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Protein

Centromere/kinetochore protein zw10 homolog

Gene

Zw10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, ER-Golgi transport, Mitosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere/kinetochore protein zw10 homolog
Gene namesi
Name:Zw10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1309197. Zw10.

Subcellular locationi

  • Cytoplasm By similarity
  • Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
  • Chromosomecentromerekinetochore By similarity
  • Cytoplasmcytoskeletonspindle By similarity

  • Note: Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Kinetochore, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 777776Centromere/kinetochore protein zw10 homologPRO_0000184959Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Modified residuei775 – 7751N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ4V8C2.
PRIDEiQ4V8C2.

PTM databases

iPTMnetiQ4V8C2.
PhosphoSiteiQ4V8C2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007661.
GenevisibleiQ4V8C2. RN.

Interactioni

Subunit structurei

Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25 and ZWINT STOPPED Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1 and ZWINT.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010595.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 316315Interaction with RINT1By similarityAdd
BLAST
Regioni2 – 8180Interaction with ZWINTBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili14 – 130117Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the ZW10 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2163. Eukaryota.
ENOG410XQ7J. LUCA.
GeneTreeiENSGT00390000016427.
HOGENOMiHOG000007982.
HOVERGENiHBG004603.
InParanoidiQ4V8C2.
KOiK11578.
OMAiIRFESVM.
OrthoDBiEOG091G026L.
PhylomeDBiQ4V8C2.
TreeFamiTF105966.

Family and domain databases

InterProiIPR009361. RZZ-complex_Zw10.
[Graphical view]
PfamiPF06248. Zw10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V8C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASFVTEVLA HSGSLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL
60 70 80 90 100
PTMQSAQALV TQVDTLSNDI DQLKSRIETE VCRDLHISTV EFTNLKQRLE
110 120 130 140 150
RDSVVLNLLK QLQEFSSAIE EYNSALAEKK YIPAARLLEE AQECLKLLKS
160 170 180 190 200
KKCFDLKMLK SLSMELTVQK QNILYHLGED WQKLVVWKFP PSKDTSSLES
210 220 230 240 250
CLQTELHLCT EQPEKEMTPL PSISSVLLAF SILGELPTKL KSFGQMLLKY
260 270 280 290 300
ILKPLVTCPS LHAVIERQPN SVSICFQSLA TDSEHPPPPE AFAKIQLVLE
310 320 330 340 350
VLQKQLLDLP LDADLEIGKV PEIVLAEMLG EVIWEDLSDC LIRNCLVYSI
360 370 380 390 400
PTNSSKLQQY EEIIQSTEEF EKSLKEMRFL KGDTTDLLKY ARNINSHFAN
410 420 430 440 450
KKCQDVIVAA RHLMTSEIHN TVKIGPDCEE TLPDLPSPDA DHRLQVQVCK
460 470 480 490 500
VQFTDAGNLE PETSLDPRSF SLPTCRISEA VKKLMELAYQ TLLEATTSSD
510 520 530 540 550
QCAVQLFYSV RNIFHLFHDV VPTYHKENLQ KLPQLAAIHH NNCMYIAHHL
560 570 580 590 600
LTLGHQFRSR LTPILCDGTT TFVDLVPGFR RLGTECFLAQ MRTQKGELLE
610 620 630 640 650
RLSSARSFAN MDDEENYSAA SKAVRQVLHQ LKRLGIVWQD VLPVNIYCKA
660 670 680 690 700
MGTLLNTVIA EMIGRITALE DISTEDGDRL YSLCKTVMDE GPQVFAPLSD
710 720 730 740 750
ENKNKKYQEE VPVYVSKWMP FKELMIMLQA SLQEIGDRWA DGKGPLATAF
760 770
PSSEVKALIR ALFQNTERRA AALAKIK
Length:777
Mass (Da):87,967
Last modified:January 23, 2007 - v3
Checksum:iC56BB79891D4C691
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097452 mRNA. Translation: AAH97452.1.
RefSeqiNP_001019972.1. NM_001024801.1.
UniGeneiRn.25242.

Genome annotation databases

EnsembliENSRNOT00000080712; ENSRNOP00000071533; ENSRNOG00000054479.
GeneIDi363059.
KEGGirno:363059.
UCSCiRGD:1309197. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097452 mRNA. Translation: AAH97452.1.
RefSeqiNP_001019972.1. NM_001024801.1.
UniGeneiRn.25242.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010595.

PTM databases

iPTMnetiQ4V8C2.
PhosphoSiteiQ4V8C2.

Proteomic databases

PaxDbiQ4V8C2.
PRIDEiQ4V8C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000080712; ENSRNOP00000071533; ENSRNOG00000054479.
GeneIDi363059.
KEGGirno:363059.
UCSCiRGD:1309197. rat.

Organism-specific databases

CTDi9183.
RGDi1309197. Zw10.

Phylogenomic databases

eggNOGiKOG2163. Eukaryota.
ENOG410XQ7J. LUCA.
GeneTreeiENSGT00390000016427.
HOGENOMiHOG000007982.
HOVERGENiHBG004603.
InParanoidiQ4V8C2.
KOiK11578.
OMAiIRFESVM.
OrthoDBiEOG091G026L.
PhylomeDBiQ4V8C2.
TreeFamiTF105966.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ4V8C2.

Gene expression databases

BgeeiENSRNOG00000007661.
GenevisibleiQ4V8C2. RN.

Family and domain databases

InterProiIPR009361. RZZ-complex_Zw10.
[Graphical view]
PfamiPF06248. Zw10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZW10_RAT
AccessioniPrimary (citable) accession number: Q4V8C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.