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Protein

Actin-related protein 3

Gene

Actr3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: RGD
  • ATPase binding Source: RGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • Arp2/3 complex-mediated actin nucleation Source: InterPro
  • astrocyte differentiation Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • cilium morphogenesis Source: UniProtKB
  • negative regulation of axon extension Source: RGD
  • positive regulation of actin cytoskeleton reorganization Source: RGD
  • positive regulation of actin filament polymerization Source: RGD
  • positive regulation of dendrite morphogenesis Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of fibroblast migration Source: RGD
  • positive regulation of filopodium assembly Source: RGD
  • positive regulation of lamellipodium assembly Source: RGD
  • positive regulation of neuron differentiation Source: RGD
  • positive regulation of protein targeting to membrane Source: RGD
  • positive regulation of synapse assembly Source: RGD
  • regulation of myosin II filament organization Source: RGD
  • response to antibiotic Source: RGD
  • response to carbohydrate Source: RGD
  • response to estradiol Source: RGD
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-5663213. RHO GTPases Activate WASPs and WAVEs.
R-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 3
Alternative name(s):
Actin-like protein 3
Gene namesi
Name:Actr3
Synonyms:Arp3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi71024. Actr3.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell projection By similarity

  • Note: In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes).By similarity

GO - Cellular componenti

  • Arp2/3 protein complex Source: InterPro
  • cell-cell junction Source: RGD
  • cell leading edge Source: RGD
  • cytoplasm Source: RGD
  • excitatory synapse Source: RGD
  • Golgi membrane Source: RGD
  • hemidesmosome Source: RGD
  • lamellipodium Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • podosome Source: RGD
  • tubulobulbar complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003423562 – 418Actin-related protein 3Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei240N6-acetyllysineBy similarity1
Modified residuei244N6-acetyllysineBy similarity1
Modified residuei251N6-acetyllysineBy similarity1
Modified residuei254N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ4V7C7.
PRIDEiQ4V7C7.

2D gel databases

World-2DPAGE0004:Q4V7C7.

PTM databases

iPTMnetiQ4V7C7.
PhosphoSitePlusiQ4V7C7.

Expressioni

Tissue specificityi

Detected in brain and kidney glomeruli (at protein level). Detected in kidney, lung and spleen.1 Publication

Gene expression databases

BgeeiENSRNOG00000003206.
ExpressionAtlasiQ4V7C7. baseline and differential.
GenevisibleiQ4V7C7. RN.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Interacts with WHDC1. Interacts weakly with MEFV (By similarity).By similarity

GO - Molecular functioni

  • actin binding Source: RGD
  • ATPase binding Source: RGD

Protein-protein interaction databases

BioGridi249604. 1 interactor.
IntActiQ4V7C7. 2 interactors.
MINTiMINT-3376858.
STRINGi10116.ENSRNOP00000004520.

Structurei

3D structure databases

ProteinModelPortaliQ4V7C7.
SMRiQ4V7C7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0678. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00550000074695.
HOGENOMiHOG000233339.
HOVERGENiHBG003771.
InParanoidiQ4V7C7.
KOiK18584.
PhylomeDBiQ4V7C7.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR015623. Arp3_met.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF253. PTHR11937:SF253. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V7C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVGDQAQRR
60 70 80 90 100
VMKGVDDLDF FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY
110 120 130 140 150
LRAEPEDHYF LLTEPPLNTP ENREYTAEIM FESFNVPGLY IAVQAVLALA
160 170 180 190 200
ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV AEGYVIGSCI KHIPIAGRDI
210 220 230 240 250
TYFIQQLLRD REVGIPPEQS LETAKAVKER YSYVCPDLVK EFNKYDTDGS
260 270 280 290 300
KWIKQYTGVN AISKKEFSID VGYERFLGPE IFFHPEFANP DFTQPISEVV
310 320 330 340 350
DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS
360 370 380 390 400
EELSGGRLKP KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY
410
EEIGPSICRH NPVFGVMS
Length:418
Mass (Da):47,357
Last modified:July 5, 2005 - v1
Checksum:i806FED7A08ABA455
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti111L → F.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292042 mRNA. Translation: BAF94208.1.
AB292043 mRNA. Translation: BAF94209.1.
AB294577 Genomic DNA. Translation: BAF94221.1.
AB294578 Genomic DNA. Translation: BAF94241.1.
BC098014 mRNA. Translation: AAH98014.1.
RefSeqiNP_112330.1. NM_031068.1.
UniGeneiRn.103326.

Genome annotation databases

EnsembliENSRNOT00000004520; ENSRNOP00000004520; ENSRNOG00000003206.
GeneIDi81732.
KEGGirno:81732.
UCSCiRGD:71024. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB292042 mRNA. Translation: BAF94208.1.
AB292043 mRNA. Translation: BAF94209.1.
AB294577 Genomic DNA. Translation: BAF94221.1.
AB294578 Genomic DNA. Translation: BAF94241.1.
BC098014 mRNA. Translation: AAH98014.1.
RefSeqiNP_112330.1. NM_031068.1.
UniGeneiRn.103326.

3D structure databases

ProteinModelPortaliQ4V7C7.
SMRiQ4V7C7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249604. 1 interactor.
IntActiQ4V7C7. 2 interactors.
MINTiMINT-3376858.
STRINGi10116.ENSRNOP00000004520.

PTM databases

iPTMnetiQ4V7C7.
PhosphoSitePlusiQ4V7C7.

2D gel databases

World-2DPAGE0004:Q4V7C7.

Proteomic databases

PaxDbiQ4V7C7.
PRIDEiQ4V7C7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004520; ENSRNOP00000004520; ENSRNOG00000003206.
GeneIDi81732.
KEGGirno:81732.
UCSCiRGD:71024. rat.

Organism-specific databases

CTDi10096.
RGDi71024. Actr3.

Phylogenomic databases

eggNOGiKOG0678. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00550000074695.
HOGENOMiHOG000233339.
HOVERGENiHBG003771.
InParanoidiQ4V7C7.
KOiK18584.
PhylomeDBiQ4V7C7.

Enzyme and pathway databases

ReactomeiR-RNO-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-RNO-3928662. EPHB-mediated forward signaling.
R-RNO-5663213. RHO GTPases Activate WASPs and WAVEs.
R-RNO-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ4V7C7.

Gene expression databases

BgeeiENSRNOG00000003206.
ExpressionAtlasiQ4V7C7. baseline and differential.
GenevisibleiQ4V7C7. RN.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR015623. Arp3_met.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF253. PTHR11937:SF253. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARP3_RAT
AccessioniPrimary (citable) accession number: Q4V7C7
Secondary accession number(s): A9CM89, A9CM90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.