeDBiQ4V8G7.
TreeFamiTF330780.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ4V8G7.

Gene expression databases

BgeeiENSRNOG00000022261.
GenevisibleiQ4V8G7. RN.

Family and domain databases

InterProiIPR025214. CENP-U.
[Graphical view]
PfamiPF13097. CENP-U. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCENPU_RAT
AccessioniPrimary (citable) accession number: Q4V8G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.