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Q4V8C7

- PRKRA_RAT

UniProt

Q4V8C7 - PRKRA_RAT

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Protein

Interferon-inducible double-stranded RNA-dependent protein kinase activator A

Gene

Prkra

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association, sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).By similarity

GO - Molecular functioni

  1. protein kinase binding Source: RGD
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of cell proliferation Source: RGD
  2. production of siRNA involved in RNA interference Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
Alternative name(s):
Protein activator of the interferon-induced protein kinase
Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Gene namesi
Name:Prkra
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1306707. Prkra.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Interferon-inducible double-stranded RNA-dependent protein kinase activator APRO_0000223611Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei246 – 2461PhosphoserineBy similarity
Modified residuei287 – 2871PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4V8C7.
PRIDEiQ4V8C7.

PTM databases

PhosphoSiteiQ4V8C7.

Expressioni

Gene expression databases

GenevestigatoriQ4V8C7.

Interactioni

Subunit structurei

Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA (By similarity). Interacts with UBC9 (By similarity). Forms a complex with UBC9 and p53/TP53 (By similarity). Interacts with DUS2L (via DRBM domain) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015624.

Structurei

3D structure databases

ProteinModelPortaliQ4V8C7.
SMRiQ4V8C7. Positions 28-104, 127-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 10168DRBM 1PROSITE-ProRule annotationAdd
BLAST
Domaini126 – 19469DRBM 2PROSITE-ProRule annotationAdd
BLAST
Domaini240 – 30869DRBM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 103103Sufficient for self-association and interaction with TARBP2By similarityAdd
BLAST
Regioni102 – 19594Sufficient for self-association and interaction with TARBP2By similarityAdd
BLAST
Regioni195 – 313119Sufficient for self-association and interaction with TARBP2By similarityAdd
BLAST

Domaini

Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.By similarity

Sequence similaritiesi

Belongs to the PRKRA family.Curated
Contains 3 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG245126.
HOGENOMiHOG000231919.
HOVERGENiHBG001700.
InParanoidiQ4V8C7.
PhylomeDBiQ4V8C7.

Family and domain databases

Gene3Di3.30.160.20. 3 hits.
InterProiIPR014720. dsRNA-bd_dom.
[Graphical view]
PfamiPF00035. dsrm. 2 hits.
[Graphical view]
SMARTiSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEiPS50137. DS_RBD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V8C7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGTKTKNIP
60 70 80 90 100
VYECERSDVQ VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK
110 120 130 140 150
ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE
160 170 180 190 200
GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH
210 220 230 240 250
ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD YIQLLSEIAK
260 270 280 290 300
EQGFSITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH
310
NALQYLKIIA ERK
Length:313
Mass (Da):34,355
Last modified:July 5, 2005 - v1
Checksum:i78550978D5B92043
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097446 mRNA. Translation: AAH97446.1.
RefSeqiNP_001019951.1. NM_001024780.1.
UniGeneiRn.3217.

Genome annotation databases

GeneIDi311130.
KEGGirno:311130.
UCSCiRGD:1306707. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097446 mRNA. Translation: AAH97446.1 .
RefSeqi NP_001019951.1. NM_001024780.1.
UniGenei Rn.3217.

3D structure databases

ProteinModelPortali Q4V8C7.
SMRi Q4V8C7. Positions 28-104, 127-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015624.

PTM databases

PhosphoSitei Q4V8C7.

Proteomic databases

PaxDbi Q4V8C7.
PRIDEi Q4V8C7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 311130.
KEGGi rno:311130.
UCSCi RGD:1306707. rat.

Organism-specific databases

CTDi 8575.
RGDi 1306707. Prkra.

Phylogenomic databases

eggNOGi NOG245126.
HOGENOMi HOG000231919.
HOVERGENi HBG001700.
InParanoidi Q4V8C7.
PhylomeDBi Q4V8C7.

Miscellaneous databases

NextBioi 663036.
PROi Q4V8C7.

Gene expression databases

Genevestigatori Q4V8C7.

Family and domain databases

Gene3Di 3.30.160.20. 3 hits.
InterProi IPR014720. dsRNA-bd_dom.
[Graphical view ]
Pfami PF00035. dsrm. 2 hits.
[Graphical view ]
SMARTi SM00358. DSRM. 3 hits.
[Graphical view ]
PROSITEi PS50137. DS_RBD. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
    Moser K., White F.M.
    J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRKRA_RAT
AccessioniPrimary (citable) accession number: Q4V8C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3