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Protein

Cytochrome P450 2U1

Gene

Cyp2u1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes (By similarity).By similarity

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi476 – 4761Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: GO_Central
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  5. oxygen binding Source: GO_Central
  6. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. exogenous drug catabolic process Source: GO_Central
  2. organic acid metabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_346758. Miscellaneous substrates.
REACT_351012. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2U1 (EC:1.14.14.1)
Gene namesi
Name:Cyp2u1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1309433. Cyp2u1.

Subcellular locationi

  1. Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
  2. Microsome membrane 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Transmembranei247 – 26721HelicalSequence AnalysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence AnalysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Cytochrome P450 2U1PRO_0000291758Add
BLAST

Expressioni

Tissue specificityi

Specifically expressed in thymus and brain. In brain, expressed in cortex, cerebellum, olfactory bulbs, pons and medulla and the limbic structures (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ4V8D1.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052433.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ4V8D1.
KOiK07422.
OMAiIEEFAYV.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ4V8D1.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V8D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIGGLRPA AGEQPGVGPH LQAVGGALLL CGLAVLLDWV WLQRQRAGGI
60 70 80 90 100
PPGPKPRPLV GNFGYLLLPR FLRLHFWLGS GSQTDTVGRH VYLARLARVY
110 120 130 140 150
GNIFSFFIGH RLVVVLSDFQ SVREALVQQA EVFSDRPRMP LISILTKEKG
160 170 180 190 200
IVFAHYGPIW KQQRRFSHST LRHFGLGKLS LEPRIIEEFA YVKAEMQKHG
210 220 230 240 250
EAPFSPFPVI SNAVSNIICS LCFGQRFDYT NKEFKKVLDF MSRGLEICLH
260 270 280 290 300
SQLFLINLCP WFYYLPFGPF KELRQIERDI TCFLKNIIKE HQESLDANNP
310 320 330 340 350
QDFIDMYLLH TQEEKDKCKG TNFDEDYLFY IIGDLFIAGT DTTTNSLLWC
360 370 380 390 400
LLYMSLNPGV QKKVHEEIER VIGRDRAPSL TDKAQMPYTE ATIMEVQRLS
410 420 430 440 450
MVVPLAIPHM TSEKTVLQGY SIPKGTVVLP NLWSIHRDPV IWEKPDDFCP
460 470 480 490 500
HRFLDDQGQL LKRETFIPFG IGKRVCMGEQ LAKMELFLMF VSLMQSFTFA
510 520 530
LPEGSEKPIM TGRFGLTLAP HPFNVTVSKR
Length:530
Mass (Da):60,399
Last modified:July 5, 2005 - v1
Checksum:i7BDF8BD34265801A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097442 mRNA. Translation: AAH97442.1.
RefSeqiNP_001019950.1. NM_001024779.1.
XP_006233372.1. XM_006233310.2.
UniGeneiRn.161862.

Genome annotation databases

EnsembliENSRNOT00000055570; ENSRNOP00000052433; ENSRNOG00000011053.
GeneIDi310848.
KEGGirno:310848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097442 mRNA. Translation: AAH97442.1.
RefSeqiNP_001019950.1. NM_001024779.1.
XP_006233372.1. XM_006233310.2.
UniGeneiRn.161862.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000055570; ENSRNOP00000052433; ENSRNOG00000011053.
GeneIDi310848.
KEGGirno:310848.

Organism-specific databases

CTDi113612.
RGDi1309433. Cyp2u1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ4V8D1.
KOiK07422.
OMAiIEEFAYV.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ4V8D1.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_346758. Miscellaneous substrates.
REACT_351012. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

NextBioi662741.
PROiQ4V8D1.

Gene expression databases

GenevestigatoriQ4V8D1.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Characterization and tissue distribution of a novel human cytochrome P450-CYP2U1."
    Karlgren M., Backlund M., Johansson I., Oscarson M., Ingelman-Sundberg M.
    Biochem. Biophys. Res. Commun. 315:679-685(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCP2U1_RAT
AccessioniPrimary (citable) accession number: Q4V8D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2005
Last modified: April 1, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.