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Protein

E3 SUMO-protein ligase NSE2

Gene

Nsmce2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. Required for sister chromatid cohesion during prometaphase and mitotic progression (By similarity).By similarity

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri154 – 236SP-RING-typePROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, Mitosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-3108214. SUMOylation of DNA damage response and repair proteins.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase NSE2 (EC:6.3.2.-)
Alternative name(s):
MMS21 homolog
Non-structural maintenance of chromosomes element 2 homolog
Short name:
Non-SMC element 2 homolog
Gene namesi
Name:Nsmce2
Synonyms:Mms21
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1305156. Nsmce2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002709411 – 247E3 SUMO-protein ligase NSE2Add BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki90Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki107Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei116PhosphoserineBy similarity1
Cross-linki125Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated, possibly via autosumoylation.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4V8A0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003964.
ExpressionAtlasiQ4V8A0. baseline and differential.
GenevisibleiQ4V8A0. RN.

Interactioni

Subunit structurei

Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005376.

Structurei

3D structure databases

ProteinModelPortaliQ4V8A0.
SMRiQ4V8A0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NSE2 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri154 – 236SP-RING-typePROSITE-ProRule annotationAdd BLAST83

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2979. Eukaryota.
COG5627. LUCA.
GeneTreeiENSGT00390000013961.
HOGENOMiHOG000063700.
HOVERGENiHBG082059.
InParanoidiQ4V8A0.
OMAiMIESKHR.
OrthoDBiEOG091G11GG.
PhylomeDBiQ4V8A0.
TreeFamiTF324383.

Family and domain databases

CDDicd16651. SPL-RING_NSE2. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR026846. Nse2(Mms21).
IPR003613. Ubox_domain.
IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR21330. PTHR21330. 1 hit.
PfamiView protein in Pfam
PF11789. zf-Nse. 1 hit.
SMARTiView protein in SMART
SM00504. Ubox. 1 hit.
PROSITEiView protein in PROSITE
PS51044. ZF_SP_RING. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4V8A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGRSSTNSG STRYISFSGV ESALSSLKTF QSCISSGMDT VSSVALDLVE
60 70 80 90 100
TQTEVSSEYS MDKAMVEFAK MDRELNHYVK AVQSTINHVK EERPEKVPDL
110 120 130 140 150
KLLVEKKFLA LQDKNSDADF KENEKFVQFK QQLRELKKQY GIHADRENDG
160 170 180 190 200
IEGMDEDMIV TQSQTNFICP ITQLEMKKPV KNKMCGHTYE EEAIVRMIES
210 220 230 240
KHKRKKKACC PKIGCSHTDM RMSDLIPDEA LRRAIESHNK KKKRHSE
Length:247
Mass (Da):28,246
Last modified:July 5, 2005 - v1
Checksum:i5868B52733255F80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097477 mRNA. Translation: AAH97477.1.
RefSeqiNP_001020047.1. NM_001024876.1.
UniGeneiRn.203454.

Genome annotation databases

EnsembliENSRNOT00000005376; ENSRNOP00000005376; ENSRNOG00000003964.
GeneIDi299957.
KEGGirno:299957.
UCSCiRGD:1305156. rat.

Similar proteinsi

Entry informationi

Entry nameiNSE2_RAT
AccessioniPrimary (citable) accession number: Q4V8A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 5, 2005
Last modified: August 30, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families