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Protein

tRNA (cytosine(34)-C(5))-methyltransferase

Gene

nsun2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.

Enzyme regulationi

Inhibited by magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei213S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei240S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei266S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei319NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase (EC:2.1.1.203)
Alternative name(s):
NOL1/NOP2/Sun domain family member 2
Gene namesi
Name:nsun2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-962628. nsun2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892261 – 698tRNA (cytosine(34)-C(5))-methyltransferaseAdd BLAST698

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ4V7N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 188S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG106711.
KOiK15335.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V7N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKNRRNRQ RRTEQRSPAE EERRKAREQA AWEGGYPEII KENKLFEHYY
60 70 80 90 100
QELKIVPDGE WDKFMDALRE PLPATIRITG YKSHAKEILH CLKEKYFKEL
110 120 130 140 150
PDIEVDGQKI EAPQPLSWYP EELAWHTNLS RKIIRKSPEL EKFHQFLVSE
160 170 180 190 200
TESGNISRQE AVSMIPPVLL NVQPHHKILD MCAAPGSKTA QIIEMLHADM
210 220 230 240 250
NVPFPEGFVI ANDVDNKRCY LLVHQAKRLN SPCIMVVNHD ASSIPRLLIE
260 270 280 290 300
NNGSREVLYY DRILCDVPCS GDGTMRKNID VWKKWTTLNS LQLHGLQIRI
310 320 330 340 350
ATRGVEQLAE GGRMVYSTCS LNPVEDEAVI VSLLDKSEGS LELADVASEL
360 370 380 390 400
PGLKWMPGIT QWRVMTKEGQ WFEKWEDVPT SRHTQIRPTM FPLKDEEKLK
410 420 430 440 450
SMNLNRCMRI LPHHQNTGGF FVAVLIKKAP MPWNKRQPKL QRRPPVSVCD
460 470 480 490 500
ASVAPEIVKA VADISAIADE PAVDAENGET KPCTNQSGSS KTDSVCCPPP
510 520 530 540 550
SKKMKLFGFK EDPFVFLSED DPIFEPIQKF YALDPSFPKK NLLTRTQEGK
560 570 580 590 600
KRQLYMVSKE LRNVLLHNSE KMKVINTGIK VLCRNNDGEQ YGCAYRLAQE
610 620 630 640 650
GIYSLYPFIN ARILTVSVED IKVLLTQENP FLSKFSKETQ KQANNLDMGS
660 670 680 690
IVLKYEPDPQ QPETLQCPIV LCGWRGKTSI RSFVFFLHRI NLVQWIFI
Length:698
Mass (Da):80,051
Last modified:July 5, 2005 - v1
Checksum:i3AD1F0DEF6CCBB43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097814 mRNA. Translation: AAH97814.1.
RefSeqiNP_001084513.1. NM_001091044.1.
UniGeneiXl.24071.

Genome annotation databases

GeneIDi414460.
KEGGixla:414460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097814 mRNA. Translation: AAH97814.1.
RefSeqiNP_001084513.1. NM_001091044.1.
UniGeneiXl.24071.

3D structure databases

ProteinModelPortaliQ4V7N2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi414460.
KEGGixla:414460.

Organism-specific databases

CTDi54888.
XenbaseiXB-GENE-962628. nsun2.

Phylogenomic databases

HOVERGENiHBG106711.
KOiK15335.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN2_XENLA
AccessioniPrimary (citable) accession number: Q4V7N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2005
Last modified: May 11, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.