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Protein

RNA-binding motif protein, X chromosome

Gene

Rbmx

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.3 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • single-stranded RNA binding Source: RGD

GO - Biological processi

  • mRNA splice site selection Source: RGD
  • positive regulation of transcription, DNA-templated Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding motif protein, X chromosome
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein G
Short name:
hnRNP G
RNA-binding motif protein, X chromosome retrogene
RNA-binding motif protein, X chromosome retrogene-like
Cleaved into the following chain:
Gene namesi
Name:Rbmx
Synonyms:Rbmxrt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi1565256. Rbmx.

Subcellular locationi

  • Nucleus By similarity

  • Note: Component of ribonucleosomes (By similarity). Localizes in numerous small granules in the nucleus.By similarity

GO - Cellular componenti

  • nucleus Source: RGD
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390RNA-binding motif protein, X chromosomePRO_0000413089Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 390389RNA-binding motif protein, X chromosome, N-terminally processedPRO_0000413017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein G; alternateBy similarity
Modified residuei2 – 21N-acetylvaline; in Heterogeneous nuclear ribonucleoprotein G, N-terminally processedBy similarity
Modified residuei30 – 301N6-acetyllysineBy similarity
Cross-linki80 – 80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei88 – 881PhosphoserineBy similarity
Modified residuei91 – 911PhosphoserineBy similarity
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei174 – 1741PhosphoserineBy similarity
Modified residuei185 – 1851Omega-N-methylated arginineBy similarity
Modified residuei261 – 2611PhosphoserineBy similarity
Modified residuei329 – 3291PhosphoserineBy similarity
Modified residuei330 – 3301PhosphoserineBy similarity
Modified residuei332 – 3321PhosphoserineBy similarity
Modified residuei352 – 3521PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated.By similarity
Arg-182 is dimethylated, probably to asymmetric dimethylarginine.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4V898.
PRIDEiQ4V898.

PTM databases

iPTMnetiQ4V898.
PhosphoSiteiQ4V898.

Expressioni

Tissue specificityi

Expressed in brain, spleen, lung, liver, kidney, testis and heart. Weakly expressed in skeletal muscle (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000000866.
GenevisibleiQ4V898. RN.

Interactioni

Subunit structurei

Found in the spliceosome C complex. Interacts with KHDRBS3. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, NCOA5 and PPP1CA. Interacts with SAFB/SAFB1. Interacts with ERAP1; the interaction is RNA-independent (By similarity). Homomultimer. Found in the supraspliceosome complex. Interacts with CLK2, KHDRBS2, SAFB, TRA2B and YTHDC1.By similarity1 Publication

Protein-protein interaction databases

BioGridi257324. 1 interaction.
STRINGi10116.ENSRNOP00000001154.

Structurei

3D structure databases

ProteinModelPortaliQ4V898.
SMRiQ4V898. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 8679RRMPROSITE-ProRule annotationAdd
BLAST

Domaini

The RRM domain is necessary for RNA-binding, but not for splice site selection, indicating that its splicing activity does not require direct binding to RNA.

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHG3. Eukaryota.
ENOG4111GI6. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276235.
HOVERGENiHBG063314.
InParanoidiQ4V898.
KOiK12885.
OMAiRDYSYRE.
OrthoDBiEOG091G13K0.
PhylomeDBiQ4V898.
TreeFamiTF331833.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012604. RBM1CTR.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF08081. RBM1CTR. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG
60 70 80 90 100
FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRGLPPPP
110 120 130 140 150
RSRGPPRGLR GGRGGSGGTR GPPSRGGHMD DGGYSMNFTL SSSRGPLPVK
160 170 180 190 200
RGPPPRSGGP PPKRSAPSGP VRSSSGMGGR APVSRGRDGY GGPPRREPLP
210 220 230 240 250
SRRDVYLSPR DDGYSTKDSY SSRDYPSSRD TRDYAPPPRD YTYRDYGHSS
260 270 280 290 300
SRDDYPSRGY SDRDGYGRER DYSDHPSGGS YRDSYESYGN SRSAPPTRGP
310 320 330 340 350
PPSYGGSSRY DDYSSSRDGY GGSRDSYTSS RSDLYSSGRD RVGRQERGLP
360 370 380 390
PSMERGYPPP RDSYSSSSRG APRGGGRGGS RSDRGGRSRY
Length:390
Mass (Da):42,258
Last modified:July 5, 2005 - v1
Checksum:i55DFD2163C9D4A9E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121I → K AA sequence (PubMed:16128803).Curated
Sequence conflicti348 – 3481G → D AA sequence (PubMed:16128803).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474106 Genomic DNA. Translation: EDL75123.1.
BC097479 mRNA. Translation: AAH97479.1.
RefSeqiNP_001020834.1. NM_001025663.1.
XP_006257742.1. XM_006257680.2.
XP_006257743.1. XM_006257681.2.
XP_006257744.1. XM_006257682.2.
XP_006257745.1. XM_006257683.2.
UniGeneiRn.229269.

Genome annotation databases

EnsembliENSRNOT00000001154; ENSRNOP00000001154; ENSRNOG00000000866.
GeneIDi302855.
KEGGirno:302855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474106 Genomic DNA. Translation: EDL75123.1.
BC097479 mRNA. Translation: AAH97479.1.
RefSeqiNP_001020834.1. NM_001025663.1.
XP_006257742.1. XM_006257680.2.
XP_006257743.1. XM_006257681.2.
XP_006257744.1. XM_006257682.2.
XP_006257745.1. XM_006257683.2.
UniGeneiRn.229269.

3D structure databases

ProteinModelPortaliQ4V898.
SMRiQ4V898. Positions 1-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi257324. 1 interaction.
STRINGi10116.ENSRNOP00000001154.

PTM databases

iPTMnetiQ4V898.
PhosphoSiteiQ4V898.

Proteomic databases

PaxDbiQ4V898.
PRIDEiQ4V898.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001154; ENSRNOP00000001154; ENSRNOG00000000866.
GeneIDi302855.
KEGGirno:302855.

Organism-specific databases

CTDi27316.
RGDi1565256. Rbmx.

Phylogenomic databases

eggNOGiENOG410IHG3. Eukaryota.
ENOG4111GI6. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276235.
HOVERGENiHBG063314.
InParanoidiQ4V898.
KOiK12885.
OMAiRDYSYRE.
OrthoDBiEOG091G13K0.
PhylomeDBiQ4V898.
TreeFamiTF331833.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ4V898.

Gene expression databases

BgeeiENSRNOG00000000866.
GenevisibleiQ4V898. RN.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012604. RBM1CTR.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF08081. RBM1CTR. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBMX_RAT
AccessioniPrimary (citable) accession number: Q4V898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.