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Protein

Centrosomal protein of 55 kDa

Gene

Cep55

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 55 kDa
Short name:
Cep55
Gene namesi
Name:Cep55
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1305340. Cep55.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Cleavage furrow By similarity
  • Midbody By similarity

  • Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. Dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Centrosomal protein of 55 kDaPRO_0000238666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei96 – 961PhosphoserineBy similarity
Modified residuei99 – 991PhosphoserineCombined sources
Modified residuei423 – 4231PhosphoserineBy similarity
Modified residuei426 – 4261PhosphoserineBy similarity
Modified residuei428 – 4281PhosphothreonineBy similarity
Modified residuei434 – 4341Phosphoserine; by PLK1By similarity

Post-translational modificationi

There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4V7C8.
PRIDEiQ4V7C8.

Interactioni

Subunit structurei

Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022133.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 23579Interaction with TSG101By similarityAdd
BLAST
Regioni160 – 21455Interaction with PDCD6IPBy similarityAdd
BLAST
Regioni354 – 462109Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili50 – 185136Sequence analysisAdd
BLAST
Coiled coili228 – 400173Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFBZ. Eukaryota.
ENOG41120Y2. LUCA.
GeneTreeiENSGT00510000047961.
HOGENOMiHOG000111547.
HOVERGENiHBG081092.
InParanoidiQ4V7C8.
KOiK16456.
OMAiSSQRKAD.
OrthoDBiEOG7H4DTJ.
PhylomeDBiQ4V7C8.
TreeFamiTF331107.

Family and domain databases

InterProiIPR022008. EABR.
[Graphical view]
PfamiPF12180. EABR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4V7C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITNGKGK
60 70 80 90 100
VANKDRSKLL EKIQVLEAER EKNVYYLMEK DKEIQRLKDH LRSRYSSSSL
110 120 130 140 150
LEQLEEKTKE CEKKQQLLES LSRETDILKK QLSATTKRLS ELESKASTLH
160 170 180 190 200
LSQSVPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA
210 220 230 240 250
KIFELEKRTE TAAASLPQQM KKTESEGYLQ EEKQKYDHLL ENAKKDLEVE
260 270 280 290 300
RQTVTQLRLE LDEFRRKYEE TQKEVEDLNQ LLSSQRKADI QHLEEDKHKT
310 320 330 340 350
EKIQKLREES SVFKGKLEEE RKKSEELLSQ VRILYDSLLK HQEEQSRVAL
360 370 380 390 400
LERQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH
410 420 430 440 450
GFTFTEQPFP LQGEPENRVK ATSPKSPTAV LNESLVECPK CSVQYPATEH
460
RDLLVHVEYC MK
Length:462
Mass (Da):53,995
Last modified:July 5, 2005 - v1
Checksum:i81F79EA1C4F49FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098013 mRNA. Translation: AAH98013.1.
RefSeqiNP_001020817.1. NM_001025646.1.
XP_006231380.1. XM_006231318.2.
XP_006231381.1. XM_006231319.2.
XP_006231382.1. XM_006231320.2.
UniGeneiRn.90818.

Genome annotation databases

EnsembliENSRNOT00000022133; ENSRNOP00000022133; ENSRNOG00000016377.
GeneIDi294074.
KEGGirno:294074.
UCSCiRGD:1305340. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098013 mRNA. Translation: AAH98013.1.
RefSeqiNP_001020817.1. NM_001025646.1.
XP_006231380.1. XM_006231318.2.
XP_006231381.1. XM_006231319.2.
XP_006231382.1. XM_006231320.2.
UniGeneiRn.90818.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022133.

Proteomic databases

PaxDbiQ4V7C8.
PRIDEiQ4V7C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022133; ENSRNOP00000022133; ENSRNOG00000016377.
GeneIDi294074.
KEGGirno:294074.
UCSCiRGD:1305340. rat.

Organism-specific databases

CTDi55165.
RGDi1305340. Cep55.

Phylogenomic databases

eggNOGiENOG410IFBZ. Eukaryota.
ENOG41120Y2. LUCA.
GeneTreeiENSGT00510000047961.
HOGENOMiHOG000111547.
HOVERGENiHBG081092.
InParanoidiQ4V7C8.
KOiK16456.
OMAiSSQRKAD.
OrthoDBiEOG7H4DTJ.
PhylomeDBiQ4V7C8.
TreeFamiTF331107.

Miscellaneous databases

NextBioi637523.
PROiQ4V7C8.

Family and domain databases

InterProiIPR022008. EABR.
[Graphical view]
PfamiPF12180. EABR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCEP55_RAT
AccessioniPrimary (citable) accession number: Q4V7C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 5, 2005
Last modified: May 11, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.