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Protein

Transcription factor IIIB 50 kDa subunit

Gene

Brf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7ZincBy similarity1
Metal bindingi10ZincBy similarity1
Metal bindingi28ZincBy similarity1
Metal bindingi31ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:Brf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi1308817 Brf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003371901 – 416Transcription factor IIIB 50 kDa subunitAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350PhosphoserineBy similarity1
Modified residuei358Cysteine sulfenic acid (-SOH)By similarity1

Post-translational modificationi

In response to oxidative stress, Cys-358 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-358 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.By similarity

Keywords - PTMi

Oxidation, Phosphoprotein

Proteomic databases

PaxDbiQ4V8D6
PRIDEiQ4V8D6

Expressioni

Gene expression databases

BgeeiENSRNOG00000012739
GenevisibleiQ4V8D6 RN

Interactioni

Subunit structurei

Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017324

Structurei

3D structure databases

ProteinModelPortaliQ4V8D6
SMRiQ4V8D6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 1571Add BLAST86
Repeati173 – 2492Add BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 114Interaction with target DNABy similarity7
Regioni354 – 360Required for the formation of a ternary complex with DNA and TBP; not required for interaction with TBP in the absence of DNABy similarity7
Regioni362 – 416Required for interaction with TBP and formation of a ternary complex with DNA and TBPBy similarityAdd BLAST55

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1598 Eukaryota
COG1405 LUCA
GeneTreeiENSGT00390000002288
HOGENOMiHOG000095260
HOVERGENiHBG107537
InParanoidiQ4V8D6
KOiK15197
OMAiCMLKPPK
OrthoDBiEOG091G0ADG
PhylomeDBiQ4V8D6
TreeFamiTF331596

Family and domain databases

InterProiView protein in InterPro
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PfamiView protein in Pfam
PF08271 TF_Zn_Ribbon, 1 hit
SUPFAMiSSF47954 SSF47954, 1 hit
PROSITEiView protein in PROSITE
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

Q4V8D6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNGSRCPDC GSSELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL
60 70 80 90 100
REVTYSRSTG ENEQVSRSQQ RDLRRVRDLC RILKLPLTFE ETAVSYYQKA
110 120 130 140 150
YQLSGIRAAR LQKKEVVVGC CVLITCRQHN WPLTMGAICT LLYADLDVFS
160 170 180 190 200
STYMQIVKLL GLDVPSLCLA DLVKSYCSSF KLFQASPSMP AKYVEDKDKM
210 220 230 240 250
LSRTLLLVEL ANETWLVTGR HPLPIITAAT FLAWQSLRPS DRLTCSLARF
260 270 280 290 300
CKLANVDLPY PAASRLQELL AVLLQMASQL AWLQVLRLDK RSVVKHIGDL
310 320 330 340 350
LQHRHMLVRM AFQDGTAEVE TKQQQPQGRG QQEEVGDSTF DLPKRKRPAS
360 370 380 390 400
PALLLPPCML KPPKRTHTMP PDSVVTGDED ISDSEIEQYL RTPQEVRDFE
410
RAQAASRAAM SVPNPP
Length:416
Mass (Da):46,596
Last modified:July 5, 2005 - v1
Checksum:i73A78D97DCD85CB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097435 mRNA Translation: AAH97435.1
RefSeqiNP_001019944.1, NM_001024773.1
UniGeneiRn.163328

Genome annotation databases

EnsembliENSRNOT00000017324; ENSRNOP00000017324; ENSRNOG00000012739
GeneIDi306542
KEGGirno:306542
UCSCiRGD:1308817 rat

Similar proteinsi

Entry informationi

Entry nameiBRF2_RAT
AccessioniPrimary (citable) accession number: Q4V8D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 5, 2005
Last modified: June 20, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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