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Protein

Cell division cycle protein 27 homolog

Gene

Cdc27

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-RNO-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-RNO-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-RNO-174154 APC/C:Cdc20 mediated degradation of Securin
R-RNO-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-RNO-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-RNO-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-RNO-176408 Regulation of APC/C activators between G1/S and early anaphase
R-RNO-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-RNO-176412 Phosphorylation of the APC/C
R-RNO-179409 APC-Cdc20 mediated degradation of Nek2A
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-RNO-69017 CDK-mediated phosphorylation and removal of Cdc6
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 27 homolog
Gene namesi
Name:Cdc27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1304921 Cdc27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904741 – 824Cell division cycle protein 27 homologAdd BLAST824

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205PhosphothreonineBy similarity1
Modified residuei209PhosphothreonineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei313PhosphothreonineBy similarity1
Modified residuei339PhosphoserineBy similarity1
Modified residuei367PhosphothreonineBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei387PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei431PhosphothreonineBy similarity1
Modified residuei436PhosphoserineBy similarity1
Modified residuei439PhosphoserineBy similarity1
Modified residuei447PhosphothreonineBy similarity1
Modified residuei821PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation on Ser-427 and Thr-447 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4V8A2
PRIDEiQ4V8A2

PTM databases

iPTMnetiQ4V8A2
PhosphoSitePlusiQ4V8A2

Expressioni

Gene expression databases

BgeeiENSRNOG00000005904
GenevisibleiQ4V8A2 RN

Interactioni

Subunit structurei

The APC/C is composed of at least 12 subunits. Interacts with RB (By similarity). Interacts with MCPH1 (By similarity). Interacts with FAM168B/MANI (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi262095, 5 interactors
IntActiQ4V8A2, 1 interactor
MINTiQ4V8A2
STRINGi10116.ENSRNOP00000007963

Structurei

3D structure databases

ProteinModelPortaliQ4V8A2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati84 – 114TPR 1Add BLAST31
Repeati115 – 148TPR 2Add BLAST34
Repeati499 – 532TPR 3Add BLAST34
Repeati567 – 600TPR 4Add BLAST34
Repeati602 – 634TPR 5Add BLAST33
Repeati635 – 668TPR 6Add BLAST34
Repeati670 – 702TPR 7Add BLAST33
Repeati704 – 736TPR 8Add BLAST33
Repeati737 – 770TPR 9Add BLAST34

Sequence similaritiesi

Belongs to the APC3/CDC27 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1126 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00550000074887
HOGENOMiHOG000231056
HOVERGENiHBG050859
InParanoidiQ4V8A2
KOiK03350
OMAiMPLTSPC
OrthoDBiEOG091G03JF

Family and domain databases

Gene3Di1.25.40.10, 4 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 2 hits
PF13181 TPR_8, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 8 hits
PS50293 TPR_REGION, 2 hits

Sequencei

Sequence statusi: Complete.

Q4V8A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR
60 70 80 90 100
SGKAYKAYRL LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ
110 120 130 140 150
KSHDDLVTEF GDSACFTLSL LGHVYCKTDR LAKGSECYQK SLSLNPFLWS
160 170 180 190 200
PFESLCEIGE KPDPDQTFKL TSLQNFSSCL PNTCTALVSN HSLSHRQPET
210 220 230 240 250
VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSAVI SPDNVPLGTG
260 270 280 290 300
PSILSKQVQN KPKTGRSLLG GPTALSPLTP SFGILPLETP SPGDGSYLQN
310 320 330 340 350
YTNTPSVIDV PSTGAPTKKS VARMGHTGAK SVFSQSGNSR EATPVLVAQT
360 370 380 390 400
QSSGPQTSTT PQVLSPTITS PPNALPRRSS RLFTSDSSTT KENSKKLKMK
410 420 430 440 450
FPPKIPNRKT KSKTNKGGIT QPNINDSLEI TKLDSSIISE GKISTITPQI
460 470 480 490 500
QAFNLQKAAA GLMSLLREMG KGYLALCSYN CKEAINILSH LPSHHYSTGW
510 520 530 540 550
VLCQIGRAYF ELSEYMQAER IFSEVRRIES FRVEGMEIYS TTLWHLQKDV
560 570 580 590 600
ALSVLSKDLT DMDKNSPEAW CAAGNCFSLQ REHDIAIKFF QRAIQVDPNY
610 620 630 640 650
AYAYTLLGHE FVLTEELDKA LACFRNAIRV NPRHYNAWYG LGMIYYKQEK
660 670 680 690 700
FSLAEMHFQK ALDINPQSSV LLCHIGVVQH ALKKSEKALD TLNKAIVIDP
710 720 730 740 750
KNPLCKFHRA SVLFANEKYK SALQELEELK QIVPKESLVY FLIGKVYKKL
760 770 780 790 800
GQTHLALMNF SWAMDLDPKG ANNQIKEAID KRYLPDDEEP ITQEEQIMGT
810 820
DESQESSMTD ADDTQLHAAE SDEF
Length:824
Mass (Da):91,663
Last modified:July 5, 2005 - v1
Checksum:i24B02A896A0463A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097475 mRNA Translation: AAH97475.1
RefSeqiNP_001019964.1, NM_001024793.1
UniGeneiRn.198335

Genome annotation databases

EnsembliENSRNOT00000007963; ENSRNOP00000007963; ENSRNOG00000005904
GeneIDi360643
KEGGirno:360643
UCSCiRGD:1304921 rat

Similar proteinsi

Entry informationi

Entry nameiCDC27_RAT
AccessioniPrimary (citable) accession number: Q4V8A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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