Q4V863 (EZH2B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase EZH2
EC=2.1.1.43

Alternative name(s):
Enhancer of zeste homolog 2-B

Gene names

Name:

ezh2-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polycomb group (PcG) protein By similarity. Catalytic subunit of the prc2/eed-ezh2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subunit structure	Component of the prc2/eed-ezh2 complex By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ESC/E(Z) complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Histone-lysine N-methyltransferase EZH2		PRO_0000345429
Regions
	Domain	613 – 733	121	SET
	Compositional bias	183 – 207	25	Asp-rich
	Compositional bias	525 – 590	66	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q4V863 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: BD6B17AF6B7B2A6A	FASTA	748	85,742
	10 20 30 40 50 60 MGQTGKKSEK GAVCWRKRVK SEYMRLRQLK RFRRADEVKS MFNTNRQKIM ERTEILNQEW 70 80 90 100 110 120 KQRRIQPVHI MTTVSSLRGT RECFVTSDLD FPKQVIPLKT LTAVASMPIM YSWSPLQQNF 130 140 150 160 170 180 MVEDETVLHN IPYMGDEVLD QDGTFIEELI KNYDGKVHGD RECGFINDEI FVELVNALAQ 190 200 210 220 230 240 YSDYEDDEDG EDNQDDERDD ITKDQDDNME EKETLPLRKF PSDKIFEAIS SVFPDKGTSE 250 260 270 280 290 300 ELKEKYKELT EQQLPGALPP ECTPNIDGSN AKSVQREQSL HSFHTLFCRR CFKYDCFLHP 310 320 330 340 350 360 FHATPNTYKR KNNEAANDGK LCGPYCYQLL EGAREFAAAL TAEIIKTPPK RPSGRRRGRL 370 380 390 400 410 420 PNNSSRPSTP TVNVLEAKDT DSDREAGTET GGESNDKEEE EKKDETDSSS EANSRCQTPI 430 440 450 460 470 480 KMKPNIEPPE NVEWSGAEAS LFRVLIGTYY DNFCAIARLI STKTCRQVYE FRVKESSIIA 490 500 510 520 530 540 PVIAEDVDTP PRKKKRKHRL WAAHCRKIQL KKDGSSNHVY NYQPCDHPRQ PCDSSCPCVI 550 560 570 580 590 600 AQNFCEKFCQ CSSDCQNRFP GCRCKAQCNT KQCPCYLAVR ECDPDLCLTC GAADHWDSKN 610 620 630 640 650 660 VSCKNCSIQR GSKKHLLLAP SDVAGWGIYI KDPVQKNEFI SEYCGEIISQ DEADRRGKVY 670 680 690 700 710 720 DKYMCSFLFN LNNDFVVDAT RKGNKIRFAN HSLNPNCYAK VMMVNGDHRI GIFAKRAIQT 730 740 GEELFFDYRY SQADALKYVG IEREMEIP

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC097526 mRNA. Translation: AAH97526.1.
RefSeq	NP_001167506.1. NM_001174035.1.
UniGene	Xl.47646.
3D structure databases
HSSP	HSSP built from PDB template 1ML9 based on UniProtKB Q8X225.
ProteinModelPortal	Q4V863.
SMR	Q4V863. Positions 40-68.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100381148.
KEGG	xla:100381148.
Organism-specific databases
CTD	100381148.
Xenbase	XB-GENE-6252001. ezh2.
Phylogenomic databases
HOVERGEN	HBG002453.
Family and domain databases
InterPro	IPR021654. EZH2_WD-Binding. IPR001005. SANT_DNA-bd. IPR001214. SET_dom. [Graphical view]
Pfam	PF11616. EZH2_WD-Binding. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 2 hits. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZH2B_XENLA

Accession

Primary (citable) accession number: Q4V863

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 5, 2005
Last modified:	September 21, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program