Reviewed, UniProtKB/Swiss-Prot Q4V7N2 (NSUN2_XENLA)

Names and origin

Protein names

Recommended name:
    tRNA (cytosine-5-)-methyltransferase NSUN2
    EC=2.1.1.29
Alternative name(s):
    NOL1/NOP2/Sun domain family member 2

Gene names

Name:

nsun2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus › nucleolus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	tRNA (cytosine-5-)-methyltransferase NSUN2		PRO_0000289226
Regions
	Region	182 – 188	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	319	1	Nucleophile Potential
	Binding site	213	1	S-adenosyl-L-methionine By similarity
	Binding site	240	1	S-adenosyl-L-methionine By similarity
	Binding site	266	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4V7N2-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 3AD1F0DEF6CCBB43	FASTA	698	80,051
	10 20 30 40 50 60 MGRKNRRNRQ RRTEQRSPAE EERRKAREQA AWEGGYPEII KENKLFEHYY QELKIVPDGE 70 80 90 100 110 120 WDKFMDALRE PLPATIRITG YKSHAKEILH CLKEKYFKEL PDIEVDGQKI EAPQPLSWYP 130 140 150 160 170 180 EELAWHTNLS RKIIRKSPEL EKFHQFLVSE TESGNISRQE AVSMIPPVLL NVQPHHKILD 190 200 210 220 230 240 MCAAPGSKTA QIIEMLHADM NVPFPEGFVI ANDVDNKRCY LLVHQAKRLN SPCIMVVNHD 250 260 270 280 290 300 ASSIPRLLIE NNGSREVLYY DRILCDVPCS GDGTMRKNID VWKKWTTLNS LQLHGLQIRI 310 320 330 340 350 360 ATRGVEQLAE GGRMVYSTCS LNPVEDEAVI VSLLDKSEGS LELADVASEL PGLKWMPGIT 370 380 390 400 410 420 QWRVMTKEGQ WFEKWEDVPT SRHTQIRPTM FPLKDEEKLK SMNLNRCMRI LPHHQNTGGF 430 440 450 460 470 480 FVAVLIKKAP MPWNKRQPKL QRRPPVSVCD ASVAPEIVKA VADISAIADE PAVDAENGET 490 500 510 520 530 540 KPCTNQSGSS KTDSVCCPPP SKKMKLFGFK EDPFVFLSED DPIFEPIQKF YALDPSFPKK 550 560 570 580 590 600 NLLTRTQEGK KRQLYMVSKE LRNVLLHNSE KMKVINTGIK VLCRNNDGEQ YGCAYRLAQE 610 620 630 640 650 660 GIYSLYPFIN ARILTVSVED IKVLLTQENP FLSKFSKETQ KQANNLDMGS IVLKYEPDPQ 670 680 690 QPETLQCPIV LCGWRGKTSI RSFVFFLHRI NLVQWIFI

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC097814 mRNA. Translation: AAH97814.1.
RefSeq	NP_001084513.1.
UniGene	Xl.24071
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	414460.
KEGG	xla:414460.
Organism-specific databases
CTD	414460.
Xenbase	XB-FEAT-962622. nsun2.
Phylogenomic databases
HOVERGEN	Q4V7N2.
Enzyme and pathway databases
BRENDA	2.1.1.29. 648.
Family and domain databases
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 2 hits. [Graphical view]
PROSITE	PS01153. NOL1_NOP2_SUN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSUN2_XENLA

Accession

Primary (citable) accession number: Q4V7N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2005
Last modified:	September 1, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project