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Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Gene

Osgepl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi147Divalent metal cationUniRule annotation1
Metal bindingi151Divalent metal cationUniRule annotation1
Binding sitei202SubstrateUniRule annotation1
Binding sitei222Substrate; via amide nitrogenUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei357SubstrateUniRule annotation1
Metal bindingi358Divalent metal cationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
O-sialoglycoprotein endopeptidase-like protein 1UniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Osgepl1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Osgepl1UniRule annotation
Gene namesi
Name:Osgepl1UniRule annotation
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1305575. Osgepl1.

Subcellular locationi

  • Mitochondrion UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionUniRule annotationAdd BLAST29
ChainiPRO_000030778030 – 414Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialAdd BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei299N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ4V7F3.
PRIDEiQ4V7F3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004001.
GenevisibleiQ4V7F3. RN.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005408.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 173Substrate bindingUniRule annotation5
Regioni329 – 330Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
GeneTreeiENSGT00550000075001.
HOGENOMiHOG000109570.
HOVERGENiHBG108218.
InParanoidiQ4V7F3.
KOiK01409.
OMAiICIDHIL.
OrthoDBiEOG091G0HJR.
PhylomeDBiQ4V7F3.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V7F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMLSKTAGA IPRPPRSNVR GFIRRFNVQP RALFHHKLVL GIETSCDDTA
60 70 80 90 100
AAVVDETGNV LGEALHSQTE VHLKTGGIVP PVAQQLHREN IQRIVEEALS
110 120 130 140 150
ASGVSPSDLS AIATTIKPGL ALSLGVGLSF SVQLVNQFKK PFIPIHHMEA
160 170 180 190 200
HALTIRLTHK VGFPFLVLLI SGGHCLLALV QSVSDFLLLG KSLDIAPGDM
210 220 230 240 250
LDKVARRLSL IKHPECSTMS GGKAIEHLAK EGNRFHFTIN PPMQNAKNCD
260 270 280 290 300
FSFTGLQHVT DKLITHKEKE EGIEKGQILS SAADIAAAVQ HATACHLAKR
310 320 330 340 350
THRAILFCQQ KNLLSPANAV LVVSGGVASN LYIRRALEIV ANATQCTLLC
360 370 380 390 400
PPPRLCTDNG IMIAWNGIER LRAGLGILHD VEDIRYEPKC PLGIDISREV
410
AEAAIKVPRL KMTL
Length:414
Mass (Da):44,851
Last modified:July 5, 2005 - v1
Checksum:iDE3B69028E0AAF9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097950 mRNA. Translation: AAH97950.1.
RefSeqiNP_001019958.1. NM_001024787.1.
XP_017451887.1. XM_017596398.1.
UniGeneiRn.210040.

Genome annotation databases

EnsembliENSRNOT00000005408; ENSRNOP00000005408; ENSRNOG00000004001.
GeneIDi314548.
KEGGirno:314548.
UCSCiRGD:1305575. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097950 mRNA. Translation: AAH97950.1.
RefSeqiNP_001019958.1. NM_001024787.1.
XP_017451887.1. XM_017596398.1.
UniGeneiRn.210040.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005408.

Proteomic databases

PaxDbiQ4V7F3.
PRIDEiQ4V7F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005408; ENSRNOP00000005408; ENSRNOG00000004001.
GeneIDi314548.
KEGGirno:314548.
UCSCiRGD:1305575. rat.

Organism-specific databases

CTDi64172.
RGDi1305575. Osgepl1.

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
GeneTreeiENSGT00550000075001.
HOGENOMiHOG000109570.
HOVERGENiHBG108218.
InParanoidiQ4V7F3.
KOiK01409.
OMAiICIDHIL.
OrthoDBiEOG091G0HJR.
PhylomeDBiQ4V7F3.

Miscellaneous databases

PROiQ4V7F3.

Gene expression databases

BgeeiENSRNOG00000004001.
GenevisibleiQ4V7F3. RN.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOSGP2_RAT
AccessioniPrimary (citable) accession number: Q4V7F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 5, 2005
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.