Q4V8C7 (PRKRA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon-inducible double stranded RNA-dependent protein kinase activator A

Alternative name(s):
Protein activator of the interferon-induced protein kinase
Protein kinase, interferon-inducible double stranded RNA-dependent activator

Gene names

Name:

Prkra

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 By similarity.
Subunit structure	Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, EIF2C2/AGO2 and TARBP2. Also able to interact with dsRNA By similarity.
Subcellular location	Cytoplasm › perinuclear region By similarity.
Domain	Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3 By similarity.
Post-translational modification	Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress By similarity.
Sequence similarities	Belongs to the PRKRA family. Contains 3 DRBM (double-stranded RNA-binding) domains.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing
Cellular component	Cytoplasm
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	production of siRNA involved in RNA interference Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Interferon-inducible double stranded RNA-dependent protein kinase activator A		PRO_0000223611
Regions
	Domain	34 – 101	68	DRBM 1
	Domain	126 – 194	69	DRBM 2
	Domain	240 – 308	69	DRBM 3
	Region	1 – 103	103	Sufficient for self-association and interaction with TARBP2 By similarity
	Region	102 – 195	94	Sufficient for self-association and interaction with TARBP2 By similarity
	Region	195 – 313	119	Sufficient for self-association and interaction with TARBP2 By similarity
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.2
	Modified residue	246	1	Phosphoserine By similarity
	Modified residue	287	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4V8C7 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 78550978D5B92043	FASTA	313	34,355
	10 20 30 40 50 60 MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGTKTKNIP VYECERSDVQ 70 80 90 100 110 120 VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ 130 140 150 160 170 180 PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA 190 200 210 220 230 240 KRNAAEKFLA KFSNISPENH ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD 250 260 270 280 290 300 YIQLLSEIAK EQGFSITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH 310 NALQYLKIIA ERK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS." Moser K., White F.M. J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY. Strain: Fischer. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC097446 mRNA. Translation: AAH97446.1.
IPI	IPI00187767.
RefSeq	NP_001019951.1. NM_001024780.1.
UniGene	Rn.3217.
3D structure databases
ProteinModelPortal	Q4V8C7.
SMR	Q4V8C7. Positions 28-104, 127-192.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000015624.
PTM databases
PhosphoSite	Q4V8C7.
Proteomic databases
PaxDb	Q4V8C7.
PRIDE	Q4V8C7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	311130.
KEGG	rno:311130.
UCSC	RGD:1306707. rat.
Organism-specific databases
CTD	8575.
RGD	1306707. Prkra.
Phylogenomic databases
eggNOG	NOG245126.
HOGENOM	HOG000231919.
HOVERGEN	HBG001700.
InParanoid	Q4V8C7.
OrthoDB	EOG41RPVB.
Gene expression databases
Genevestigator	Q4V8C7.
GermOnline	ENSRNOG00000011195. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.160.20. 3 hits.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. [Graphical view]
Pfam	PF00035. dsrm. 2 hits. [Graphical view]
SMART	SM00358. DSRM. 3 hits. [Graphical view]
PROSITE	PS50137. DS_RBD. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	663036.

Entry information

Entry name

PRKRA_RAT

Accession

Primary (citable) accession number: Q4V8C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	July 5, 2005
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program