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Q4V8C7

- PRKRA_RAT

UniProt

Q4V8C7 - PRKRA_RAT

Protein

Interferon-inducible double-stranded RNA-dependent protein kinase activator A

Gene

Prkra

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association, sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner By similarity.By similarity

    GO - Molecular functioni

    1. protein kinase binding Source: RGD
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. positive regulation of cell proliferation Source: RGD
    2. production of siRNA involved in RNA interference Source: UniProtKB

    Keywords - Biological processi

    RNA-mediated gene silencing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon-inducible double-stranded RNA-dependent protein kinase activator A
    Alternative name(s):
    Protein activator of the interferon-induced protein kinase
    Protein kinase, interferon-inducible double-stranded RNA-dependent activator
    Gene namesi
    Name:Prkra
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1306707. Prkra.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Interferon-inducible double-stranded RNA-dependent protein kinase activator APRO_0000223611Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei18 – 181PhosphoserineBy similarity
    Modified residuei246 – 2461PhosphoserineBy similarity
    Modified residuei287 – 2871PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ4V8C7.
    PRIDEiQ4V8C7.

    PTM databases

    PhosphoSiteiQ4V8C7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ4V8C7.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with EIF2AK2/PKR through its DRBM domains. Interacts with DICER1, AGO2 and TARBP2. Also able to interact with dsRNA By similarity. Interacts with UBC9 By similarity. Forms a complex with UBC9 and p53/TP53 By similarity. Interacts with DUS2L (via DRBM domain) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015624.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4V8C7.
    SMRiQ4V8C7. Positions 28-104, 127-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 10168DRBM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini126 – 19469DRBM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini240 – 30869DRBM 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 103103Sufficient for self-association and interaction with TARBP2By similarityAdd
    BLAST
    Regioni102 – 19594Sufficient for self-association and interaction with TARBP2By similarityAdd
    BLAST
    Regioni195 – 313119Sufficient for self-association and interaction with TARBP2By similarityAdd
    BLAST

    Domaini

    Self-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.By similarity

    Sequence similaritiesi

    Belongs to the PRKRA family.Curated
    Contains 3 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG245126.
    HOGENOMiHOG000231919.
    HOVERGENiHBG001700.
    InParanoidiQ4V8C7.
    PhylomeDBiQ4V8C7.

    Family and domain databases

    Gene3Di3.30.160.20. 3 hits.
    InterProiIPR014720. dsRNA-bd_dom.
    [Graphical view]
    PfamiPF00035. dsrm. 2 hits.
    [Graphical view]
    SMARTiSM00358. DSRM. 3 hits.
    [Graphical view]
    PROSITEiPS50137. DS_RBD. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4V8C7-1 [UniParc]FASTAAdd to Basket

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    MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGTKTKNIP    50
    VYECERSDVQ VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK 100
    ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE 150
    GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH 200
    ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD YIQLLSEIAK 250
    EQGFSITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH 300
    NALQYLKIIA ERK 313
    Length:313
    Mass (Da):34,355
    Last modified:July 5, 2005 - v1
    Checksum:i78550978D5B92043
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC097446 mRNA. Translation: AAH97446.1.
    RefSeqiNP_001019951.1. NM_001024780.1.
    UniGeneiRn.3217.

    Genome annotation databases

    GeneIDi311130.
    KEGGirno:311130.
    UCSCiRGD:1306707. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC097446 mRNA. Translation: AAH97446.1 .
    RefSeqi NP_001019951.1. NM_001024780.1.
    UniGenei Rn.3217.

    3D structure databases

    ProteinModelPortali Q4V8C7.
    SMRi Q4V8C7. Positions 28-104, 127-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015624.

    PTM databases

    PhosphoSitei Q4V8C7.

    Proteomic databases

    PaxDbi Q4V8C7.
    PRIDEi Q4V8C7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 311130.
    KEGGi rno:311130.
    UCSCi RGD:1306707. rat.

    Organism-specific databases

    CTDi 8575.
    RGDi 1306707. Prkra.

    Phylogenomic databases

    eggNOGi NOG245126.
    HOGENOMi HOG000231919.
    HOVERGENi HBG001700.
    InParanoidi Q4V8C7.
    PhylomeDBi Q4V8C7.

    Miscellaneous databases

    NextBioi 663036.
    PROi Q4V8C7.

    Gene expression databases

    Genevestigatori Q4V8C7.

    Family and domain databases

    Gene3Di 3.30.160.20. 3 hits.
    InterProi IPR014720. dsRNA-bd_dom.
    [Graphical view ]
    Pfami PF00035. dsrm. 2 hits.
    [Graphical view ]
    SMARTi SM00358. DSRM. 3 hits.
    [Graphical view ]
    PROSITEi PS50137. DS_RBD. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
      Moser K., White F.M.
      J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRKRA_RAT
    AccessioniPrimary (citable) accession number: Q4V8C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2006
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3