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Q4V8C7 (PRKRA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
Alternative name(s):
Protein activator of the interferon-induced protein kinase
Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Gene names
Name:Prkra
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association, sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner By similarity.

Subunit structure

Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA By similarity. Interacts with UBC9 By similarity. Forms a complex with UBC9 and p53/TP53 By similarity. Interacts with DUS2L (via DRBM domain) By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity. Cytoplasm By similarity.

Domain

Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3 By similarity.

Post-translational modification

Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress By similarity.

Sequence similarities

Belongs to the PRKRA family.

Contains 3 DRBM (double-stranded RNA-binding) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Interferon-inducible double-stranded RNA-dependent protein kinase activator A
PRO_0000223611

Regions

Domain34 – 10168DRBM 1
Domain126 – 19469DRBM 2
Domain240 – 30869DRBM 3
Region1 – 103103Sufficient for self-association and interaction with TARBP2 By similarity
Region102 – 19594Sufficient for self-association and interaction with TARBP2 By similarity
Region195 – 313119Sufficient for self-association and interaction with TARBP2 By similarity

Amino acid modifications

Modified residue181Phosphoserine By similarity
Modified residue2461Phosphoserine By similarity
Modified residue2871Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4V8C7 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 78550978D5B92043

FASTA31334,355
        10         20         30         40         50         60 
MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGTKTKNIP VYECERSDVQ 

        70         80         90        100        110        120 
VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ 

       130        140        150        160        170        180 
PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA 

       190        200        210        220        230        240 
KRNAAEKFLA KFSNISPENH ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD 

       250        260        270        280        290        300 
YIQLLSEIAK EQGFSITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH 

       310 
NALQYLKIIA ERK 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[2]"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
Moser K., White F.M.
J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC097446 mRNA. Translation: AAH97446.1.
RefSeqNP_001019951.1. NM_001024780.1.
UniGeneRn.3217.

3D structure databases

ProteinModelPortalQ4V8C7.
SMRQ4V8C7. Positions 28-104, 127-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000015624.

PTM databases

PhosphoSiteQ4V8C7.

Proteomic databases

PaxDbQ4V8C7.
PRIDEQ4V8C7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID311130.
KEGGrno:311130.
UCSCRGD:1306707. rat.

Organism-specific databases

CTD8575.
RGD1306707. Prkra.

Phylogenomic databases

eggNOGNOG245126.
HOGENOMHOG000231919.
HOVERGENHBG001700.
InParanoidQ4V8C7.
PhylomeDBQ4V8C7.

Gene expression databases

GenevestigatorQ4V8C7.

Family and domain databases

Gene3D3.30.160.20. 3 hits.
InterProIPR014720. dsRNA-bd_dom.
[Graphical view]
PfamPF00035. dsrm. 2 hits.
[Graphical view]
SMARTSM00358. DSRM. 3 hits.
[Graphical view]
PROSITEPS50137. DS_RBD. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio663036.
PROQ4V8C7.

Entry information

Entry namePRKRA_RAT
AccessionPrimary (citable) accession number: Q4V8C7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families