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Protein

Dual specificity tyrosine-phosphorylation-regulated kinase 3

Gene

Dyrk3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Negative regulator of EPO-dependent erythropoiesis, may place an upper limit on red cell production during stress erythropoiesis. Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (By similarity). May act by regulating CREB/CRE signaling (By similarity). Stabilizes and prevents stress granule disassembly thereby regulating mTORC1 signaling during cellular stress. During stressful conditions, DYRK3 partitions to the stress granule from the cytosol, as well as mTORC1 components, which prevents mTORC1 signaling. When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol, and promotes the phosphorylation of the mTORC1 inhibitor, AKT1S1, allowing full reactivation of mTORC1 signaling. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei237ATPPROSITE-ProRule annotationBy similarity1
Active sitei334Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi214 – 222ATPPROSITE-ProRule annotationBy similarity9
Nucleotide bindingi287 – 290ATPPROSITE-ProRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity tyrosine-phosphorylation-regulated kinase 3 (EC:2.7.12.1By similarity)
Gene namesi
Name:Dyrk3Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1310924. Dyrk3.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmic granule By similarity

  • Note: Shuttles between cytoplasm and stress granules. Localized predominantly on distinct speckles distributed throughout the cytoplasm of the cell. At low concentration, showns a homogeneous distribution throughout the cytoplasm and does not condense in speckles. During oxidative and osmotic stress, localizes to stress granules.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002915381 – 586Dual specificity tyrosine-phosphorylation-regulated kinase 3Add BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei368PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated on tyrosine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4V8A3.
PRIDEiQ4V8A3.

PTM databases

iPTMnetiQ4V8A3.
PhosphoSitePlusiQ4V8A3.

Expressioni

Tissue specificityi

Expressed predominantly in testis (PubMed:9748265). Expressed in late pachytene spermatocytes (PubMed:17292540).2 Publications

Gene expression databases

BgeeiENSRNOG00000004870.

Interactioni

Subunit structurei

Interacts with SIRT1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006502.

Structurei

3D structure databases

ProteinModelPortaliQ4V8A3.
SMRiQ4V8A3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 521Protein kinasePROSITE-ProRule annotationAdd BLAST314

Domaini

The N-terminal domain is required for stress granule localization.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOGENOMiHOG000220863.
HOVERGENiHBG051426.
InParanoidiQ4V8A3.
KOiK18669.
PhylomeDBiQ4V8A3.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4V8A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGAARERGR KDAALPGAGL PPQQRRLGDG VYDTFMMIDE TKCPPYTNTL
60 70 80 90 100
CNPSEAPVSR RLNITTEPFT RGHTQHFVSG GVMKVEQLFQ EFGSRRTSTL
110 120 130 140 150
QSDGVSNSEK SSPASQGKSS DSLGTVKCSL SSRPSKVLPL TPEQALKQYK
160 170 180 190 200
HHLTAYEKLE IISYPEIYFV GPNAKKRQGV IGGPNNGGYD DADGAYIHVP
210 220 230 240 250
RDHLAYRYEV LKIIGKGSFG QVARVYDHKL RQYVALKMVR NEKRFHRQAA
260 270 280 290 300
EEIRILEHLK KQDKTGSMNV IHMLESFTFR NHVCMAFELL SIDLYELIKK
310 320 330 340 350
NKFQGFSVQL VRKFAQSILQ SLDALHKNKI IHCDLKPENI LLKHHGRSAT
360 370 380 390 400
KVIDFGSSCF EYQKLYTYIQ SRFYRAPEII LGCRYSTPID IWSFGCILAE
410 420 430 440 450
LLTGQPLFPG EDEGDQLACM MELLGMPPQK LLEQSKRAKY FINSKGLPRY
460 470 480 490 500
CSVTTQTDGR VVLLGGRSRR GKKRGPPGSK DWAAALKGCD DYLFIEFLKR
510 520 530 540 550
CLQWDPSARL TPAQALRHPW ISKSAPRPLT TDKVSGKRVV NPTNAFQGLG
560 570 580
SKLPPVVGIA SKLKANLMSE TSGSIPLCSV LPKLIS
Length:586
Mass (Da):65,511
Last modified:July 5, 2005 - v1
Checksum:i9E194D2F960FBE94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097474 mRNA. Translation: AAH97474.1.
RefSeqiNP_001019938.1. NM_001024767.1.
UniGeneiRn.162390.

Genome annotation databases

GeneIDi304775.
KEGGirno:304775.
UCSCiRGD:1310924. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097474 mRNA. Translation: AAH97474.1.
RefSeqiNP_001019938.1. NM_001024767.1.
UniGeneiRn.162390.

3D structure databases

ProteinModelPortaliQ4V8A3.
SMRiQ4V8A3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006502.

PTM databases

iPTMnetiQ4V8A3.
PhosphoSitePlusiQ4V8A3.

Proteomic databases

PaxDbiQ4V8A3.
PRIDEiQ4V8A3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi304775.
KEGGirno:304775.
UCSCiRGD:1310924. rat.

Organism-specific databases

CTDi8444.
RGDi1310924. Dyrk3.

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOGENOMiHOG000220863.
HOVERGENiHBG051426.
InParanoidiQ4V8A3.
KOiK18669.
PhylomeDBiQ4V8A3.

Miscellaneous databases

PROiPR:Q4V8A3.

Gene expression databases

BgeeiENSRNOG00000004870.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDYRK3_RAT
AccessioniPrimary (citable) accession number: Q4V8A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 5, 2005
Last modified: May 10, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.